High Yield Transient Transfection and Baculovirus Titers in Insect Cells
Efficient Transfection of Baculovirus Genomic DNA using TransIT®-Insect Reagent.
Transfections were performed in 6-well plates with 5 x 105 Sf9 cells per well using TransIT®-Insect Transfection Reagent at a reagent-to-total DNA ratio of 3:1 (µl:µg). Cells were co-transfected with 0.5 µg of ProGreen™ baculovirus genomic vector DNA (AB Vector) encoding green-fluorescent protein (GFP) and 0.1 µg of pVL1393 transfer vector (AB Vector). (A) Fluorescence image was taken at 6 days post-transfection using a Zeiss S100 fluorescent microscope. Merger with phase contrast image shown in (B).
TransIT®-Insect Outperforms Competitor Transfection Reagents.
Insect cell lines (A) Sf9, (B) High Five™, and (C) Drosophila S2 cells were transfected in 96-well plates with 0.1 µg of a luciferase expression plasmid driven by an hr5 enhancer/IE1 promoter using the designated reagent at the indicated reagent-to-DNA ratios (µl:µg). Luciferase expression was measured at 48 hours post-transfection. Sf9 and High Five™ cells were cultured and transfected in serum-free media formulations; S2 cells were in serum containing medium. Error bars represent the standard error of the mean for triplicate wells.
TransIT®-Insect Yields Increased Protein Expression Over Time.
Insect cell lines (A) Sf9, (B) High Five™, and (C) Drosophila S2 were transfected in a 96-well plate with 0.1 ug of a luciferase expression plasmid driven by an hr5 enhancer/IE1 promoter using the TransIT®-Insect Transfection Reagent at a reagent-to-DNA ratio of 2:1 (µl:µg). Luciferase expression was measured at three time points: 24, 48 and 72 hours post-transfection. Sf9 and High Five™ cells were cultured and transfected in serum-free media formulations; S2 cells were in serum containing medium. Error bars represent the standard error of the mean for triplicate wells.
- Effective DNA Delivery - In insect cell types including Sf9, High Five™, and S2
- High Baculovirus Production - Ideal for baculovirus expression in insect cells
- Serum Compatibility - Non-liposomal animal-origin free formulation that eliminates media change
- Better Value - Low reagent amounts required per transfection
- Insect cell expression is a platform used to produce proteins with simple post-translational modifications. Transient transfection and recombinant baculovirus production are commonly used methods for insect cell expression. TransIT®-Insect Transfection Reagent is an animal-origin free transfection reagent specifically optimized for high gene expression in a variety of insect cell types.
- For shipping and storage information please click on Order#.
|MIR6100||TransIT®-Insect Transfection Reagent||1 ml||255,00|
|MIR6104||TransIT®-Insect Transfection Reagent||0.4 ml||123,00|
|MIR6105||TransIT®-Insect Transfection Reagent||5 x 1 ml||1104,00|
|MIR6106||TransIT®-Insect Transfection Reagent||10 x 1 ml||2028,00|
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- * Sample Policy:
MoBiTec together with Mirus considers sample requests for up to three different transfection products and one electroporation product per laboratory. Please include all samples to be tested in a single request.