Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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pLIVE® In Vivo Expression and Reporter Vectors

A Vector System Designed to Achieve Long Term Expression in the Livers of Laboratory Mice

Strong Liver Expression of ß-galactosidase from the pLIVE-lacZ Vector after Hydrodynamic Tail Vein Injection

Strong Liver Expression of β-galactosidase from the pLIVE®-lacZ Vector after Hydrodynamic Tail Vein Injection.
The pLIVE®-lacZ Vector (Panel A) was delivered to an ICR mouse using the hydrodynamic tail vein injection procedure and the TransIT®-QR Hydrodynamic Delivery Solution (MIR5240). At 24 hours post-injection, the liver was harvested, sectioned, and stained with X-gal to demonstrate β-galactosidase activity (blue cells). The cells were then counterstained with hematoxylin and eosin Y to stain the nuclei and cytoplasms, respectively. The control mouse (lacZ negative) in Panel B was stained in parallel to Panel A and contains no detectable β-galactosidase activity.

The Promoter in the pLIVE Vector Outperforms the CMV Promoter for Long-term Liver Expression

The Promoter in the pLIVE® Vector Outperforms the CMV Promoter for Long-term Liver Expression.
The pLIVE®-SEAP Vector (20 µg) and pCMV-SEAP (10 µg, CMV promoter-driven SEAP vector) were each delivered to four ICR mice using the hydrodynamic tail vein injection procedure and the TransIT®-QR Hydrodynamic Delivery Solution (MIR5240). At the indicated days post-injection, serum from each mouse was collected and the level of SEAP activity present was determined using the Phospha-Light™ SEAP Assay Kit (Applied Biosystems). The average SEAP activity from each group is presented.

Long-term Expression of Human Placental Secreted Alkaline Phosphatase (SEAP) in Mice

Long-term Expression of Human Placental Secreted Alkaline Phosphatase (SEAP) in Mice after Hydrodynamic Delivery of the pLIVE®-SEAP Vector.
The pLIVE®-SEAP Vector was delivered to three C57Bl/6 mice using the hydrodynamic tail vein injection procedure and the TransIT®-QR Hydrodynamic Delivery Solution (MIR5240). At the indicated times post-injection, serum from each mouse was collected and the level of SEAP activity was determined using the Phospha-Light™ SEAP Assay Kit (Applied Biosystems). Average SEAP activity at each time point is presented.

Features

  • Sustained Gene Expression – Achieve long-term liver gene expression in the mouse liver through 8 months post-injection.
  • Versatile Platform – Available with positive control vectors expressing either LacZ or SEAP.

Description

  • The pLIVE® vector (Liver In Vivo Expression) is designed for high level, prolonged expression of transgenes in the mouse liver. This vector utilizes a chimeric promoter composed of the mouse alpha fetoprotein enhancer II and the minimal mouse albumin promoter. Two introns have been engineered into the vector to increase the expression of the delivered transgene. Downstream of the first intron is a multiple cloning site (MCS) with eight unique restriction sites allowing for simple insertion of the gene of interest. Together the chimeric promoter and two introns are capable of promoting high level transgene expression in the liver for extended lengths of time compared to classic promoters such as the CMV immediate early promoter.
  • In addition to the pLIVE® Vector, two reporter vectors derived from pLIVE, pLIVE-lacZ (encoding β-galactosidase) and pLIVE-SEAP (encoding Secreted Embryonic Alkaline Phosphatase), were created for use as positive controls. Expression of the LacZ protein from pLIVE-lacZ can be monitored in the liver using either classic X-gal staining of liver sections or quantitative β-galactosidase assays of liver lysates. Expression of the SEAP protein from pLIVE-SEAP can be easily monitored using a quantitative assay of mouse serum. The high level, long term liver-specific expression of transgenes from the pLIVE® Expression Vector, as well as the availability of the positive control pLIVE®-lacZ and pLIVE®-SEAP Reporter Vectors, make the pLIVE® Vector series the ideal choice for in vivo liver expression studies in mice.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR5420 pLIVE® Vector 20 µg 419,00 PDF
MIR5520 pLIVE® Vector/lacZ Control Vector Kit 20 µg of each vector 546,00 PDF
MIR5620 pLIVE® Vector/SEAP Control Vector Kit 20 µg of each vector 546,00 PDF
MIR5320 pLIVE® Vector Complete System 20 µg of each (all 3 vectors) 674,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Related Products

Order# Description Amount Price Data Sheet
MIR5210 TransIT®-QR Starter Kit 10 injections 120,00 PDF
MIR5240 TransIT®-QR Delivery Solution 40 injections 177,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

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Vector maps and Sequences

  • Vector map of pLIVE®(pic)
  • Vector map of pLIVE®-lacZ (pic)
  • Vector map of pLIVE®-SEAP (pic)
  • Sequence of pLIVE® (txt)
  • Sequence of pLIVE®-lacZ (txt)
  • Sequence of pLIVE®-SEAP (txt)