Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Next Generation Fluorescent Probes: GlycoFluor™ Series

GlycoYELLOW™-ßGal: Visualization of ß-Galactosidase and SA-ß-Gal

GlycoYellow
GlycoYellow: Live-Cell Imaging

Live-cell imaging of 293/LacZ (ß-galactosidase expressing cell line) and HEK293 cells by using GlycoYELLOW™–ßGal. Comparing these cell lines, strong fluorescence derived from GlycoYELLOW™–ßGal was observed inside 293/LacZ cells (mean fluorescence intensity increases 5.6 times).

GlycoYellow: Fluorescence Spectra

Fluorescence spectra of GlycoYELLOW™–ßGal before and after hydrolysis by ß-galactosidase.

GlycoYellow: Time-Lapse Observation

Time-lapse observation of 293/LacZ cells (ß-galactosidase expressing cells) treated with GlycoYELLOW™–ßGal. GlycoYELLOW™–ßGal has excellent retention ability inside cells.

GlycoYellow: SA-ß-Gal Staining

Live-Cell Imaging of Senescence-Associated Beta-Galactosidase (SA-ß-Gal).
Live-cell imaging of radiation-induced senescent cells using GlycoYELLOW™-ßGal: yellow signal indicates ß-galactosidase (ß-Gal) activity in living cells.

Features

  • For live-cell imaging
  • High sensitivity
  • High retention in cells
  • λex: 525 nm*; λem: 543 nm
    (*Excitation by a 488 nm laser is also possible.)

Description

  • GlycoYELLOW™–ßGal is a specific fluorescent probe for detection of ß-galactosidase. It can be used for fluorescent imaging and selection of cells and tissues transfected with lacZ. Furthermore, it can be used for detection of Senescence-associated beta-galactosidase (SA-ß-gal) in senescent cells.
  • Because GlycoYELLOW™–ßGal is almost non-fluorescent in the absence of ß-galactosidase, it has a high S/N ratio.

Application

  • • Gene analysis of lacZ gene
  • • Monitoring transfection efficiency
  • • Promoter or enhancer studies
  • • For live-cell imaging of Senescence-associated beta-galactosidase (SA-ß-gal) in senescent cells

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
GC601 GlycoYELLOW™-βGal 50μg×10 727,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe.

Related Products

  • More details regarding the following products can be found on the corresponding Goryo websites: GlycoFluor™ Series
Order# Description Amount Price Data Sheet
GC602 PDF
GC603 GlycoYELLOW™-βGlcU 50μg×10 727,00 PDF
GC1001 EsterYELLOW™-Ac 50μg×10 627,00 PDF
GC611 GlycoGREEN™βGal 30 nmol 527,00 PDF
SK4001-01 TokyoGreen®-βGal 1mg 727,00 PDF
SK4002-01 TokyoGreen®-βGlu 1mg 727,00 PDF
SK4003-01 TokyoGreen®-βGlcU (Na) 1mg 727,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe.

Download

  • Goryo: GlycoYELLOW™ Flyer (PDF)
  • Goryo: GlycoGREEN™ Flyer (PDF)