Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Recombinant Nucleases

Recombinant Exonuclease III-T

Applications

  • Construction of nested unidirectional deletions of DNA fragments in combination with nuclease S1
  • Generation of a single-stranded template for dideoxy sequencing of DNA
  • Site-directed mutagenesis
  • Cloning of PCR products
  • In vitro recombination

Description

  • Exonuclease III (ExoIII) of E. coli is a 31 kDa monomeric, globular protein combining multiple catalytic activities in one active site. It acts on doublestranded (ds) DNA as a 3'-5' exonuclease, a 3'- phosphomonoesterase, an apurinic/apyrimidimic (AP) sites specific endonuclease and an exonucleolytic ribonuclease H. Of particular interest for molecular biological methods is its exonucleolytic activity, removing 5'-mononucleotides from the 3'-hydroxyl ends of ds DNA, leaving protruding 5'-termini. Its catalytic rate can be adjusted by temperature and NaCl concentration and thus allows for the generation of single-stranded DNA templates for sequencing or recombination methods. Recombinant Exonuclease III-T is an engineered exonuclease III with improved temperature resistance.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
GE-NUC00600-01 Recombinant Exonuclease III-T (> 10 U/uL) 20 µl 97,00 PDF
GE-NUC00600-02 Recombinant Exonuclease III-T (> 10 U/uL) 100 µl 368,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.