Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Immobilized Enzymes

Immobilized Enzymes for Solid Phase Enzyme Reactions

Using CRC columns saves time

Using our immobilized enzymes in compact reaction columns (CRC) saves your time in many molecular biological applications.

Features

  • Supplied in compact reaction columns (CRC)
  • High enzyme concentration and activity
  • Skip extensive steps in lab protocols
  • Convenience and ease of handling
  • Omit phenol extractions
  • Avoid contaminating the lab (and your reaction solutions) with enzymes (RNase!)
  • Enjoy short reaction and treatment times
  • Get absolutely clean solutions
  • Reusability - use CR columns over and over again
  • Take CR columns even for tiny solution volumes (20 µl!)
  • Retain small volumes even after treatment
  • Treat larger volumes using syringes or tubing with standard Luer-lock connections

Description

  • Compact reaction columns are small volume columns (Mobicols) containing a matrix to which enzymes are bound (immobilized covalently). Enzyme reactions occur when the substrate is added to the column. The sample is recovered from the column quantitatively by washing or centrifugation. The enzyme remains bound to the column.
  • Our Immobilization Technology was developed in co-operation with the Max Planck Society and is patented. It enables us to offer enzymes immobilized on both polyvinyl and dextran matrix. The immobilized enzymes retain their full activity and possess an extremely high occupational density.
  • Immobilized enzymes are supplied in extremely versatile compact reaction columns (CRC) which fit into 1.5 ml microcentrifuge tubes. The columns have Luer-lock fittings, allowing direct syringe application of substrate solution, continuous flow processing of bulk solutions, or application of pressure for recovering the substrate. For small substrate volumes (approx. 50 µl or less), most enzyme columns can be spun dry in benchtop centrifuges for quick, effective recovery.
  • The immobilized enzymes are extremely stable in aqueous media over a pH range of 5 to 10 and column bleeding is negligible. The "stiff" linkers which separate the enzyme from the matrix surface effectively eliminate steric hindrance. As a result, the enzymes retain essentially their full activity in the immobilized state.
  • This well established technology allows you to expose your reaction solutions to very high concentrations of modifying enzymes. The exposure to high enzyme concentrations allows reaction times to be greatly reduced.

Two different immobilization matrices are available:

Matrix F7mMatrix G3m
Matrix F7m has large pores. Molecules with up to 107 Dalton molecular weight (most enzymes and substrates) can enter these pores. The total surface of the material is very large, resulting in an extremely high enzyme activity on the matrix. G3m has extremely small pores; this results in excellent recovery characteristics (i.e., complete recovery in very small volumes). Molecules larger than 103 Dalton molecular weight (larger peptides, proteins, and nucleic acids) cannot enter these pores. The total surface area of the material is smaller than that of F7m resulting in a smaller enzyme activity on the matrix.

ORDER INFORMATION        Limited stock due to discontinuation of immobilized enzymes!

  • For shipping and storage information please click on Order#.

Immobilized Proteases (in CRC)

Order# Description Amount Price Data Sheet
P5101 Endoproteinase Glu-C (Prot.V8)(one Column with 200 µl matrix F7m) 900 U 308,00 PDF
P3102 Endoproteinase Glu-C (Prot.V8)(one Column with 200 µl matrix G3m) 22 U 182,00 PDF
P3402 Papain (one Column with 200 µl matrix G3m) 0.6 U 148,00 PDF
P5121 Pepsin (one Column with 200 µl matrix F7m) 16mAnson U 182,00 PDF
P3122 Pepsin (one Column with 200 µl matrix G3m) 0.4mAnson U 148,00 PDF
P3502 Proteinase K (one Column with 200 µl matrix G3m) 0.7mAnson U 148,00 PDF
P5301 TLCK-Chymotrypsin (one Column with 200 µl matrix F7m) 55 U 182,00 PDF
P3302 TLCK-Chymotrypsin (one Column with 200 µl matrix G3m) 1.4 U 148,00 PDF
P5701 TPCK-Trypsin (one Column with 200 µl matrix F7m) 10.200 St-U 182,00 PDF
P3702 TPCK-Trypsin (one Column with 200 µl matrix G3m) 260 St-U 148,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

Immobilized Nucleases (in CRC)

Order# Description Amount Price Data Sheet
N3102 RNase A (one Column with 200 µl matrix G3m) 2.5 Kunitz-U 148,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

Immobilized Other Enzymes (in CRC)

Order# Description Amount Price Data Sheet
A3202 Alkaline Phosphatase (CIP) (one Column with 200 µl matrix G3m) 100 U 134,00 PDF
A3102 ß-Galactosidase (one Column with 200 µl matrix G3m) 15 U 134,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

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