Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Stem Cell Transfection Solutions

High Efficiency Transfection of Stem Cells

Stem Cells
Stem Cell Transfection

Ideal Entry Points for Transfection in Stem Cell Workflow.
Somatic cells such as adult fibroblast cells can be transfected or transduced via several methods (e.g. recombinant virus, plasmid, protein, mRNA, small molecule and miRNA) with a combination of transcription factors including KLF4, SOX2, c-Myc, Nanog, Oct-3/4 and LIN-28 to reprogram the cells to a pluripotent state. iPS cells can then be differentiated to a myriad of cell types through growth factor addition and/or transfection of selection markers driven by cell type specific promoters. Stem cell derived cell types such as cardiomyocytes, adipocytes, neural cells, pancreatic-ß cells, and hematopoietic progenitor cells can provide researchers with relevant models for their experiments.

Features

  • Transfect DNA effectively - with TransIT®-2020 or TransIT®-LT1
  • Perform repeated mRNA transfections - using TransIT®-mRNA Transfection Kit
  • Introduce siRNA - with TransIT-TKO® or TransIT-siQUEST®
  • Electroporate stem cells efficiently and cost-effectively - with Ingenio® Electroporation Solution

Why Stem Cells?

  • Advances in the field of stem cell differentiation and reprogramming have accelerated drug development in recent years by providing homogenous cell populations for cell-based testing. Several of these breakthroughs rely on the use of transfection for non-viral delivery of nucleic acids into different cell types. Mirus Bio provides high efficiency nucleic acid delivery tools through a suite of TransIT® Transfection Reagents and the Ingenio® Electroporation Kits that have been validated for many of these applications.

The Role of Transfection in Stem Cell Applications

Primary and Stem Cells Transfected using Mirus Bio Reagents

Cell Type Nucleic Acid Mirus Reagent(s) Used Application
Human embryonic stem cell derived neural progenitors Plasmid DNA TransIT®-2020 Stem cell transfection
Human foreskin fibroblasts Plasmid DNA TransIT®-2020 DNA transfection
Human induced pluripotent stem (iPS) cells Plasmid DNA TransIT®-2020 Stem cell transfection
Human induced pluripotent stem (iPS) cells Plasmid DNA Ingenio® Stem cell transfection
Human mesenchymal stem cells Plasmid DNA TransIT®-2020 Stem cell transfection
Human skin fibroblasts Plasmid DNA TransIT®-2020 DNA transfection
iCell® Cardiomyocytes Plasmid DNA TransIT®-LT1 Stem cell transfection
Mouse embryonic fibroblasts Plasmid DNA TransIT®-2020 DNA transfection
Mouse embryonic stem cell derived cardiomyocytes Plasmid DNA TransIT®-2020 Stem cell transfection
Normal human dermal fibroblasts (NHDF) Plasmid DNA TransIT®-2020 DNA transfection
SRF -/- mouse embryonic stem cells Plasmid DNA TransIT®-LT1 Stem cell transfection
BJ human neonatal foreskin fibroblasts mRNA TransIT®-mRNA Stem cell reprogramming
C3H/10T1/2 mRNA TransIT®-mRNA Stem cell reprogramming
CCD-1109Sk human normal adult skin fibroblasts mRNA TransIT®-mRNA Stem cell reprogramming
MRC-5 human lung fibroblasts mRNA TransIT®-mRNA Stem cell reprogramming
Primary human neonatal epidermal keratinocytes mRNA TransIT®-mRNA Stem cell reprogramming
Primary human lung fibroblasts mRNA TransIT®-mRNA Stem cell reprogramming
Human mesenchymal stem cells siRNA TransIT-TKO® Stem cell differentiation
iCell® Cardiomyocytes siRNA TransIT-TKO® Knockdown of gene expression
Human adipose derived adult stem cells (hADAS) siRNA TransIT-siQUEST® Knockdown of gene expression

Prove It to Yourself! Request a FREE Sample for Your Stem Cell Transfections

mir-5404

FREE Sample

mir-2304

FREE Sample

mir-2225

FREE Sample

mir-2150

FREE Sample

mir-50112

FREE Sample

TransIT®-2020 Transfection Reagent

  • Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
  • Gentle to Your Cells – Balances high efficiency nucleic acid delivery and low cellular toxicity
  • Animal Origin Free – High performance with maximum compatibility

TransIT®-LT1 Transfection Reagent

  • Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases
  • High Efficiency Delivery – Achieve expression in a large population of cells

TransIT®-mRNA Transfection Kit

  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases
  • High Efficiency Delivery – Achieve RNA delivery in a large population of cells
  • Serum Compatible – Perform transfections in the presence of serum which eliminates the need for a media change

TransIT-TKO® Transfection Reagent

  • Broad Spectrum siRNA and Plasmid DNA Delivery –Utilize one transfection reagent for a wide variety of cells
  • Low Cellular Toxicity –Maintain cell density and reduce experimental biases
  • Reproducible Results – Obtain consistent, targeted gene knockdowns from day to day
  • High Knockdown Efficiency –Achieve optimal gene silencing in a large percentage of cells to ensure experimental success

Ingenio® Electroporation Solution

  • High Efficiency Electroporation – Deliver to hard to transfect cell lines and primary cells
  • Compatible with Most Conventional Electroporation Devices – Use your existing system including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®
  • Save Money Without Sacrificing Performance – Available as a stand-alone solution or as part of a complete kit with cuvettes and cell droppers

Sample Policy

  • MoBiTec together with Mirus considers sample requests for up to three different transfection products and one electroporation product per laboratory. Please include all samples to be tested in a single request.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR5404 TransIT®-2020 Transfection Reagent 0.4 ml 217,00 PDF
MIR5400 TransIT®-2020 Transfection Reagent 1 ml 429,00 PDF
MIR5405 TransIT®-2020 Transfection Reagent 5 x 1 ml 1869,00 PDF
MIR5406 TransIT®-2020 Transfection Reagent 10 x 1 ml 3455,00 PDF
MIR2304 TransIT®-LT1 Transfection Reagent 0.4 ml 200,00 PDF
MIR2300 TransIT®-LT1 Transfection Reagent 1 ml 317,00 PDF
MIR2305 TransIT®-LT1 Transfection Reagent 5 x 1 ml 1372,00 PDF
MIR2306 TransIT®-LT1 Transfection Reagent 10 x 1 ml 2536,00 PDF
MIR2225 TransIT®-mRNA Transfection Kit 0.4 ml 362,00 PDF
MIR2250 TransIT®-mRNA Transfection Kit 1 ml 647,00 PDF
MIR2255 TransIT®-mRNA Transfection Kit 5 x 1 ml 2834,00 PDF
MIR2256 TransIT®-mRNA Transfection Kit 10 x 1 ml 5243,00 PDF
MIR2154 TransIT-TKO® Transfection Reagent 0.4 ml 276,00 PDF
MIR2150 TransIT-TKO® Transfection Reagent 1.5 ml 442,00 PDF
MIR2155 TransIT-TKO® Transfection Reagent 5 x 1.5 ml 1920,00 PDF
MIR2156 TransIT-TKO® Transfection Reagent 10 x 1.5 ml 3549,00 PDF
MIR50111 Ingenio® Electroporation Solution 25 reactions 150,00 PDF
MIR50114 Ingenio® Electroporation Solution 50 reactions 239,00 PDF
MIR50117 Ingenio® Electroporation Solution 100 reactions 416,00 PDF
MIR50113 Ingenio® Electroporation Kits for all conventional electroporators, such as Bio-Rad® and Harvard-BTX® (0.4 cm cuvettes and cell droppers) 25 reactions 239,00 PDF
MIR50116 Ingenio® Electroporation Kits for all conventional electroporators, such as Bio-Rad® and Harvard-BTX® (0.4 cm cuvettes and cell droppers) 50 reactions 416,00 PDF
MIR50119 Ingenio® Electroporation Kits for all conventional electroporators, such as Bio-Rad® and Harvard-BTX® (0.4 cm cuvettes and cell droppers) 100 reactions 772,00 PDF
MIR50112 Ingenio® Electroporation Kit for Lonza-Amaxa® Nucleofector® II/2b devices (0.2 cm cuvettes and cell droppers) 25 reactions 239,00 PDF
MIR50115 Ingenio® Electroporation Kit for Lonza-Amaxa® Nucleofector® II/2b devices (0.2 cm cuvettes and cell droppers) 50 reactions 416,00 PDF
MIR50118 Ingenio® Electroporation Kit for Lonza-Amaxa® Nucleofector® II/2b devices (0.2 cm cuvettes and cell droppers) 100 reactions 772,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

  • * Sample Policy:
    MoBiTec together with Mirus considers sample requests for up to three different transfection products and one electroporation product per laboratory. Please include all samples to be tested in a single request.

Download

  • Mirus Stem Cell Brochure (PDF)
  • Mirus High Efficiency Transfection of iCell® Cardiomyocytes and Stem Cell Relevant Cell Sources (PDF)
  • Mirus Electroporation of Human Induced Pluripotent Stem Cells with Ingenio Electroporation Solution (PDF)
  • Mirus Reverse Transfection of Human Induced Pluripotent Stem Cells with TransIT®-LT1 Transfection Reagent (PDF)