Standard Cloning Vectors
Frequently Used Standard Vectors of High Quality
- Select choice of classic standard E. coli vectors
- Ready-to-use for:
- Enzymatic reactions
Vector map of pBR322
- pBR322 is an ampicillin and tetracycline resistant, general purpose cloning vector. Several inactivating cloning sites are present in the antibiotics resistance genes, which, if a reading frame shift occurs, will give rise to AmpR/TcS or, alternatively, AmpS/TcR transformants. Cloning sites are indicated on the data sheet.
Vector map of pBR325
- pBR325 is an ampicillin, tetracycline and chloramphenicol resistant, general purpose cloning vector. It is derived from pBR322 by insertion (into its EcoR I site) of the chloramphenicol acetyltransferase gene.
Vector map of pBR328
- pBR328 is an ampicillin, tetracycline and chloramphenicol resistant, general purpose cloning vector. This vector, derived from pBR325, has the bom site (basis of mobility) deleted and therefore is non-mobilizable; this makes pBR328 suitable where more stringent biological containment is required. This deletion also creates extra unique cloning sites in the chloramphenicol acetyltransferase gene: Pvu II, BspM II and Bal I.
Vector pUC18/19 and pUC118/119
The plasmids have a multiple cloning site within the lacZ alpha-fragment. Inserts cloned into this site disrupt beta -galactosidase activity and give rise to white colonies on X-Gal/IPTG plates. The plasmids encode resistance to ampicillin. Foreign DNA inserted in-frame with the lac Z gene will be expressed as a fusion protein (containing a portion of the beta-galactosidase) under control of the lac promoter. The promoter is inducible with IPTG and followed by an initiation codon as well as a ribosome binding site. pUC18 and pUC19 differ in their multiple cloning site orientation. pUC118 and pUC119 contain an additional M13 phage origin for single strand production.
Vector map of pACYC184
- pACYC184 encodes tetracycline and chloramphenicol resistance. Unlike most cloning vectors, which have ColE1 origins of replication, pACYC184 has an origin derived from p15A1. This allows pACYC184 to be maintained in a pBR322 or pUC18 transformant, for example. Such a double transformant is necessary if two recombinant proteins need to be expressed simultaneously.
Vector map of pAT153
- pAT153 is a derivative of pBR322 where the bom (basis of mobility) site has been deleted. Thus pAT153 is non-mobilizable and is more readily contained than pBR322. Also in this 703 bp deletion was the region involved in copy number control; loss of this region gives pAT153 a 1.5 to 3-fold higher copy number. pAT153 encodes ampicillin and tetracycline resistance.
Overview on Vector Features:
|pBR322||Tc, Amp||ColE1||reading frame shift gives; AmpR , TcS transformants|
|pBR325||Tc, Amp, Cm||ColE1||Cm resistant pBR322|
|pBR328||Tc, Amp, Cm||ColE1||non-mobilizable pBR325|
|pACYC184||Tc,Cm||p15A1||double-transformants with ColE1 vectors|
|pAT153||Tc, Amp||ColE1||non-mobilizable; higher copy than pBR322|
|pUC18||Amp||pMB1||MCS within lacZ: blue/white selection|
|pUC118||Amp||pMB1||M13 origin for ssDNA production|
|pUC19||Amp||pMB1||MCS within lacZ:blue/white selection|
|pUC119||Amp||pMB1||M13 origin for ssDNA production|
- For shipping and storage information please click on Order#.
|V30302||pBR322 Vector DNA||25 µg||78,00|
|V30402||pBR325 Vector DNA||25 µg||78,00|
|V32802||pBR328 Vector DNA||25 µg||78,00|
|V32402||pACYC184 Vector DNA||25 µg||78,00|
|V32602||pAT153 Vector DNA||25 µg||78,00|
|V33002||pUC18 Vector DNA||25 µg||78,00|
|V33302||pUC118 Vector DNA||25 µg||78,00|
|V33202||pUC19 Vector DNA||25 µg||78,00|
|V33402||pUC119 Vector DNA||25 µg||78,00|
- All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.
- Shipped on blue ice; store at -20 °C; *DNA is in solution