Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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TransIT®-TKO: Small Interfering RNA (siRNA) Transfection Reagent

For High-Efficient Small Interfering RNA Transfection

Knock out of firefly luciferase expression in 3T3-luc cells using TransIT®-TKO

3T3-luc, a cell line stably expressing the firefly luciferase gene, was used to test the ability of TransIT®-TKO reagent/siRNA complexes to knock out expression of an existing target gene.

Features

  • Delivers siRNA targeted against both transient transfected and stable genes in a variety of cell lines while not effecting untargeted expression of similar genes - significantly reduced cell damage
  • Ideal for all siRNA-mediated gene silencing studies
  • No media change or serum addition required

Description

  • The TransIT®-TKO reagent enables highly efficient siRNA transfection with significantly reduced levels of cell damage as compared to cationic-liposome based transfection reagents. Transfections are most effective when carried out in complete growth media without a media change or serum addition.
  • When complexed with even low levels of siRNA, TransIT®-TKO reagent successfully knocks out target gene expression in a variety of cell lines. Transgene expression can be silenced either when the siRNA is transfected before, concurrently or after the transfection of the expression vector. These unique features make TransIT®-TKO transfection reagent ideal for all siRNA-mediated gene-silencing studies. It is the only transfection reagent designed to efficiently deliver small interfering RNA to cells in vitro.
  • TransIT®-TKO reagent, combined with the correct siRNA sequence against the target mRNA, can silence endogenous, stable or transient gene expression.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR2154 TransIT-TKO® Transfection Reagent 0.4 ml 276,00 PDF
MIR2150 TransIT-TKO® Transfection Reagent 1.5 ml 442,00 PDF
MIR2155 TransIT-TKO® Transfection Reagent 5 x 1.5 ml 1920,00 PDF
MIR2156 TransIT-TKO® Transfection Reagent 10 x 1.5 ml 3549,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.
  • * Sample Policy:
    MoBiTec together with Mirus considers sample requests for up to three different transfection products and one electroporation product per laboratory. Please include all samples to be tested in a single request.