Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Fluorescent Detection of Cellular Stress & Toxicity

Cell Stress Sensor Kit

Cell Stress Sensor

Detect cellular stress from: neurodegenerative diseases (ALS, Huntington's, Parkinson's), cancer, ischemia, diabetes, unfolded protein response
Detect cellular toxicity from: drug induced toxicity (chemotherapeutics), compound toxicity (Tox21 library), protein overexpression

Cell Stress Graphs

Kevin and Thom from Montana Molecular introduce the genetically encoded fluorescent sensor for cell stress / unfolded protein response.

Product Box

Features

  • Detect a wide range of cell stress; including ER and UPR
  • Bright green fluorescence response
  • Plate reader or microscope compatible
  • Live cell assay
  • Fast results

Description

  • The cell stress sensor is a genetically-encoded fluorescent biosensor that produces very bright fluorescence when the cell endures endoplasmic reticulum (ER) stress or undergoes the unfolded protein response (UPR). The biosensor is a two-color biosensor, allowing ratiometric detection of cellular stress and toxicity. The fluorescent ratio between the constitutively expressed, nuclear localized, red fluorescent protein and the stress induced, nuclear localized, green fluorescent protein is calculated to determine the level of cellular stress.
  • A broad host of both chemical compounds and genetic mutations can induce ER stress. The UPR is one of the major stress pathways within the cell, which allows the stress sensor to detect a wide range of stress inducing stimuli, some whose primary target is not the ER.

Molecular Mechanism

  • This sensor is based on the XBP1 mRNA which is spliced by the IRE1 protein during ER stress. Briefly, the model for this response involves ER stress activating the RNase activity of the IRE1 protein, which in turn splices the immature, cytosolic mRNA for XBP1, generating an active transcription factor that alters gene expression. This unique cytosolic splicing of XBP1 has been used to generate reporter systems in the past [Iwawaki et al. 2003], many of which were PCR-based splicing assays or reporters of XBP1 transcriptional activity [Rong et al. 2015]. The cell stress sensor is unique in that the assay does not rely on changes in gene expression or transcription activation. Rather, the splicing of the XBP1 intron results in translation of the bright fluorescent protein, mNeon Green. Increased translation of mNeon Green results in increased fluorescence output, indicating the occurrence of cellular stress.

Cell Stress Sensor Kit Content

  • 10, 30, or 60 mL of sensor
  • HDAC inhibitor (Sodium Butyrate)
  • Thapsigargin (positive control)

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
U0900G-10 Green Cell Stress Sensor 10 ml Kit 571,00 PDF
U0900G-30 Green Cell Stress Sensor 30 ml Kit 1379,00 PDF
U0900G-60 Green Cell Stress Sensor 60 ml Kit 2071,00 PDF
U0901G-10 Red NLS + 2A + Green Stress Sensor NLS 10 ml Kit 571,00 PDF
U0901G-30 Red NLS + 2A + Green Stress Sensor NLS 30 ml Kit 1379,00 PDF
U0901G-60 Red NLS + 2A + Green Stress Sensor NLS 60 ml Kit 2071,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe.

Download

  • Montana Molecular: Cell Stress Sensor Flyer (PDF)
  • Montana Molecular: Detecting Cell Stress in Cor.4U Cardiomyocytes (PDF)