Innovative Tools for Molecular and Cell Biology

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Miscellaneous Metal Ion Indicators

Miscellaneous Metal Ion Indicators

Description

Several fluorescent indicators can be used to selectively determine polyvalent cation concentrations inside cells or to follow metal ion transport through ion channels. Other indicators are primarily useful for measurements in solutions or in extracts of environmental samples. In most cases, the high affinity of the indicators for metal ions allows interference from other compounds to be minimized by diluting the sample with deionized water. As with Ca2+ and Mg2+ detection, spectroscopic responses to metal ion binding and dissociation constant (Kd) values are dependent on many factors, including pH, temperature, viscosity, protein binding and the presence of other ions. These responses may vary significantly in complex environments such as seawater and the cytosol. Calcein is a relatively low-affinity Ca2+ chelator. The dissociation constants for both the Ca2+ and Mg2+ complexes of calcein at physiological pH are about 10-3 to 10-4 M. As a derivative of iminodiacetic acid, this dye exhibits an ion affinity that increases considerably at higher pH, and thus it is not particularly useful for measuring Ca2+ or Mg2+ in cells. Instead, it is primarily useful for detecting mineralized Ca2+, particularly in the context of bone and bacterial spores. Calcein is also widely used for fluorescence quenching–based detection of Ni2+, Co2+, Cu2+ and Fe3+.

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Miscellaneous Metal Ion Indicators

Order# Description Amount Price Data Sheet
89205AS Calcein blue, AM 1 mg 75,00 PDF
89201AS Calcein, AM 1 mg 156,00 PDF
C1265-1mgAG Calcein-AM 1 mg 226,00 PDF
MFP-C099 Calcein AM (1 mM solution in anhydrous DMSO) 1ml 205,00 PDF
89202AS Calcein, AM *UltraPure Grade* 1 mg 180,00 PDF
MFP-C430 Calcein AM ultrapure grade 1 mg 198,00 PDF
89204AS Calcein; AM *UltraPure Grade* *Small Package* 50 µg x 20 311,00 PDF
89203AS Calcein, AM *UltraPure Grade*, 5 mM solution in anhydrous DMSO 200 µL 203,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

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  • Fluorescent Dyes Brochure (PDF)