GeneTrans Transfection Reagents
GeneTrans Gene Delivery Reagent
- High transfection efficiencies in multiple cell lines
- Good results with many hard-to-transfect cell lines
- Very cost-effective and easy-to-use
- Best efficiency with Jurkat cells
- Ideal for oligonucleotide transfection
- GeneTrans Transfection Reagents have been developed for highly efficient transfection of a broad variety of cell types. They enable good transfection efficiencies also with many known hard-to-transfect cell lines
- GeneTrans Transfection Reagents utilize the unique Direct Hydrophilic Conjugation (DHC) technology developed by the inventor of lipofection. In contrast to other commercially available transfection reagents, GeneTrans contains an ideally adjusted mixture of differentially modified cationic lipids. It optimizes the assembly of lipid/DNA complexes and significantly reduces cytotoxicity. This ensures highly efficient and reproducible results with both stable and transient transfections. Moreover, the strongly reduced cell damage and the elimination of media changes make GeneTrans Transfection Reagents ideal for all gene expression studies where both optimal gene delivery and the post-transfection state of the cells are important. In addition, they have proven to be perfect for oligonucleotide transfection and for transfecting special cell lines such as Jurkat cells.
delivery into cells.
GeneTrans II Transfection Reagent
- Optimized transfection efficiency
- Greatly improved results with hard-to-transfect cells
- Ideally suited for oligonucleotide transfection
- No media changes - transfections can be easily carried out with or without serum
- Robust reproducible results with cell lines used for protein expression
- Adaptable to both suspension and adherent cells
- Minimized cytotoxicity
- Suitable for both in vitro and in vivo transfection
- Excellent and reproducible performance with many different cell lines
- The GeneTrans II Transfection Reagent is an advance in gene delivery technology and based on a clearly improved formulation that allows a further enhancement of DNA stability and reduction of interference from serum components during the transfection process. Its ability first to stabilize and protect DNA more effectively and second, to facilitate its nuclear uptake into cells renders GeneTrans II a highly effective delivery tool. Additionally, lipoplexes produced with the GeneTrans II formulation have lower interaction with serum components and are thereby in particular very suitable and effective for use in serum containing medium. It includes optimized components and buffers allowing superior transfection efficiencies also with notoriously hard-to-transfect cell lines such as HeLa, K-562, and PC-12.
- GeneTrans II is ideal for high-throughput screening and protein expression applications and offers clear advantages compared to other leading transfection reagents. GeneTrans II is provided with two diluents (DNA Diluent A and DNA Diluent B) to allow you more flexibility in optimizing nucleic acid transfections for your cell lines of choice.
- GeneTrans II comes complete with everything you need for optimizing transfection conditions for your specific cell line(s), including the convenient GeneTrans II dried lipid films, hydration buffer (0.75 ml or 1.5 ml), and two types of DNA diluents (A and B: 4 ml or 8 ml). For your convenience, a protocol with tested optimized transfection conditions for the most popularly used cell lines is included. By following the simple steps in this comprehensive manual, you can expect optimal transfection results.
Comparison of GeneTrans II Transfection reagent with leading reagent L2K.
- For shipping and storage information please click on Order#.
|0201B||GeneTrans II Transfection Reagent||75 reactions (0.75ml)||278,00|
|0202B||GeneTrans II Transfection Reagent||150 reactions (1.5ml)||523,00|
|0203B||GeneTrans II Transfection Reagent||750 reactions (5 x 1.5ml)||2191,00|
|0204B||GeneTrans II QuickEase Kit||96 Single Use Vials||411,00|
- All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.
- Agoston Z, Heine P, Brill MS, Grebbin BM, Hau AC, Kallenborn-Gerhardt W, Schramm J, Götz M, and Schulte D. Meis2 is a Pax6 co-factor in neurogenesis and dopaminergic periglomerular fate specification in the adult olfactory bulb. Development. 2014 Jan;141(1):28-38.
- Daher J, Martin M, Rousseau A, Nuyens V, Kazan HF, Van Antwerpen P, Courbebaisse G, Martiat P, Badran B, Dequiedt F, Boudjeltia KZ, and Vanhamme L. Myeloperoxidase Oxidized LDL Interferes with Endothelial Cell Motility through miR-22 and Heme Oxygenase 1 Induction: Possible Involvement in Reendothelialization of Vascular Injuries Hindawi Publishing Corporation, Mediators of Inflammation, Volume 2014, Article ID 134635, 14 pages
- Grassmann F, Schoenberger PGA, Brandl C, Schick T, Hasler D, et al. A Circulating MicroRNA Profile Is Associated with Late-Stage Neovascular Age-Related Macular Degeneration. PLoS ONE, 2014 9(9): e107461.
- Spinnenhirn V, Farhan H, Basler M, Aichem A, Canaan A, Groettrup M. The ubiquitin-like modifier FAT10 decorates autophagy-targeted Salmonella and contributes to Salmonella resistance in mice J. Cell Sci., Nov 2014; 127: 4883 - 4893.
- Guo L, Xu J, Qi J, Zhang L, Wang J, Liang J, Qian N, Zhou H, Wei L, Deng L. MicroRNA-17-92a upregulation by estrogen leads to Bim targeting and inhibition of osteoblast apoptosis J. Cell Sci., Feb 2013; 126: 978 - 988.
- Boeckel JN, Guarani V, Koyanagi M, Roexe T, Lengeling A, Schermuly RT, Gellert P, Braun T, Zeiher A, Dimmeler S. Jumonji domain-containing protein 6 (Jmjd6) is required for angiogenic sprouting and regulates splicing of VEGF-receptor 1 PNAS, Feb 2011; 108: 3276 - 3281.
- Froese N, Kattih B, Breitbart A, Grund A, Geffers R, Molkentin JD, Kispert A, Wollert KC, Drexler H, Heineke J. GATA6 Promotes Angiogenic Function and Survival in Endothelial Cells by Suppression of Autocrine Transforming Growth Factor ß/Activin Receptor-like Kinase 5 Signaling J. Biol. Chem., Feb 2011; 286: 5680 - 5690.
- Kaluza D, Kroll J, Gesierich S, Yao TP, Boon RA, Hergenreider E, Tjwa M, Rössig L, Seto E, Augustin HG, Zeiher AM, Dimmeler S, Urbich C. Class IIb HDAC6 regulates endothelial cell migration and angiogenesis by deacetylation of cortactin EMBO J., Oct 2011; 30: 4142 - 4156.
- Carmona G, Göttig S, Orlandi A, Scheele J, Bäuerle T, Jugold M, Kiessling F, Henschler R, Zeiher AM, Dimmeler S, Chavakis E. Role of the small GTPase Rap1 for integrin activity regulation in endothelial cells and angiogenesis Blood, Jan 2009; 113: 488 - 497
- Phng LK, Potente M, Leslie JD, Babbage J, Nyqvist D, Lobov I, Ondr JK, Rao S, Lang RA, Thurston G, Gerhardt H. Nrarp Coordinates Endothelial Notch and Wnt Signaling to Control Vessel Density in Angiogenesis Dev. Cell, Vol 16, 1, Jan 2009; 70–82.
- Schröder K, Kohnen A, Aicher A, Liehn EA, Büchse T, Stein S, Weber C, Dimmeler S, Brandes RP. NADPH Oxidase Nox2 Is Required for Hypoxia-Induced Mobilization of Endothelial Progenitor Cells Circ. Res. 2009;105;537-544.
- Urbich C, Rössig L, Kaluza D, Potente M, Boeckel JN, Knau A, Diehl F, Geng JG,Hofmann WK, M. Zeiher AM, Dimmeler S. HDAC5 is a repressor of angiogenesis and determines the angiogenic gene expression pattern of endothelial cells Blood, May 2009; 113: 5669 - 5679.
- Martin M, Potente M, Janssens V, Vertommen D, Twizere JC, Rider MH, Goris J, Dimmeler S, Kettmann R, Dequiedt F Protein phosphatase 2A controls the activity of histone deacetylase 7 during T cell apoptosis and angiogenesis PNAS, Mar 2008; 105: 4727 - 4732.
- Michaelis UR, Xia N, Barbosa-Sicard E, Falck JR, Fleming I Role of Cytochrome P450 2C Epoxygenases in Hypoxia-Induced Cell Migration and Angiogenesis in Retinal Endothelial Cells Invest. Ophthalmol. Vis. Sci., Mar 2008; 49: 1242 - 1247.
- Webler AC, Michaelis UR, Popp R, Barbosa-Sicard E, Murugan A, Falck JR, Fisslthaler B, Fleming I. Epoxyeicosatrienoic acids are part of the VEGF-activated signaling cascade leading to angiogenesis. Am J Physiol Cell Physiol. 2008 Nov;295(5):C1292-301
- Webler AC, Popp R, Korff T, Michaelis UR, Urbich C, Busse R, and Fleming I. Cytochrome P450 2C9-Induced Angiogenesis Is Dependent on EphB4 Arterioscler Thromb Vasc Biol, Jun 2008; 28: 1123 - 1129.
- Diehl F, Rössig L, Zeiher AM, Dimmeler S, Urbich C The histone methyltransferase MLL is an upstream regulator of endothelial-cell sprout formation Blood, Feb 2007; 109: 1472 - 1478.
- Kuehbacher A, Urbich C, Zeiher AM, Dimmeler S. Role of Dicer and Drosha for Endothelial MicroRNA Expression and Angiogenesis Circ. Res., Jul 2007; 101: 59 - 68.
- Potente M, Ghaeni L, Baldessari D, Mostoslavsky R, Rossig L, Dequiedt F, Haendeler J, Mione M, Dejana E, Alt FW, Zeiher AM, Dimmeler S. SIRT1 controls endothelial angiogenic functions during vascular growth Genes & Dev., Oct 2007; 21: 2644 - 2658.
- Fleming I, Fisslthaler B, Dixit M, Busse R. Role of PECAM-1 in the shear-stress-induced activation of Akt and the endothelial nitric oxide synthase (eNOS) in endothelial cells J. Cell Sci., Sep 2005; 118: 4103 - 4111.
- Michaelis UR, Fisslthaler B, Barbosa-Sicard E, Falck JR, Fleming I, Busse R Cytochrome P450 epoxygenases 2C8 and 2C9 are implicated in hypoxia-induced endothelial cell migration and angiogenesis J. Cell Sci., Dec 2005; 118: 5489 - 5498.
- Bruhl T, Urbich C, Aicher D, Acker-Palmer A, Zeiher AM, Dimmeler S Homeobox A9 Transcriptionally Regulates the EphB4 Receptor to Modulate Endothelial Cell Migration and Tube Formation Circ. Res., Apr 2004; 94: 743 - 751.
- Brühl T, Heeschen C, Aicher A, Jadidi AS, Haendeler J, Hoffmann J, Schneider MD, Zeiher AM, Dimmeler S, Rössig L. p21Cip1 Levels Differentially Regulate Turnover of Mature Endothelial Cells, Endothelial Progenitor Cells, and In Vivo Neovascularization Circ. Res., Mar 2004; 94: 686 - 692.
- Dernbach E, Urbich C, Brandes RP, Hofmann WK, Zeiher AM, and Dimmeler S. Antioxidative stress–associated genes in circulating progenitor cells: evidence for enhanced resistance against oxidative stress Blood, Dec 2004; 104: 3591 - 3597.