Minute™ Single Cell Isolation Kit
MinuteTM Single Cell Isolation Kit (order# SC-012)
Single cell# per 50 mg fresh mouse tissueand viability:
|Cell # in Millions||4-5||5-6||5-6||6-7||15-16|
Single cell# per 50 mg formaldehyde fixed mouse tissue:
|Cell # in Millions||4-5||4-5||4-5||5-6||13-15|
- Invent Biotechnologies' Minute™ single cell isolation kit is composed of optimized tissue disaggregation buffers and specially designed filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate single cells/nuclei from fresh/frozen/formaldehyde fixed animal tissues. The tissue disaggregation buffers are formulated to gently disaggregate animal tissues. The buffers do not contain any proteinases that may have an advert effect on cell surface marker detection. Due to the use of filter cartridges with pre-defined pore size and thickness, single cell suspension can be isolated from fresh or fixed tissues quickly with high yield and viability. Single cells isolated with the kit can be used for chromosome immunoprecipitation (ChIP) and FACS analysis. The single cell suspension can also be used as a starting material for isolation/purification of DNA, RNA, proteins and other cellular components.
- Single cells isolated with this kit can be used as starting materials for chromosome immunoprecipitation (ChIP) and FACS analysis. The single cell suspension can also be used as a starting material for isolation/purification of DNA, RNA, proteins and other cellular components.
|Buffer A (for non-fixed tissues)||25 ml||SC-012-N|
|Buffer B (for fixed tissues)||25 ml||SC-012-F|
|Cell isolation filter cartridges||50||P-001|
|Collection tubes with cap||50||P-002|