Vector map of pG-20-hMis12
Schematic view of the Mis12 complex
- Ready-to-use vectors for live cell imaging
- Large selection of genes
- Sequence-documented vectors
- Applications include localization, binding, and protein-protein interaction studies
- Experimentally approved
- At each centromere, a multi-protein complex binds named the kinetochore. The inner kinetochore directly binds to the centromeric chromatin during the whole cell cycle. During mitosis, further proteins of
the outer kinetochore bind to the inner kinetochore and establish the stable link between the centromeric chromatin and the microtubuli. This mechanical connection is regulated by proteins of the mitotic
checkpoint. The Mis12 complex is a link between the inner and outer kinetochore. MoBiTec offers human Mis12 complex proteins in fusion with EGFP. Most of the fusion proteins can be expressed either with N-
or C-terminal EGFP-tag. The cipher in the vector name indicates the linker length of the fusion proteins (e.g., for vector pG-22-hMis12 the linker length is 22 amino acids).
- The Mis12 complex EGFP fusion proteins can be used in modern fluorescence microscopy techniques to determine
applying fluorescence methods like FRET, FRAP, FCS, RICS, FCCS, and F3H.
- dynamics, and, when a second color is available,
- protein-protein interactions and
- protein-protein proximities
- For FRET analysis we suggest fusion proteins with short linker.