NOWA Chemiluminescent Western
Highly Sensitive Detection System for HRP-Conjugated Antibodies
Comparison of light emission of NOWA and popular supplier A´s kit.
- NOWA (New Optical Western Analysis) is a highly sensitive detection system for HRP-conjugated antibodies in the picogram range. It enables a sensitivity that is significantly higher than that of other leading chemiluminescence kits.
- The kit is based on the emission of light during a reaction which is started by free oxygen produced by the enzymatic activity of HRP. Using a newly developed enhancer cascade, the light emission is amplified by a factor of at least one thousand! The NOWA kit contains two solutions that are combined and used for incubating the nitrocellulose or PVDF filter. After one minute of incubation time the filter is ready to be exposed to an X-ray film. Typical exposure times range between 10 seconds and 5 minutes. The light-emitting reaction continues for about 1 hour to allow as many exposures as you may need. The shelf life of the NOWA kit is at least 12 months when stored at 4 °C. The kit contains 2 x 250 ml and is sufficient for 10,000 cm2 (1 m2 ) of NC or PVDF membrane.