Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Vectors for Expression of Human Mis12 Complex Fusion Proteins

Expression of EGFP Fusion Proteins for the Investigation of the Human Mis12 Complex

Vector map of pG-20-hMis12

Vector map of pG-20-hMis12

Schematic view of the Mis12 complex

Schematic view of the Mis12 complex

Features

  • Ready-to-use vectors for live cell imaging
  • Large selection of genes
  • Sequence-documented vectors
  • Applications include localization, binding, and protein-protein interaction studies
  • Experimentally approved

Description

  • At each centromere, a multi-protein complex binds named the kinetochore. The inner kinetochore directly binds to the centromeric chromatin during the whole cell cycle. During mitosis, further proteins of the outer kinetochore bind to the inner kinetochore and establish the stable link between the centromeric chromatin and the microtubuli. This mechanical connection is regulated by proteins of the mitotic checkpoint. The Mis12 complex is a link between the inner and outer kinetochore. MoBiTec offers human Mis12 complex proteins in fusion with EGFP. Most of the fusion proteins can be expressed either with N- or C-terminal EGFP-tag. The cipher in the vector name indicates the linker length of the fusion proteins (e.g., for vector pG-22-hMis12 the linker length is 22 amino acids).

Application

  • The Mis12 complex EGFP fusion proteins can be used in modern fluorescence microscopy techniques to determine
    1. localization,
    2. binding,
    3. dynamics, and, when a second color is available,
    4. protein-protein interactions and
    5. protein-protein proximities
  • applying fluorescence methods like FRET, FRAP, FCS, RICS, FCCS, and F3H.

Please note:

  • For FRET analysis we suggest fusion proteins with short linker.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.

Dsn1

Order# Description Amount Price Data Sheet
VS-MSV00001 pG-22-hDsn1 15 ug 453,00 PDF

Mis12

Order# Description Amount Price Data Sheet
VS-MSV00021 pG-22-hMis12 15 ug 453,00 PDF
VS-MSV00022 phMis12-15-G 15 ug 453,00 PDF

Nnf1

Order# Description Amount Price Data Sheet
VS-MSV00041 pG-20-hNnf1 15 ug 453,00 PDF
VS-MSV00042 phNnf1-15-G 15 ug 453,00 PDF

Nsl1

Order# Description Amount Price Data Sheet
VS-MSV00061 pG-20-hNsl1 15 ug 453,00 PDF
VS-MSV00062 phNsl1-15-G 15 ug 453,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

Literature

  • Foley EA, Kapoor TM (2013): Microtubule attachment and spindle assembly checkpoint signaling at the kinetochore. Nat Rev Mol Cell Biol. 2013 Jan;14(1):25-37. Pubmed
  • Takeuchi K, Fukagawa T (2012): Molecular architecture of vertebrate kinetochores. Exp Cell Res. 2012 Jul 15;318(12):1367-74. Pubmed
  • Varma D. and Salmon E.D (2012): The KMN protein network-chief conductors of the kinetochore orchestra. Journal of Cell Science 2012 Dec 15; 125, 5927–5936. Pubmed