Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

HiLyte™ Your Protein With Brilliance

Protein A-HiLyte™ Fluor Conjugates

Protein A-HiLyte™ Fluor 488 conjugate

3T3 cells stained with Rabbit-anti-tubulin IgG (0.5 µg/ml) followed by Protein A-HiLyte™ Fluor 488 conjugate at 0.5 µg/ml.

Protein A-HiLyte™ Fluor 555 conjugate

3T3 cells stained with Rabbit-anti-tubulin IgG (0.5 µg/ml) followed by Protein A-HiLyte™ Fluor 555 conjugate at 0.5 µg/ml.

Protein A-HiLyte™ Fluor 647 conjugate

3T3 cells stained with Rabbit-anti-tubulin IgG (0.5 µg/ml) followed by Protein A-HiLyte™ Fluor 647 conjugate at 0.5 µg/ml.

Western Blot Results

Western Blot Results
3T3 Cell Lysate at 10 µL/lane was resolved on SDS-PAGE and transferred to nitrocellulose membrane. Membrane was probed with rabbit anti-Actin antibodies at 1 µglmL. Anti-Actin antibodies were detected with either Protein A-HiLyte™ Fluor 680 (left; Order# 72238AS) or Protein A-HiLyte™ Fluor 750 (right; Order# 72239AS) reagent at 0.2 µg/mL.

Features

  • Universal: only one reagent to detect multiple targets.
  • Large panel of emission wavelengths: the emission fluorescence of the HiLyte™ Fluor spans the full visible and near infrared spectra.
  • Super bright signal: HiLyte™ Fluor exhibit more intense fluorescence compared to classical dyes (TAMRA, FITC, ROX, etc.).

Description

  • Protein A-HiLyte™ Fluor conjugates are excellent alternatives to dye-labeled secondary antibodies. These highly efficient conjugates constitute a series of universal detection reagents, which combine the binding capacity of Protein A with the high fluorescent intensity of HiLyte™ Fluor dyes.
  • Protein A-HiLyte™ Fluor conjugates provide a super bright signal. They can be used to detect primary antibodies in every protein detection application, e.g., Western blot (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF).

Protein A

  • Protein A is a non-glycosylated cell-wall protein from Staphylococcus aureus containing five IgG binding domains (A, B, C, D, and E). These domains have a strong affinity for the Fc part of immunoglobulins from different species.
  • Highly purified recombinant Protein A was expressed in a recombinant bacterial expression system. It consists of only the IgG binding domains, with a final purity level of >99%. The apparent molecular mass is approximately 30,000 Da compared to 42,000 Da for the native Protein A found in Staphylococcus aureus. Each domain is approximately 60 amino acids long with no cysteine present.

Recombinant Protein A Affinity for Ig From Various Species

Immunoglobulin Species Protein A Binding
Human IgG1, IgG2, IgG4 Strong
Human IgM, IgA Weak
Mouse IgG1 Weak
Mouse IgG2A, IgG2B, IgG3 Strong
Mouse IgM None
Rat IgG1 Weak
Rat IgG2C Strong
Goat IgG1 Weak
Goat IgG2 Strong
Bovine IgG2 Strong
Guinea Pig IgG Strong
Rabbit IgG Strong

HiLyte™ Fluor Dyes

  • HiLyte™ Fluor Dyes are AnaSpec, EGT Group's proprietary Fluorescent Dyes. They are pH insensitive, highly photostable, and brighter than the classical fluorescent dyes. They span the full visible spectrum and can be used for multiple applications.

Related Products

  • Unlabeled recombinant Protein A and biotin-conjugated Protein A are also available. Unlabeled Protein A can be used for immunoprecipitation, antibodies purification, and assay development.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.

Protein A Labeled with HiLyte™ Fluor Dyes

Order# Description Amount Price Data Sheet
72235AS Protein A-HiLyte™ Fluor 488 Conjugate 1 mg 151,00 PDF
72236AS Protein A-HiLyte™ Fluor 555 Conjugate 1 mg 151,00 PDF
72237AS Protein A-HiLyte™ Fluor 647 Conjugate 1 mg 151,00 PDF
72239AS Protein A-HiLyte™ Fluor 750 Conjugate 1 mg 151,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Related product: Protein A - Biotin Conjugate

Order# Description Amount Price Data Sheet
72234AS Protein A-Biotin Conjugate 5 mg 176,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Related product: Unconjugated Protein A

Order# Description Amount Price Data Sheet
72233-5AS Protein A, recombinant 5 mg 88,00 PDF
72233-25AS Protein A, recombinant 25 mg 176,00 PDF
72233-100AS Protein A, recombinant 100 mg 411,00 PDF
72233-1000AS Protein A, recombinant 1000 mg 1371,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Download

  • AnaSpec Protein A - HiLyte™ Fluor Conjugates Flyer (PDF)

Literature

  • Björk, I. Eur J Biochem 29 (1972): 579-584.
  • Sjödahl, J. Eur J Biochem 78 (1977): 471-490.
  • Schröder, A. K. et al. Immunol 57 (1985): 305-309.
  • Rosenberg, I.M. Protein Analysis and Purification, Birkhäuser, 2005: 41-47.