Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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PCR Cloning Vector p3T

Vector for PCR Product Cloning

Vector map of p3T
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Vector map of p3T

Features

  • Cloning PCR products via multiple dA extensions
  • Results in higher cloning efficiencies than with single dA/dT overhangs

Description

  • The p3T vector provides a flexible system for the direct cloning of PCR products. It allows cloning of PCR products via multiple dT overhangs. Utilizing the restriction site PflMI, three dT overhangs are produced. After polyadenylating the PCR fragment with Terminal Deoxynucleotidyl Transferase, the PCR fragment can be cloned with high efficiency via a multiple dA extension.
  • Using p3T, less amplified DNA is required. A SmaI site is present to reduce vector background; MscI sites flank the insert for optimal excision. Blue/white selection by alpha -complementation is possible.

  • *PCR is a process covered by patents owned by Hoffmann-La Roche.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
P123T p3T, PCR Product Cloning Vector, lyophilized DNA 5 µg 241,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

  • Shipped at RT; store at 4 °C

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