For Specific Detection of Cancer Cells
GORYO CHEMICAL, INC.
Reaction Mechanism of ProteoGREEN™-gGlu and GGT
Fluorescence Spectra of ProteoGREEN™-gGlu after Treatment with GGT
ProteoGREEN™-gGlu was treated with GGT for 30 min at 37 °C. After activation the fluorescence intensity markedly increased at 525 nm (S/N>350).
Live Cell Imaging of Cancer and HUVEC Cells
Each cell type was treated with ProteoGREEN™-gGlu (2 µM) for 1 h. The reaction was completely inhibited by treatment with a GGT-selective inhibitor.
In Vivo Imaging
- Specific for cancer cells
- High S/N ratio
- GGT-activatable fluorescent probe
- ProteoGREEN™-gGlu is almost non-fluorescent in the absence of GGT
- ProteoGREEN™-gGlu is a cancer-selective fluorogenic imaging probe. This probe is rapidly activated by g-glutamyltranspeptidase (GGT), which is known to be a tumor-associated enzyme. This results in a highly fluorescent compound. The membrane-permeable ProteoGREEN™-gGlu is applicable to live cell or in vivo imaging of cancer cells.
- ProteoGREEN™-gGlu is almost non-fluorescent in the absence of GGT. Activation by GGT results in a fluorescence turn-on with high S/N ratio.
- Urano, Y. et al.: Rapid cancer detection by topically spraying a γ-glutamyltranspeptidase-activated fluorescent probe.
Sci. Transl. Med. 2011, 129, 1-10. Pubmed
- Urano Y. et al.: Novel live imaging techniques of cellular functions and in vivo tumors based on precise design of small molecule-based 'activatable' fluorescence probes.
Curr Opin Chem Biol., 2012, 16, 602-608. Pubmed
- Mitsunaga, M. et al.: Fluorescence endoscopic detection of murine colitis-associated colon cancer by topically applied enzymatically rapid-activatable probe.
Gut., 2013, 62, 1179-1186. Pubmed
- Sakabe, M. et al.: Rational design of highly sensitive fluorescence probes for protease and glycosidase based on precisely controlled spirocyclization.
J. Am. Chem. Soc., 2013, 135, 409-414 Pubmed
- ProteoGREEN™-gGlu is defined as gGlu-HMRG in the paper.