Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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ATX-Red AR-2

In Vivo Imaging Probe for Visualizing and Measuring Autotaxin Activity

Features

  • Activated ATX-Red fluoresces in the near-infrared, the optimal spectral window for in vivo fluorescence imaging
  • Near-IR fluorescence can be imaged up to 15 cm deep in living tissue (Ntziachristos and Weissleder, Opt Lett. 2001)

Description

  • ATX-Red AR-2 is an in vivo imaging probe for visualizing and measuring Autotaxin (ATX) activity. It is an analog of the autotaxin substrate lysophosphatidylcholine (LPC) and contains a near-infrared fluor (Licor IRDye® 800CW) and quencher (Licor IRDye® QC-1). When autotaxin cleaves ATX-Red AR-2, fluorescence increases. This activation mechanism is specific to autotaxin in vitro and in vivo.

About Autotaxin

  • Autotaxin which has lysophospholipase D (lysoPLD) activity, cleaves choline from lysophosphatidylcholine forming lysophosphatidic acid (LPA), a potent mitogen that as been implicated in the pathophysiology of ovarian cancer. LysoPLD/ATX has been demonstrated to increase cell motility, neovascularization, proliferation and aggressiveness of tumors, and is upregulated in numerous cancer lineages (non-small cell lung, glioma, mammary carcinoma, renal cell carcinoma, hepatocellular carcinoma). In addition, dysregulation of the ATX/LPA pathway is central to the pathophysiology of idiopathic pulmonary fibrosis, rheumatoid arthritis, and other inflammatory diseases. Modulation of ATX activity through small-molecules is a currently underexplored target, but one with high potential for novel cancer and anti-inflammatory therapeutics.
Fluorescence is observed in the tumor where ATX activity is increased.

A mouse bearing an MDA-MB-231 orthotopic tumor was injected with ATX-Red AR-2 by tail vein injection and imaged at 800 nm. Fluorescence is observed in the tumor where ATX activity is increased.

ATX-Red is a smart probe for imaging autotaxin activity in vivo

ATX-Red is a smart probe for imaging autotaxin activity in vivo.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
L-2010-1-EC ATX-Red AR-2 10 ug 86,00 PDF
L-2010-2-EC ATX-Red AR-2 100 ug 666,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe except for France and Italy.

Literature

  • Ntziachristos and Weissleder, Optics Letters, Vol. 26, Issue 12, pp. 893-895 (2001) (Link)