Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

EndoClear™ & EndoClear™ Plus Recombinant Human Alpha-Synuclein Protein

Are Endotoxins Interfering with Your Critical Neuroscience Research?

EndoClear-Intro
LewyBody

Lewy Body in a Neuronal Cell
Lewy bodies are abnormal aggregates of α-synuclein. They are observed inside nerve cells in Parkinson's disease, Lewy body dementia, and other disorders

  • EndoClear™ and EndoClear™ Plus human alpha-synuclein recombinant protein contain exceptional low levels of endotoxin equal to 0.1 EU/µg protein and 0.001 EU/µg protein respectively.

Features

  • The lowest endotoxin levels in research-grade alpha-synuclein
  • Purity greater than 95%
  • Highly stable
  • Minimizes potentially negative effects on cell-culture and in vivo experiments
  • Thioflavin-T binding fibrillation proven research-grade product (every lot checked for fibrillation capability)

Application

  • • Fibrillation
  • • Oligomerization
  • • Cell cytotoxicity

About Endotoxins

  • Did you know that Endotoxin can affect cell cultures? Most commercial recombinant proteins produced in bacteria contain inherent/residual endotoxin concentrations that create a variety of problems for researchers using cell culture, not limited to the following:
  • Human T-cells Induction of proliferation, lymphokine production
  • Cardiac myocytes Induced contractile dysfunction
  • Macrophages Induced IL-6 production
  • B Lymphomas Increased production of immunoglobulin light chains
  • Recombinant CHO Altered protein production

  • Potential sources of endotoxin contamination in cell cultures include media and its components, water, sera, glassware, and plasticware. Endotoxins are amphipathic molecules with a net negative charge in solution. Their properties include:
  • • Heat stability
  • • Large aggregate-forming ability
  • • Strong affinities to hydrophobic materials like plastic

About Alpha-Synuclein

  • Alpha-Synuclein is a central molecule responsible for neurodegenerative effects observed in α-synucleopathies. It presents a major component of Lewy bodies in affected neurons in Parkinson's disease.
  • It is believed that α-synuclein exists - under physiological conditions - in random coils. Under pathological conditions, the native protein undergoes misfolding (beyond proteasomal repair) to form dimers/trimers/oligomers which in turn aggregate into higher-ordered protofibril structures. These serve as building blocks for pathological inclusions of α-synuclein in the neurons.
  • Alpha-Synuclein is a 14.5 kDa (140 amino acids long) protein predominantly expressed in brain, specifically in cerebellum, thalamus, neocortex, hippocampus, and striatum regions.
  • Alpha-Synuclein structure consists of 3 domains:
  • • N-terminal (amphipathic) with repeats including KTKEGV consensus sequence important for α-helix formation
  • • Central hydrophobic region with the non-amyloid component region important for protein aggregation
  • • C-terminal region which is highly acidic and proline-rich

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
55555-100AS EndoFree Recombinant Human alpha-Synuclein Protein 100 µg 141,00 PDF
55555-500AS EndoFree Recombinant Human alpha-Synuclein Protein 500 µg 426,00 PDF
55555-1000AS EndoFree Recombinant Human alpha-Synuclein Protein 1000 µg 708,00 PDF
56081-100AS EndoClearTMPlus Recombinant Human alpha-Synuclein Protein 100 µg 256,00 PDF
56081-500AS EndoClearTMPlus Recombinant Human alpha-Synuclein Protein 500 µg 642,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Related Products

Order# Description Amount Price Data Sheet
56083-100AS EndoClear™ Rat Recombinant alpha-Synuclein Protein 100 µg 141,00 PDF
56083-500AS EndoClear™ Rat Recombinant alpha-Synuclein Protein 500 µg 426,00 PDF
56083-1000AS EndoClear™ Rat Recombinant alpha-Synuclein Protein 1000 µg 708,00 PDF
56082-100AS EndoClear™ Mouse Recombinant alpha-Synuclein Protein 100 µg 141,00 PDF
56082-500AS EndoClear™ Mouse Recombinant alpha-Synuclein Protein 500 µg 426,00 PDF
56082-1000AS EndoClear™ Mouse Recombinant alpha-Synuclein Protein 1000 µg 708,00 PDF
55457AS Recombinant human α-Synuclein (1-140), HiLyte™ Fluor 488 labeled 200 µg 653,00 PDF
55458-100AS Recombinant Human β-Synuclein (1-134) 0.1 mg 161,00 PDF
55458-500AS Recombinant Human β-Synuclein (1-134) 0.5 mg 338,00 PDF
55458-1000AS Recombinant Human β-Synuclein (1-134) 1 mg 540,00 PDF
55581AS Recombinant human α-Synuclein (1-140), biotin labeled   200 µg 594,00 PDF
55550-HAS SensoLyte® Anti- α-Synuclein (Human) ELISA Kit 1 kit 557,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Download

AnaSpec Brochures Icon
  • AnaSpec: Protein iDentification Brochure (PDF)
  • AnaSpec: Peptides Brochure (PDF)
  • AnaSpec: TR-FRET Flyer (PDF)