Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

TransIT-X2® Dynamic Delivery System

An Advanced System for Delivery of Plasmid DNA, siRNA/miRNA and CRISPR/Cas9 Components

Features

  • Efficiency – Exceptional Broad Spectrum Transfection
  • Versatility – Cutting Edge Delivery of Plasmid DNA and small RNAs (siRNA, miRNA and CRISPR guide RNA)
  • Technology – Novel. Non-Liposomal. Polymeric Delivery

Description

Experience the transfection breakthrough. Achieve superior transfections with an innovative polymeric system that efficiently delivers both DNA and RNA out of the endosome and into the cytoplasm overcoming a critical barrier to nucleic acid delivery.

    TransIT-X2® Dynamic Delivery System is an advanced, non-liposomal polymeric system that enables high efficiency transfection of many cell types, including primary cells. TransIT-X2® can be used for DNA delivery, siRNA delivery or simultaneous delivery of DNA and siRNA. Transfections with TransIT-X2® do not require medium changes and can be carried out in serum-containing medium.

TransIT-X2® System Enables Superior Gene Expression in a Variety of Cell Types

TransIT-X2® Dynamic Delivery System and Lipofectamine® 2000 Transfection Reagent were used to transfect plasmid DNA encoding luciferase into 41 different cell types at three reagent-to-DNA ratios. Luciferase expression was compared at 24 hours post-transfection using a standard luciferase assay. Head-to-head comparisons at optimized ratios illustrate superior or equal luciferase expression using TransIT-X2® in 36 of 41 cell types; 17 cell types that had expression levels 2-fold higher are denoted with ‡.

TransIT-X2® Dynamic Delivery System

Experimental Data

Comparison of TransIT-X2® Dynamic Delivery System with Lipofectamine® 2000

TransIT-X2® Dynamic Delivery System Achieves Higher Knockdown than Lipofectamine® 2000.

Knockdown of PTK9 mRNA with TransIT-X2® Dynamic Delivery System

Effective miRNA Delivery usingTransIT-X2® Dynamic Delivery System Yields Decreased Levels of PTK9 mRNA.

The Simplicity of Transfection
A clear and simple illustration of the process of transfection.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR6000 TransIT-X2® Dynamic Delivery System 1.5 ml 547,00 PDF
MIR6003 TransIT-X2® Dynamic Delivery System 0.3 ml 132,00 PDF
MIR6004 TransIT-X2® Dynamic Delivery System 0.75 ml 329,00 PDF
MIR6005 TransIT-X2® Dynamic Delivery System 5 x 1.5 ml 2374,00 PDF
MIR6006 TransIT-X2® Dynamic Delivery System 10 x 1.5 ml 4365,00 PDF
MIR6010 TransIT-X2® Dynamic Delivery System 0.1 ml 0,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

  • * Sample Policy:
    MoBiTec together with Mirus considers sample requests for up to three different transfection products and one electroporation product per laboratory. Please include all samples to be tested in a single request.

Download

  • CRISPR-Cas9-Poster
  • Mirus CRISPR/Cas9 Transfection Poster (PDF):
    Optimization of DNA, RNA and RNP Delivery Methods for Efficient CRISPR/Cas9 Mediated Mammalian Cell Engineering
  • Mirus White Paper (PDF):
    Optimization of DNA, RNA and RNP Delivery for Efficient Mammalian Cell Engineering Using CRISPR/Cas9
  • Cell-type specific transfection protocol recommendations PDF
  • TransIT-X2™ Dynamic Delivery System protocol PDF
  • TransIT-X2™ Dynamic Delivery System MSDS PDF