Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Vectors for Expression of Human Hec1 Complex Fusion Proteins

Expression of EGFP Fusion Proteins for the Investigation of the Hec1 Complex

Vector map of pG-22-hHec1

Vector map of pG-22-hHec1

Schematic view of the Hec1 complex

Schematic view of the Hec1 complex

Features

  • Ready-to-use vectors for live cell imaging
  • Large selection of genes
  • Sequence-documented vectors
  • Applications include localization, binding, and protein-protein interaction studies
  • Experimentally approved

Description

  • At each centromere, a multi-protein complex binds named the kinetochore. The inner kinetochore directly binds to the centromeric chromatin during the whole cell cycle. During mitosis, further proteins of the outer kinetochore, including components of the Hec1 complex, bind to the inner kinetochore and establish the stable link between the centromeric chromatin and the microtubuli. This mechanical connection is regulated by proteins of the mitotic checkpoint. MoBiTec offers expression vectors coding for human Hec1 complex proteins in fusion with EGFP. Most of the fusion proteins can be expressed either with N- or C-terminal EGFP-tag. The cipher in the vector name indicates the linker length of the fusion proteins (e.g., for vector pG-22-hHec1 the linker length is 22 amino acids).

Application

  • The Hec1 complex EGFP fusion proteins can be used in modern fluorescence microscopy techniques to determine
    1. localization,
    2. binding,
    3. dynamics, and, when a second color is available,
    4. protein-protein interactions and
    5. protein-protein proximities
  • applying fluorescence methods like FRET, FRAP, FCS, RICS, FCCS, and F3H.

Please note:

  • For FRET analysis we suggest fusion proteins with short linker.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.

Hec1

Order# Description Amount Price Data Sheet
VS-HEV00001 pG-22-hHec1 15 ug 453,00 PDF

Nuf2

Order# Description Amount Price Data Sheet
VS-HEV00021 pG-20-hNuf2 15 ug 453,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

Literature

  • Foley EA, Kapoor TM (2013): Microtubule attachment and spindle assembly checkpoint signaling at the kinetochore. Nat Rev Mol Cell Biol. 2013 Jan;14(1):25-37. Pubmed
  • Takeuchi K, Fukagawa T (2012): Molecular architecture of vertebrate kinetochores. Exp Cell Res. 2012 Jul 15;318(12):1367-74. Pubmed
  • Varma D. and Salmon E.D (2012): The KMN protein network-chief conductors of the kinetochore orchestra. Journal of Cell Science 2012 Dec 15; 125, 5927–5936. Pubmed