Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Ingenio® Electroporation Products

High Efficiency Electroporation Using Any Instrument

Features

  • High Efficiency Electroporation of Hard-to-Transfect Cells – Including primary cells and stem cells
  • Ideal for plasmid or siRNA electroporation
  • Compatibility with Most Conventional Electroporation Devices – Such as Lonza-Amaxa®, Bio-Rad®, or Harvard BTX®
  • Cost Savings Using the Ingenio® Electroporation Kits – On your existing Amaxa® Nucleofector® without sacrificing performance
  • Longer Shelf Life than Other Products – One year shelf life allows maximum usage for your experiments
  • Buy Only What You Need – Available as solution alone or as part of a kit with cuvettes and cell droppers

Description

  • Mirus Bio has developed the Ingenio® Electroporation Solution to facilitate efficient and reliable delivery of nucleic acids to eukaryotic cells traditionally resistant to chemical transfection. Ingenio® is a broad spectrum solution that supports high efficiency electroporation with minimal toxicity. It replaces standard electroporation solutions, including phosphate buffered saline and serum-free media. Ingenio® is compatible with multiple instruments and facilitates a wide range of applications requiring nucleic acid delivery to cells. The Ingenio® solution is available alone and as part of a complete kit with cuvettes and cell droppers.
Ingenio® Electroporation Kit

Ingenio® Electroporation Kit

High Efficiency Plasmid DNA Electroporation of Human Induced Pluripotent Stem (iPS) Cells using Ingenio®

High Efficiency Plasmid DNA Electroporation of Human Induced Pluripotent Stem (iPS) Cells using Ingenio®.
The Ingenio® Electroporation Kit was used to transfect 2 x 106 iPS cells on the Amaxa® Nucleofector® II Device. Cells were electroporated with 8 µg ZsGreen expressing plasmid (Clontech) in 100 µl and plated in 6-well plates at 0.33 x 106 cells/well. Cells were visualized 24 hours post-transfection and imaged under 4X objective with an Olympus IX71® Inverted Microscope. Images are (A) phase contrast, and (B) green fluorescence. Cells were also assayed 24 hours post-transfection on an Accuri® Cytometer. The histogram (C) shows unelectroporated cells (black line) compared to cells electroporated with plasmid using the Ingenio® Electroporation Kit (green line).

Efficient Plasmid DNA Delivery Using Ingenio® Solution with Amaxa® Nucleofector® Device

Efficient Plasmid DNA Delivery Using Ingenio® Solution with Amaxa® Nucleofector® Device.
Ingenio® Electroporation Kits were used to transfect indicated cell types using Amaxa® Nucleofector® II Device. Cells were assayed at 24 hours by flow cytometry and reported as percentage of live cell population. See Ingenio® Pulse Condition Recommendations PDF

Ingenio® Solution Provides Comparable Efficiency on Amaxa® Nucleofector® Device

Ingenio® Solution Provides Comparable Efficiency on Amaxa® Nucleofector® Device.
Cells were electroporated in parallel with an EGFP reporter vector and assayed at 24 hours post-electroporation by flow cytometry. Two electroporators were used with different electroporation kits: the Ingenio® Electroporation Kit was used in the Gene Pulser® Xcell™ Eukaryotic System (Bio-Rad®) and the Amaxa® Nucleofector® II Device (Lonza); the Amaxa® Nucleofector® Kit V was used in the Amaxa® Nucleofector® II Device, all according to manufacturer's recommendations.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.

Ingenio® Electroporation Solution and Kits

Order# Description Amount Price Data Sheet
MIR50111 Ingenio® Electroporation Solution 25 reactions 150,00 PDF
MIR50114 Ingenio® Electroporation Solution 50 reactions 239,00 PDF
MIR50117 Ingenio® Electroporation Solution 100 reactions 416,00 PDF
MIR50113 Ingenio® Electroporation Kits for all conventional electroporators, such as Bio-Rad® and Harvard-BTX® (0.4 cm cuvettes and cell droppers) 25 reactions 239,00 PDF
MIR50116 Ingenio® Electroporation Kits for all conventional electroporators, such as Bio-Rad® and Harvard-BTX® (0.4 cm cuvettes and cell droppers) 50 reactions 416,00 PDF
MIR50119 Ingenio® Electroporation Kits for all conventional electroporators, such as Bio-Rad® and Harvard-BTX® (0.4 cm cuvettes and cell droppers) 100 reactions 772,00 PDF
MIR50112 Ingenio® Electroporation Kit for Lonza-Amaxa® Nucleofector® II/2b devices (0.2 cm cuvettes and cell droppers) 25 reactions 239,00 PDF
MIR50115 Ingenio® Electroporation Kit for Lonza-Amaxa® Nucleofector® II/2b devices (0.2 cm cuvettes and cell droppers) 50 reactions 416,00 PDF
MIR50118 Ingenio® Electroporation Kit for Lonza-Amaxa® Nucleofector® II/2b devices (0.2 cm cuvettes and cell droppers) 100 reactions 772,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Ingenio® Electroporation Accessories

Order# Description Amount Price Data Sheet
MIR50120 Ingenio® Cuvettes for Lonza-Amaxa® Nucleofector® II/2b devices 25 pk, 0.2 cm cuvettes 98,00 PDF
MIR50121 Ingenio® Cuvettes for Lonza-Amaxa® Nucleofector® II/2b devices 50 pk, 0.2 cm cuvettes 193,00 PDF
MIR50123 Ingenio® Cuvettes for all conventional electroporators, such as Bio-Rad® and Harvard-BTX® 50 pk, 0.4 cm cuvettes 193,00 PDF
MIR50125 Ingenio® Cell Droppers 50 Cell droppers 54,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

  • * Sample Policy:
    MoBiTec together with Mirus considers sample requests for up to three different transfection products and one electroporation product per laboratory. Please include all samples to be tested in a single request.

Download

  • Ingenio® Electroporation Kits & Solution Flyer PDF
  • Electroporation of Human Induced Pluripotent Stem Cells (hiPSCs) PDF
  • Ingenio® Pulse Condition Recommendations PDF
  • Electroporating Primary Cells and Other Hard to Transfect Cell Types using Ingenio® Electroporation Solution PDF