Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

TransIT®-BrCa Transfection Reagent

First dedicated Breast Cancer Cell Line specific Reagent available on the market!

Transfection of plasmid DNA into different cell lines with TransIT®-BrCa

Fig. 1 TransIT®-BrCa Transfection Reagent Yields High Efficiency Plasmid DNA Transfection.
TransIT®-BrCa Transfection Reagent was used to transfect plasmid DNA encoding GFP into MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D breast cancer cell lines. Transfections were performed in 24-well plates using 1-1.5 µl of TransIT®-BrCa Transfection Reagent to deliver 0.5 µg of DNA (2:1 and 3:1, reagent:DNA ratio). Triplicate wells were assayed 48 hours post-transfection on a guava® easyCyte™ 5HT flow cytometer.

MCF7

Fig. 2 MCF7

MDA-MB-231

Fig. 3 MDA-MB-231

MDA-MB-453

Fig. 4 MDA-MB-453

MDA-MB-468

Fig. 5 MDA-MB-468

T47D

Fig. 6 T47D

Features

  • Superior DNA Delivery - Achieve high expression levels in breast cancer cell types including: MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D
  • Formulated for Low Cellular Toxicity - Maintain cell density and reduce experimental biases due to toxicity
  • Scientifically Proven - Developed by Mirus Bio scientists with nearly 2 decades of gene delivery expertise

Description

  • TransIT®-BrCa Transfection Reagent is the first dedicated breast cancer cell line specific reagent available to the market today. While it delivers efficiently to many breast cancer cell types, it also maintains low cellular toxicity resulting in reduced experimental biases.

Fig. 2-6 TransIT®-BrCa Transfection Reagent Exhibits Higher Luciferase Expression in Breast Cancer Cells Compared to Other Transfection Reagents.
Breast Cancer cell lines, MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D, were transfected with a luciferase expression plasmid using the designated reagents at the reagent-to-DNA ratios indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression was measured at 24 hours post-transfection using a standard assay. Error bars represent the standard deviation of triplicate wells.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR5504 TransIT®-BrCa Transfection Reagent 0.4 ml 235,00 PDF
MIR5500 TransIT®-BrCa Transfection Reagent 1 ml 356,00 PDF
MIR5505 TransIT®-BrCa Transfection Reagent 5 x 1 ml 1552,00 PDF
MIR5506 TransIT®-BrCa Transfection Reagent 10 x 1 ml 2870,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.
  • * Sample Policy:
    MoBiTec together with Mirus considers sample requests for up to three different transfection products and one electroporation product per laboratory. Please include all samples to be tested in a single request.

Download

  • Mirus TransIT®-BrCa Flyer (PDF)
  • Mirus Transfection Brochure 2013 (PDF)