Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology


TransIT®-2020 Transfection Reagent

A High Performance, Animal-Free, Broad Spectrum DNA Transfection Reagent


  • Superior Performance - TransIT®-2020 outperforms FuGENE® HD, Lipofectamine™ 2000, and Lipofectamine™ 2000 CD
  • Broad Spectrum DNA Delivery - Achieve high expression in many cell types, including hard to transfect cells
  • Animal Origin Free - TransIT®-2020 provides high performance with maximum compatibility
  • Scientifically Proven - Developed by Mirus Bio scientists with over 15 years of gene delivery expertise


  • TransIT®-2020 Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells. TransIT®-2020 affords high levels of gene expression in typically hard-to-transfect cell types, including primary cells and is suitable for both transient and stable transfection. TransIT®-2020 is composed of animal-origin free components and is serum compatible, eliminating the need for any culture medium change after transfection.
HEK 293
Primary MEF
  • Superior Gene Expression in a Broad Spectrum of Cell Types Using TransIT®-2020
    Transfection Reagent.

    Luciferase gene expression was compared in HEK 293, K562, CHO-K1, and primary MEF cells transfected with a luciferase expression plasmid using TransIT®-2020 (Mirus Bio, 3:1 reagent to DNA ratio), FuGENE® HD (Roche, 7:2 reagent to DNA ratio), Lipofectamine™ 2000 (Invitrogen, 5:2 reagent to DNA ratio), and Lipofectamine™ 2000 CD (Invitrogen, 5:2 reagent to DNA ratio). Transfections were performed in 24-well plates using 0.5 µg of plasmid DNA per well. The optimal level of each transfection reagent was determined empirically and all reagents were used according to manufacturer’s protocol. Cells were harvested at 24 hours post-transfection and assayed for luciferase activity. Error bars represent the standard deviation of triplicate wells.


  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR5404 TransIT®-2020 Transfection Reagent 0.4 ml 221,00 PDF
MIR5400 TransIT®-2020 Transfection Reagent 1 ml 437,00 PDF
MIR5405 TransIT®-2020 Transfection Reagent 5 x 1 ml 1906,00 PDF
MIR5406 TransIT®-2020 Transfection Reagent 10 x 1 ml 3524,00 PDF
MIR5410 TransIT®-2020 Transfection Reagent 0.1 ml 0,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

  • * Sample Policy:
    MoBiTec together with Mirus considers sample requests for up to three different transfection products and one electroporation product per laboratory. Please include all samples to be tested in a single request.