Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Cell Isolation

Minute™ Single Cell Isolation Kit

Minute Single Cell Isolation Kit

MinuteTM Single Cell Isolation Kit (order# SC-012)

Images of Isolated Single Cells from Fresh Mouse Tissues

Single cell# per 50 mg fresh mouse tissueand viability:

Tissues Brain Kidney Liver Lung Spleen
Cell # in Millions 4-5 5-6 5-6 6-7 15-16
Viability (%) 40-50 50-55 70-75 70-80 90-95
Images of Single Cells Isolated from Formaldehyde fixed Mouse Tissues

Single cell# per 50 mg formaldehyde fixed mouse tissue:

Tissues Brain Kidney Liver Lung Spleen
Cell # in Millions 4-5 4-5 4-5 5-6 13-15

Description

  • Invent Biotechnologies' Minute™ single cell isolation kit is composed of optimized tissue disaggregation buffers and specially designed filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate single cells/nuclei from fresh/frozen/formaldehyde fixed animal tissues. The tissue disaggregation buffers are formulated to gently disaggregate animal tissues. The buffers do not contain any proteinases that may have an advert effect on cell surface marker detection. Due to the use of filter cartridges with pre-defined pore size and thickness, single cell suspension can be isolated from fresh or fixed tissues quickly with high yield and viability. Single cells isolated with the kit can be used for chromosome immunoprecipitation (ChIP) and FACS analysis. The single cell suspension can also be used as a starting material for isolation/purification of DNA, RNA, proteins and other cellular components.

Application

  • Single cells isolated with this kit can be used as starting materials for chromosome immunoprecipitation (ChIP) and FACS analysis. The single cell suspension can also be used as a starting material for isolation/purification of DNA, RNA, proteins and other cellular components.

Kit Components:

Description Amount Part #
Buffer A (for non-fixed tissues) 25 ml SC-012-N
Buffer B (for fixed tissues) 25 ml SC-012-F
Cell isolation filter cartridges 50 P-001
Collection tubes with cap 50 P-002
Plastic rods 4 P-003

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
SC-012-1 PDF
SC-012-2 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe. For further pricing and order information please ask your local distributor.