Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology


TransIT-X2® Dynamic Delivery System

An Advanced System for Delivery of Plasmid DNA, siRNA/miRNA and CRISPR/Cas9 Components


  • Efficiency – Exceptional Broad Spectrum Transfection
  • Versatility – Cutting Edge Delivery of Plasmid DNA and small RNAs (siRNA, miRNA and CRISPR guide RNA)
  • Technology – Novel. Non-Liposomal. Polymeric Delivery


Experience the transfection breakthrough. Achieve superior transfections with an innovative polymeric system that efficiently delivers both DNA and RNA out of the endosome and into the cytoplasm overcoming a critical barrier to nucleic acid delivery.

    TransIT-X2® Dynamic Delivery System is an advanced, non-liposomal polymeric system that enables high efficiency transfection of many cell types, including primary cells. TransIT-X2® can be used for DNA delivery, siRNA delivery or simultaneous delivery of DNA and siRNA. Transfections with TransIT-X2® do not require medium changes and can be carried out in serum-containing medium.

TransIT-X2® System Enables Superior Gene Expression in a Variety of Cell Types

TransIT-X2® Dynamic Delivery System and Lipofectamine® 2000 Transfection Reagent were used to transfect plasmid DNA encoding luciferase into 41 different cell types at three reagent-to-DNA ratios. Luciferase expression was compared at 24 hours post-transfection using a standard luciferase assay. Head-to-head comparisons at optimized ratios illustrate superior or equal luciferase expression using TransIT-X2® in 36 of 41 cell types; 17 cell types that had expression levels 2-fold higher are denoted with ‡.

TransIT-X2® Dynamic Delivery System

Experimental Data

Comparison of TransIT-X2® Dynamic Delivery System with Lipofectamine® 2000

TransIT-X2® Dynamic Delivery System Achieves Higher Knockdown than Lipofectamine® 2000.

Knockdown of PTK9 mRNA with TransIT-X2® Dynamic Delivery System

Effective miRNA Delivery usingTransIT-X2® Dynamic Delivery System Yields Decreased Levels of PTK9 mRNA.

The Simplicity of Transfection
A clear and simple illustration of the process of transfection.


  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR6000 TransIT-X2® Dynamic Delivery System 1.5 ml 557,00 PDF
MIR6003 TransIT-X2® Dynamic Delivery System 0.3 ml 134,00 PDF
MIR6004 TransIT-X2® Dynamic Delivery System 0.75 ml 335,00 PDF
MIR6005 TransIT-X2® Dynamic Delivery System 5 x 1.5 ml 2421,00 PDF
MIR6006 TransIT-X2® Dynamic Delivery System 10 x 1.5 ml 4452,00 PDF
MIR6010 TransIT-X2® Dynamic Delivery System 0.1 ml 0,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

  • * Sample Policy:
    MoBiTec together with Mirus considers sample requests for up to three different transfection products and one electroporation product per laboratory. Please include all samples to be tested in a single request.


  • CRISPR-Cas9-Poster
  • Mirus CRISPR/Cas9 Transfection Poster (PDF):
    Optimization of DNA, RNA and RNP Delivery Methods for Efficient CRISPR/Cas9 Mediated Mammalian Cell Engineering
  • Mirus White Paper (PDF):
    Optimization of DNA, RNA and RNP Delivery for Efficient Mammalian Cell Engineering Using CRISPR/Cas9
  • Cell-type specific transfection protocol recommendations PDF
  • TransIT-X2™ Dynamic Delivery System protocol PDF
  • TransIT-X2™ Dynamic Delivery System MSDS PDF