Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Fluorescent Dyes

Amine-Containing Dyes

Mechanism of protein labeling with amine-containing dyes

Mechanism of protein labeling with amine-containing dyes

Description

  • Amine-containing dyes are widely used to modify water-soluble biopolymers (such as proteins) using water-soluble carbodiimides (such as EDC) to convert the carboxyl groups of the biopolymers into amide groups. Either NHS or NHSS may be used to improve the coupling efficiency of EDC-mediated protein–carboxylic acid conjugations. A large excess of the amine-containing dyes is usually used for EDC-mediated bioconjugations in concentrated protein solutions at low pH to reduce intra- and inter-protein coupling to lysine residues, a common side reaction. These dyes can also be used for modifications of carbohydrates, glycoproteins, and nucleic acids that are first periodate-oxidized to introduce aldehydes and ketones into the biopolymers for subsequent reductive amination. The combination of periodate oxidation with reductive amination provides an effective way for site-selective modifications of biopolymers.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.

Order# Description Amount Price Data Sheet
MFP-D660-02 MFP™-DYQ-660-Amino function 1 mg 216,00 PDF
MFP-D350-02 MFP™-DY-350-aminomodified 1 mg 216,00 PDF
MFP-D405-02 MFP™-DY-405-aminomodified 1 mg 216,00 PDF
MFP-D415-02 MFP™-DY-415-aminomodified 1 mg 216,00 PDF
MFP-D480XL-02 MFP™-DY-480XL-Amino function 1 mg 216,00 PDF
MFP-D481XL-02 MFP™-DY-481XL-Amino function 1 mg 216,00 PDF
MFP-D485XL-02 MFP™-DY-485XL-Amino function 1 mg 216,00 PDF
81162AS HiLyte™ Fluor 488 amine, TFA Salt 1 mg 195,00 PDF
MFP-D490-02 MFP™-DY-490-aminomodified 1 mg 216,00 PDF
MFP-D495-X5-02 MFP™-DY-495-X5-aminomodified 5 mg 752,00 PDF
MFP-D500XL-02 MFP™-DY-500XL-Amino function 1 mg 216,00 PDF
MFP-D505-X5-02 MFP™-DY-505-X5-Amino function 1 mg 360,00 PDF
MFP-D510XL-02 MFP™-DY-510XL-amino function 1 mg 216,00 PDF
MFP-D520XL-02 MFP™-DY-520XL-Amino function 1 mg 216,00 PDF
MFP-D521XL-02 MFP™-DY-521XL-Amino function 1 mg 216,00 PDF
MFP-D547P1-02 MFP™-DY-547P1-aminomodified 1 mg 216,00 PDF
MFP-D549P1-02 MFP™-DY-549P1-aminomodified 1 mg 216,00 PDF
MFP-D550-02 MFP™-DY-550-Amino function 1 mg 216,00 PDF
MFP-D555-02 MFP™-DY-555-Amino function 1 mg 216,00 PDF
81252AS HiLyte™ Fluor 555 amine 1 mg 239,00 PDF
MFP-D556-02 MFP™-DY-556-Amino function 1 mg 216,00 PDF
MFP-D590-02 MFP™-DY-590-Amino function 1 mg 216,00 PDF
MFP-D594-02 MFP™-DY-594-Amino function 1 mg 216,00 PDF
MFP-D605-02 MFP™-DY-605-Amino function 1 mg 216,00 PDF
MFP-D610-02 MFP™-DY-610-Amino function 1 mg 216,00 PDF
MFP-D615-02 MFP™-DY-615-Amino function 1 mg 216,00 PDF
MFP-D630-02 MFP™-DY-630-Amino function 1 mg 216,00 PDF
MFP-D631-02 MFP™-DY-631-Amino function 1 mg 216,00 PDF
MFP-D632-02 MFP™-DY-632-Amino function 1 mg 216,00 PDF
MFP-D633-02 MFP™-DY-633-Amino function 1 mg 216,00 PDF
MFP-D634-02 MFP™-DY-634-Amino function 1 mg 216,00 PDF
MFP-D635-02 MFP™-DY-635-Amino function 1 mg 216,00 PDF
MFP-D636-02 MFP™-DY-636-Amino function 1 mg 216,00 PDF
81257AS HiLyte™ Fluor 647 amine 1 mg 239,00 PDF
MFP-D649P1-02 MFP™-DY-649P1-Amino function 1 mg 216,00 PDF
MFP-D650-02 MFP™-DY-650-Amino function 1 mg 216,00 PDF
MFP-D651-02 MFP™-DY-651-Amino function 1 mg 216,00 PDF
MFP-D654-02 MFP™-DY-654-Amino function 1 mg 216,00 PDF
MFP-D675-02 MFP™-DY-675-Amino function 1 mg 216,00 PDF
MFP-D676-02 MFP™-DY-676-Amino function 1 mg 216,00 PDF
MFP-D678-02 MFP™-DY-678-Amino function 1 mg 216,00 PDF
MFP-D679P1-02 MFP™-DY-679P1-Amino function 1 mg 216,00 PDF
MFP-D680-02 MFP™-DY-680-Amino function 1 mg 216,00 PDF
MFP-D681-02 MFP™-DY-681-Amino function 1 mg 216,00 PDF
MFP-D682-02 MFP™-DY-682-Amino function 1 mg 216,00 PDF
MFP-D700-02 MFP™-DY-700-Amino function 1 mg 216,00 PDF
MFP-D701-02 MFP™-DY-701-Amino function 1 mg 216,00 PDF
MFP-D703-02 MFP™-DY-703-Amino function 1 mg 216,00 PDF
MFP-D730-02 MFP™-DY-730-Amino function 1 mg 216,00 PDF
MFP-D731-02 MFP™-DY-731-Amino function 1 mg 216,00 PDF
MFP-D732-02 MFP™-DY-732-Amino function 1 mg 216,00 PDF
MFP-D734-02 MFP™-DY-734-Amino function 1 mg 216,00 PDF
MFP-D750-02 MFP™-DY-750-Amino function 1 mg 216,00 PDF
81267AS HiLyte™ Fluor 750 amine 1 mg 251,00 PDF
MFP-D751-02 MFP™-DY-751-Amino function 1 mg 216,00 PDF
MFP-D752-02 MFP™-DY-752-Amino function 1 mg 216,00 PDF
MFP-D754-02 MFP™-DY-754-Amino function 1 mg 216,00 PDF
MFP-D776-02 MFP™-DY-776-Amino function 1 mg 216,00 PDF
MFP-D777-02 MFP™-DY-777-Amino function 1 mg 216,00 PDF
MFP-D780-02 MFP™-DY-780-Amino function 1 mg 216,00 PDF
MFP-D781-02 MFP™-DY-781-Amino function 1 mg 216,00 PDF
MFP-D782-02 MFP™-DY-782-Amino function 1 mg 216,00 PDF
MFP-D800-02 MFP™-DY-800-Amino function 1 mg 216,00 PDF
MFP-D831-02 MFP™-DY-831-Amino function 1 mg 216,00 PDF
  • All prices are in EURO excl. VAT and shipping. AnaSpec dyes are only available in selected countries.
    For further pricing and order information please ask your local distributor.

Download

  • Fluorescent Probes for Protein and Nucleic Acid Labeling Handbook (PDF)
  • Fluorescent Cell Organelle Stains Brochure (PDF)
  • Fluorophore Selection Guide Poster (7MB) (PDF)