Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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MiraCLEAN® Endotoxin Removal Kit

A convenient and effective method for the removal of endotoxin from plasmid DNA preparations

Endotoxin Content in Plasmid DNA after Treatment with the MiraCLEAN® Kit

The MiraCLEAN® Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations. Plasmid DNA was spiked with E. coli endotoxin at 50,000 endotoxin units per mg of DNA (EU/mg). The DNA was treated with the MiraCLEAN® Kit using three rounds of extraction. Endotoxin levels were decreased by over 2,000 fold.

Plasmid DNA Exhibits High Transfection Efficiency after Treatment with the MiraCLEAN® Kit

Plasmid DNA Exhibits High Transfection Efficiency after Treatment with the MiraCLEAN® Kit. COS-7 cells were transfected with 2 µg of plasmid DNA and 2 µl of the TransIT-LT1 Reagent in 6-well plates. Cells were harvested at 24 hours post transfection and assayed for luciferase expression.

Features

  • Effectively Removes Endotoxin from Plasmid Samples - Aids in the safety and efficiency of gene delivery research.
  • Compatible With in vivo and in vitro Applications - Removes harmful endotoxin that could cause decreased transfection efficiencies in vitro and inflammatory reactions in vivo.
  • Ease of Use - Simple phase separation protocol allows quick visualization of recovered nucleic acid preparations.

Description

  • Many molecular biology laboratory applications require endotoxin-free preparations of plasmid DNA and high molecular weight genomic DNA. The MiraCLEAN® Endotoxin Removal Kit provides a convenient and improved method for the removal of bacterial endotoxins from DNA for both in vivo and in vitro applications. E. coli, a Gram-negative eubacteria, is the common host for plasmid production. The outer leaflet of the outer membrane contains lipopolysaccharides (endotoxin) which can cause inflammatory reactions, fever and endotoxic shock in vivo. Endotoxin in plasmid preparations is also known to decrease transfection efficiencies in vitro. The MiraCLEAN® Endotoxin Removal Kit is based on a rapid phase extraction that efficiently and conveniently removes endotoxin contamination from DNA. The proprietary pink-colored EndoGO Extraction Reagent allows better visualization of the interface thereby facilitating a phase separation and increasing the recovery of nucleic acid.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR5910 MiraCLEAN® Endotoxin Removal Kit for 10 mg DNA 168,00 PDF
MIR5900 MiraCLEAN® Endotoxin Removal Kit for 100 mg DNA 494,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.