pH Probe for Acidic-Organelle Imaging
Fluorescence Spectra of AcidiFluor™ Orange as a Function of pH
Fluorescence spectra of AcidiFluor™ Orange were measured in phosphate buffer at pH 5.0 or pH 7.4, respectively. pH 5.0 corresponds to the conditions in acidic organelles, and pH 7.4 corresponds to physiological conditions in the cytoplasm. Fluorescence intensity of AcidiFluor™ Orange at pH 5.0 is 50-fold higher than at pH 7.4.
HeLa cells expressing GFP in mitochondria were multiple stained with AcidiFluor™ ORANGE and Hoechst 33342. Lysosomes were stained orange with AcidiFluor™ ORANGE, nuclei were stained blue with Hoechst 33342 and mitochondria were stained green with GFP.
Compared to Other Dyes for Lysosome Imaging AcidiFluor™ ORANGE is Highly Resistant to Photobleaching
Samples were continuously illuminated for 180 seconds and viewed on a confocal microscope. Unlike LysoTracker® Green DND-26 and LysoTracker® Red DND-99 which discolored significantly, fluorescence of AcidiFluor™ ORANGE remained. LysoSensor™ Green DND-189 was not suitable for continuous observation because fluorescence leaked into cytoplasm during excitation light irradiation.
- pH-sensitve fluorescent probe
- Great photostability
- High sensitivity even after conjugation with proteins
- For studying endocytosis or phagocytosis
- AcidiFluor™ ORANGE is a fluorescent probe that exhibits increasing fluorescence as the pH of its surroundings becomes more acidic. This probe can be used for staining acidic organelles such as lysosomes, late endosomes, and secretory granules. Its excellent selectivity enables the detection of acidic environments. Particularly at pH 5.0 – which corresponds to pH conditions of acidic organelles – fluorescence intensity is 50-fold higher compared to fluorescence intensity at physiological pH (7.4). AcidiFluor™ ORANGE is highly resistent to bleaching. As it emits orange fluorescence after excitation, multicolor imaging is possible. For instance, a combination with blue fluorescence (CFP, Hoechst, etc.), green fluorescence (GFP, fluorescein, etc.) and near-infrared fluorescence is possible. AcidiFluor™ ORANGE is suitable for detecting acidic organelles, observing granules release, studying endocytosis/exocytosis, and much more.
Fluorescence Characteristics of AcidiFluor™ ORANGE
High Brightness and High S/N Ratio
Suitable for Multicolor Imaging
- For shipping and storage information please click on Order#.
|GC304||AcidiFluor™ORANGELabeling Kit||5 samples||697,00|
|GC305||AcidiFluor™ORANGE-Zymosan A||1 mg||497,00|
|GC306||AcidiFluor™ORANGE-Dextran 10k||1 mg||697,00|
- All prices are in EURO excl. VAT and shipping. Only available in Europe.
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- GORYO Fluorescent Probes Catalog (PDF)