Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Standard Cloning Vectors

Frequently Used Standard Vectors of High Quality

Scientist looking at colonies on agar plate

Features

  • Select choice of classic standard E. coli vectors
  • Ready-to-use for:
    1. Transformations
    2. Enzymatic reactions
  • Competitive price
  • Of special interest: pUC118 and pUC119 contain additional M13 phage origin for single strand production
 Vector map of pBR322

Vector map of pBR322

  • pBR322 is an ampicillin and tetracycline resistant, general purpose cloning vector. Several inactivating cloning sites are present in the antibiotics resistance genes, which, if a reading frame shift occurs, will give rise to AmpR/TcS or, alternatively, AmpS/TcR transformants. Cloning sites are indicated on the data sheet.
Vector map of pBR325
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Vector map of pBR325

  • pBR325 is an ampicillin, tetracycline and chloramphenicol resistant, general purpose cloning vector. It is derived from pBR322 by insertion (into its EcoR I site) of the chloramphenicol acetyltransferase gene.
Vector map of pBR328
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Vector map of pBR328

  • pBR328 is an ampicillin, tetracycline and chloramphenicol resistant, general purpose cloning vector. This vector, derived from pBR325, has the bom site (basis of mobility) deleted and therefore is non-mobilizable; this makes pBR328 suitable where more stringent biological containment is required. This deletion also creates extra unique cloning sites in the chloramphenicol acetyltransferase gene: Pvu II, BspM II and Bal I.
Vector map of pUC18
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Vector map of pUC18

Vector map of pUC19
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Vector map of pUC19

Vector map of pUC118
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Vector map of pUC118

Vector map of pUC119
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Vector map of pUC119

Vector pUC18/19 and pUC118/119

The plasmids have a multiple cloning site within the lacZ alpha-fragment. Inserts cloned into this site disrupt beta -galactosidase activity and give rise to white colonies on X-Gal/IPTG plates. The plasmids encode resistance to ampicillin. Foreign DNA inserted in-frame with the lac Z gene will be expressed as a fusion protein (containing a portion of the beta-galactosidase) under control of the lac promoter. The promoter is inducible with IPTG and followed by an initiation codon as well as a ribosome binding site. pUC18 and pUC19 differ in their multiple cloning site orientation. pUC118 and pUC119 contain an additional M13 phage origin for single strand production.

Vector map of pACYC184
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Vector map of pACYC184

  • pACYC184 encodes tetracycline and chloramphenicol resistance. Unlike most cloning vectors, which have ColE1 origins of replication, pACYC184 has an origin derived from p15A1. This allows pACYC184 to be maintained in a pBR322 or pUC18 transformant, for example. Such a double transformant is necessary if two recombinant proteins need to be expressed simultaneously.
Vector map of pAT153
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Vector map of pAT153

  • pAT153 is a derivative of pBR322 where the bom (basis of mobility) site has been deleted. Thus pAT153 is non-mobilizable and is more readily contained than pBR322. Also in this 703 bp deletion was the region involved in copy number control; loss of this region gives pAT153 a 1.5 to 3-fold higher copy number. pAT153 encodes ampicillin and tetracycline resistance.

Overview on Vector Features:

Vector Resistance Origin Speciality
pBR322 Tc, Amp ColE1 reading frame shift gives; AmpR , TcS transformants
pBR325 Tc, Amp, Cm ColE1 Cm resistant pBR322
pBR328 Tc, Amp, Cm ColE1 non-mobilizable pBR325
pACYC184 Tc,Cm p15A1 double-transformants with ColE1 vectors
pAT153 Tc, Amp ColE1 non-mobilizable; higher copy than pBR322
pUC18 Amp pMB1 MCS within lacZ: blue/white selection
pUC118 Amp pMB1 M13 origin for ssDNA production
pUC19 Amp pMB1 MCS within lacZ:blue/white selection
pUC119 Amp pMB1 M13 origin for ssDNA production

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount* Price Data Sheet
V30302 pBR322 Vector DNA 25 µg 78,00 PDF
V30402 pBR325 Vector DNA 25 µg 78,00 PDF
V32802 pBR328 Vector DNA 25 µg 78,00 PDF
V32402 pACYC184 Vector DNA 25 µg 78,00 PDF
V32602 pAT153 Vector DNA 25 µg 78,00 PDF
V33002 pUC18 Vector DNA 25 µg 78,00 PDF
V33302 pUC118 Vector DNA 25 µg 78,00 PDF
V33202 pUC19 Vector DNA 25 µg 78,00 PDF
V33402 pUC119 Vector DNA 25 µg 78,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

  • Shipped on blue ice; store at -20 °C; *DNA is in solution