Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Nucleic Acid Purification

AquaPreserve™

Preserve and extract DNA, RNA and proteins without crosslinking

Gross anatomy of liver tissues preserved in AquaPreserve™ and PBS

Gross anatomy of liver tissues preserved in AquaPreserve™ and PBS. Fresh mouse liver tissues (~100 mg) were submerged in 0.2 ml AquaPreserve™ (left) or PBS (right) and stored at 22 °C for 7 days. As seen, liver tissues preserved in AquaPreserve™ looked fresh, remained intact, and wedged in the tube, while liver tissues stored in PBS became highly degraded and sank to the bottom of the tube.

Features

  • Preservation and extraction
  • Stabilization without crosslinking
  • Preservation without freezing
  • Extracting DNA, RNA, and proteins from tissue and fresh or frozen blood

Description

  • AquaPreserve™ is a multifunctional reagent for DNA/RNA/protein preservation and extraction. It may be used to streamline biospecimen collection, stabilization, transport, storage, distribution, and DNA/RNA/protein extraction. By streamlining the entire biospecimen workflow, AquaPreserve can reduce pre-analytical variability, increase data reproducibility and reliability. AquaPreserve extracts total DNA/RNA/proteins from whole blood; it recovers both cellular and cell-free circulating DNA/RNA/proteins in the whole blood, therefore, maximizing the scientific value and utilities of the biospecimens. AquaPreserve is the only reagent that can extract intact RNA from frozen whole blood samples collected in common anticoagulants.
  • Streamline biospecimen pre-analytical workflow:
  • AquaPreserve not only preserves but also extracts DNA/RNA/proteins from the biospecimen. It can be used to streamline the entire pre-analytical workflow, from specimen collection (immediately arrests gene expression and stabilizes the DNA/RNA/proteins) to DNA/RNA/protein extraction (does not use any other DNA, RNA, and protein extraction kits). Therefore, it reduces biospecimen pre-analytical variability, prevents sample cross-contamination, increases detection specificity, and improves data reproducibility and reliability.
  • Safeguard biospecimens during their transportation and storage:
  • AquaPreserve-stabilized specimens may be stored at 4 and 22 °C for weeks without causing RNA degradation. AquaPreserve serves a safeguard against unexpected cold-chain disasters during shipping and storage, and makes it feasible to repeatedly thaw the frozen the specimens for QC/QA and for distribution to researchers.
  • Maximize the scientific value of the biospecimens:
  • Presently you probably only store cellular DNA extracted from fresh blood and you don't have a reliable method to extract RNA from frozen whole blood collected in common anticoagulants (not in specialized PAXgene or Tempus RNA tubes). With AquaPreserve, you not only can extract blood DNA but also blood RNA, not only cellular DNA/RNA/proteins but also cell-free circulating DNA/RNA/proteins, from not only fresh but also archived frozen whole blood. It allows you to do more researches and generate more data previously impossible from your precious specimens.
  • Reduce the overall cost of biobanking:
  • AquaPreserve enables the freezing of leftover blood samples obtained from routine testing for later DNA/RNA/protein extraction, therefore, opens up an unlimited and inexpensive biospecimen resource. AquaPreserve-stabilized the blood biospecimens may be shipped and stored at 4 or 22 °C for years without causing DNA degradation, therefore, reducing the energy consumption. Additionally, AquaPreserve extracts DNA/RNA/proteins from the specimens without needing other DNA, RNA, and protein extraction kits.

AquaPreserve™ is scalable.

Use a volume ratio of 1:1:0.5 (blood:AquaPreserve:ProSink) for extraction:

Micro Mini Midi Maxi
Blood 50 µl 250 µl 2 ml 4 ml
AquaPreserve 50 µl 250 µl 2 ml 4 ml
ProSink 25 µl 125 µl 1 ml 2 ml
Centrifuge tubes 0.6 ml 1.5 ml 15 ml 15 ml
DNA yield 2-3 µg 12-15 µg 100-130 µg 200-250 µg
RNA yield 50 µg 250 µg 2 mg 4 mg
Number of extractions 1,200 240 30 15

Comparison of Techniques for Biospecimen Preservation

  Formalin R*later AquaPreserve™
Mechanism of Stabilization Cross-linking High salt dehydration Enzyme inactivation
Add to Frozen Specimens No No. Require pre-soaking Yes
Storage Temperature 22 °C 22 °C 22 to -80 °C
Damage to Specimen Yes No No
Inactivation of Pathogens Yes No Yes
Offensive Odor Yes No No
DNA, RNA, and protein Extraction No No Yes

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
8001MT AquaPreserve Trial Kit, 1 ml 4 minipreps Kit 17,00 PDF
8060MT AquaPreserve Kit, 60 ml for 240 minipreps Kit 244,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

Related Products

Order# Description Amount Price Data Sheet
9030MT ProSink 30 ml 60,00 PDF
1115MT ProMelt 15 ml 60,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.