Vector map of pG-20-hCdc20
Three dimensional kinetochore model by Döring (2012)
- Ready-to-use vectors for live cell imaging
- Large selection of genes
- Sequence-documented vectors
- Applications include localization, binding, and protein-protein interaction studies
- Experimentally approved
- At each centromere, a multi-protein complex binds named the kinetochore. The inner kinetochore directly binds to the centromeric chromatin during the whole cell cycle. During mitosis, further proteins of
the outer kinetochore bind to the inner kinetochore and establish the stable link between the centromeric chromatin and the microtubuli. This mechanical connection is regulated by proteins of the mitotic
checkpoint. MoBiTec offers a large number of human mitotic checkpoint proteins in fusion with EGFP. Most of the fusion proteins can be expressed either with N- or C-terminal EGFP-tag. The cipher in the vector
name indicates the linker length of the fusion proteins (e.g., for vector pG-20-hCdc20 the linker length is 20 amino acids).
- The mitotic checkpoint EGFP fusion proteins can be used in modern fluorescence microscopy techniques to determine
applying fluorescence methods like FRET, FRAP, FCS, RICS, FCCS, and F3H.
- dynamics, and, when a second color is available,
- protein-protein interactions and
- protein-protein proximities
- For FRET analysis we suggest fusion proteins with short linker.