Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Fluorescent Dyes

Amine-Reactive Dyes

Mechanism of labeling with succinimidyl ester dyes

Mechanism of labeling with succinimidyl ester dyes

TFP ester

TFP ester

Mechanism of labeling with sulfonyl chloride dyes

Mechanism of labeling with sulfonyl chloride dyes

Mechanism of labeling with isothiocyanate dyes

Mechanism of labeling with isothiocyanate dyes

Description

  • A number of fluorescent amine-reactive dyes have been developed to label various biomolecules, and the resultant conjugates are widely used in biological applications. There are four major classes of reactive fluorescent reagents used to label amines: succinimidyl esters (SE), isothiocyanates (ITC), sulfonyl chlorides (SC), and electron-deficient aryl halides. We offer all the popular amine-reactive fluorescent dyes for peptide/protein labelings, nucleotide modifications, and microarray applications.

Carboxylic acids and their succinimidyl esters

  • Succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds that are formed are essentially identical to, and as stable as peptide bonds. These reagents are generally stable if they are properly stored, and show good reactivity and selectivity with aliphatic amines. They have very low reactivity with aromatic amines, alcohols, phenols (including tyrosine) and histidine.

TFP esters

  • Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions.

Sulfonyl chlorides

  • Sulfonyl chlorides, including Dansyl chloride, Lissamine™ rhodamine B sulfonyl chloride, and sulforhodamine 101 sulfonyl chloride (also known as Texas Red®) are highly reactive. These reagents are unstable in water, especially at the higher pH required for reaction with aliphatic amines. Protein modifications by sulfonyl chlorides need to be carefully carried out preferably at low temperature. Sulfonyl chlorides can also react with phenols (including tyrosine), aliphatic alcohols (including polysaccharides), thiols (such as cysteine), and imidazoles (such as histidine), but these reactions are not common in proteins or in aqueous solution. Sulfonyl chlorides are unstable in dimethylsulfoxide (DMSO) and should never be used in this solvent.

Isothiocyanates

  • Isothiocyanates form thioureas upon reaction with amines. Studies indicated that some thiourea products (in particular, the conjugates from a-amino acids/peptides/proteins) are much less stable than the conjugates that are prepared from the corresponding succinimidyl esters. It has been reported that antibody conjugates prepared from fluorescein isothiocyanates deteriorate over time. Thus, only a few popular isothiocyanates are offered: 5- and 6-fluorescein isothiocyanates (FITC) that are still widely used for preparing fluorescent antibody conjugates primarily due to their low costs. However, we strongly recommend using 5-FAM, SE, when necessary, for your conjugations whenever possible.
  • For more information including the Ex/Em (nm) of each dye, please refer to our Fluorescent Probes for Protein and Nucleic Acid Labeling Handbook (PDF).
  • Protocol for Labeling With Amine-Reactive Probes (PDF)

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
81228AS DNP-X acid, SE 25 mg 69,00 PDF
81801AS DABCYL acid, SE 100 mg 91,00 PDF
81804AS DABCYL Plus™ acid, SE 25 mg 126,00 PDF
81826-5AS QXL® 490 acid, SE 5 mg 201,00 PDF
81836-5AS QXL®570 acid, SE 5 mg 389,00 PDF
81816-5AS QXL® 610 acid, SE 5 mg 201,00 PDF
MFP-D660-01-1 MFP™-DYQ-660-NHS-Ester 1 mg 168,00 PDF
81841AS QXL® 670 acid, SE 5 mg 389,00 PDF
81214AS Dansyl-X, SE 25 mg 86,00 PDF
MFP-D350-01-1 MFP™-DY-350-NHS-Ester 1 mg 168,00 PDF
MFP-D350-06-1 MFP™-DY-350-TFP-Ester 1 mg 168,00 PDF
81208AS AMCA-X, SE 10 mg 126,00 PDF
MFP-D405-01-1 MFP™-DY-405-NHS-Ester 1 mg 168,00 PDF
MFP-D405-06-1 MFP™-DY-405-TFP-Ester 1 mg 168,00 PDF
89317-1AS HiLyte™ Fluor 405 acid, SE 1 mg 149,00 PDF
MFP-D415-01-1 MFP™-DY-415-NHS-Ester 1 mg 168,00 PDF
81211AS DEAC, SE [7-Diethylaminocoumarin-3-carboxylic acid, succinimidyl ester] 25 mg 97,00 PDF
81213AS NBD-X, SE 25 mg 140,00 PDF
MFP-D480XL-01-1 MFP™-DY-480XL-NHS-Ester 1 mg 168,00 PDF
MFP-D481XL-01-1 MFP™-DY-481XL-NHS-Ester 1 mg 168,00 PDF
MFP-D485XL-01-1 MFP™-DY-485XL-NHS-Ester 1 mg 168,00 PDF
MFP-A2000 MFP488-NHS (Protein Labeling) 1 mg 264,00 PDF
MFP-D490-01-1 MFP™-DY-490-NHS-Ester 1 mg 168,00 PDF
MFP-D490-06-1 MFP™-DY-490-TFP-Ester 1 mg 168,00 PDF
89000AS 5(6)-CFDA, SE 25 mg 126,00 PDF
81007AS 5-FAM, SE 10 mg 103,00 PDF
81007-100AS 5-FAM, SE 100 mg 383,00 PDF
MFP-D495-X5-01-1 MFP™-DY-495-X5-NHS-Ester 5 mg 190,00 PDF
81006AS 5(6)-FAM, SE 25 mg 69,00 PDF
81006-100AS 5(6)-FAM, SE 100 mg 126,00 PDF
81006-1000AS 5(6)-FAM, SE 1 g 909,00 PDF
20151AS 5-FITC 1 g 132,00 PDF
81009AS 5-FAM-X, SE 5 mg 103,00 PDF
81008AS 6-FAM, SE 10 mg 126,00 PDF
81008-100AS 6-FAM, SE 100 mg 383,00 PDF
81161-1AS HiLyte™ Fluor 488 acid, SE 1 mg 149,00 PDF
81161AS HiLyte™ Fluor 488 acid, SE 5 mg 429,00 PDF
MFP-D505-X5-01-1 MFP™-DY-505-X5-NHS-ester 5 mg 438,00 PDF
MFP-D500XL-01-1 MFP™-DY-500XL-NHS-Ester 1 mg 168,00 PDF
MFP-D510XL-01-1 MFP™-DY-510XL-NHS-Ester 1 mg 168,00 PDF
MFP-D520XL-01-1 MFP™-DY-520XL-NHS-Ester 1 mg 168,00 PDF
81022AS 6-TET, SE 5 mg 188,00 PDF
81104AS 5(6)-CR6G, SE 10 mg 165,00 PDF
MFP-D521XL-01-1 MFP™-DY-521XL-NHS-Ester 1 mg 168,00 PDF
81106AS 6-CR6G, SE 5 mg 188,00 PDF
81105AS 5-CR6G, SE 5 mg 188,00 PDF
81020AS 6-HEX, SE 5 mg 213,00 PDF
MFP-D530-01-1 MFP™-DY-530-NHS-Ester 1 mg 168,00 PDF
81150AS 5(6)-TRITC 10 mg 121,00 PDF
81127AS 5(6)-TAMRA-X, SE 5 mg 126,00 PDF
81124AS 5(6)-TAMRA, SE 25 mg 132,00 PDF
81124-01AS 5(6)-TAMRA, SE 100 mg 383,00 PDF
MFP-D547P1-01-1 MFP™-DY-547P1-NHS-Ester 1 mg 168,00 PDF
MFP-D549P1-01-1 MFP™-DY-549P1-NHS-Ester 1 mg 168,00 PDF
MFP-D549P1-06-1 MFP™-DY-549P1-TFP-Ester 1 mg 168,00 PDF
MFP-D550-01-1 MFP™-DY-550-NHS-Ester 1 mg 168,00 PDF
MFP-D554-01-1 MFP™-DY-554-NHS-Ester 1 mg 168,00 PDF
MFP-D555-01-1 MFP™-DY-555-NHS-Ester 1 mg 168,00 PDF
MFP-A2009 MFP-555 NHS-Ester 1 mg 290,00 PDF
81126AS 6-TAMRA, SE 5 mg 132,00 PDF
81125AS 5-TAMRA, SE 5 mg 132,00 PDF
81125-01AS 5-TAMRA, SE 100 mg 1040,00 PDF
81251AS HiLyte™ Fluor 555 acid, SE 1 mg 246,00 PDF
MFP-D556-01-1 MFP™-DY-556-NHS-Ester 1 mg 168,00 PDF
MFP-D560-01-1 MFP™-DY-560-NHS-Ester 1 mg 168,00 PDF
81114AS 5-ROX, SE 5 mg 188,00 PDF
81115AS 6-ROX, SE 5 mg 188,00 PDF
81113AS 5(6)-ROX, SE 25 mg 188,00 PDF
MFP-A2004 MFP-590 NHS-Ester 1 mg 264,00 PDF
MFP-D590-01-1 MFP™-DY-590-NHS-Ester 1 mg 168,00 PDF
MFP-D591-01-1 MFP™-DY-591-NHS-Ester 1 mg 168,00 PDF
81130AS Sulforhodamine 101 sulfonyl chloride 10 mg 103,00 PDF
81272-1AS HiLyte™ Fluor 594 acid, SE 1 mg 149,00 PDF
81272-5AS HiLyte™ Fluor 594 acid, SE 5 mg 429,00 PDF
MFP-D594-01-1 MFP™-DY-594-NHS-Ester 1 mg 168,00 PDF
MFP-D594-06-1 MFP™-DY-594-TFP-Ester 1 mg 168,00 PDF
MFP-D605-01-1 MFP™-DY-605-NHS-Ester 1 mg 168,00 PDF
MFP-D610-01-1 MFP™-DY-610-NHS-Ester 1 mg 168,00 PDF
MFP-D615-01-1 MFP™-DY-615-NHS-Ester 1 mg 168,00 PDF
MFP-D630-01-1 MFP™-DY-630-NHS-Ester 1 mg 168,00 PDF
MFP-D631-01-1 MFP™-DY-631-NHS-Ester 1 mg 168,00 PDF
MFP-A2005 MFP-631-NHS-Ester 1 mg 290,00 PDF
MFP-D632-01-1 MFP™-DY-632-NHS-Ester 1 mg 168,00 PDF
MFP-D633-01-1 MFP™-DY-633-NHS-Ester 1 mg 168,00 PDF
MFP-D634-01-1 MFP™-DY-634-NHS-Ester 1 mg 168,00 PDF
MFP-D634-06-1 MFP™-DY-634-TFP-Ester 1 mg 168,00 PDF
MFP-D635-01-1 MFP™-DY-635-NHS-Ester 1 mg 168,00 PDF
MFP-D636-01-1 MFP™-DY-636-NHS-Ester 1 mg 168,00 PDF
81256AS HiLyte™ Fluor 647 acid, SE 1 mg 246,00 PDF
MFP-D647P1-01-1 MFP™-DY-647P1-NHS-Ester 1 mg 168,00 PDF
MFP-D648P1-01-1 MFP™-DY-648P1-NHS-Ester 1 mg 168,00 PDF
MFP-D649P1-01-1 MFP™-DY-649P1-NHS-Ester 1 mg 168,00 PDF
MFP-D649P1-06-1 MFP™-DY-649P1-TFP-Ester 1 mg 168,00 PDF
MFP-D650-01-1 MFP™-DY-650-NHS-Ester 1 mg 168,00 PDF
MFP-D654-01-1 MFP™-DY-654-NHS-Ester 1 mg 168,00 PDF
MFP-D651-01-1 MFP™-DY-651-NHS-Ester 1 mg 168,00 PDF
MFP-D652-01-1 MFP™-DY-652-NHS-Ester 1 mg 168,00 PDF
MFP-D660-01-1 MFP™-DYQ-660-NHS-Ester 1 mg 168,00 PDF
MFP-D675-01-1 MFP™-DY-675-NHS-Ester 1 mg 168,00 PDF
MFP-D676-01-1 MFP™-DY-676-NHS-Ester 1 mg 168,00 PDF
MFP-D677-01-1 MFP™-DY-677-NHS-Ester 1 mg 168,00 PDF
MFP-D678-01-1 MFP™-DY-678-NHS-Ester 1 mg 168,00 PDF
MFP-D679P1-01-1 MFP™-DY-679P1-NHS-Ester 1 mg 168,00 PDF
MFP-D680-01-1 MFP™-DY-680-NHS-Ester 1 mg 168,00 PDF
MFP-D681-01-1 MFP™-DY-681-NHS-Ester 1 mg 168,00 PDF
MFP-D682-01-1 MFP™-DY-682-NHS-Ester 1 mg 168,00 PDF
MFP-D682-06-1 MFP™-DY-682-TFP-Ester 1 mg 168,00 PDF
MFP-D700-01-1 MFP™-DY-700-NHS-Ester 1 mg 168,00 PDF
MFP-D701-01-1 MFP™-DY-701-NHS-Ester 1 mg 168,00 PDF
MFP-D703-01-1 MFP™-DY-703-NHS-Ester 1 mg 168,00 PDF
MFP-D704-01-1 MFP™-DY-704-NHS-Ester 1 mg 168,00 PDF
MFP-D730-01-1 MFP™-DY-730-NHS-Ester 1 mg 168,00 PDF
MFP-D731-01-1 MFP™-DY-731-NHS-Ester 1 mg 168,00 PDF
MFP-D732-01-1 MFP™-DY-732-NHS-Ester 1 mg 168,00 PDF
MFP-D734-01-1 MFP™-DY-734-NHS-Ester 1 mg 168,00 PDF
MFP-D749P1-01-1 MFP™-DY-749P1-NHS-Ester 1 mg 168,00 PDF
MFP-D750-01-1 MFP™-DY-750-NHS-Ester 1 mg 168,00 PDF
81266AS HiLyte™ Fluor 750 acid, SE 1 mg 246,00 PDF
MFP-D751-01-1 MFP™-DY-751-NHS-Ester 1 mg 168,00 PDF
MFP-D752-01-1 MFP™-DY-752-NHS-Ester 1 mg 168,00 PDF
MFP-D754-01-1 MFP™-DY-754-NHS-Ester 1 mg 168,00 PDF
MFP-D776-01-1 MFP™-DY-776-NHS-Ester 1 mg 168,00 PDF
MFP-D776-06-1 MFP™-DY-776-TFP-Ester 1 mg 168,00 PDF
MFP-D777-01-1 MFP™-DY-777-NHS-Ester 1 mg 168,00 PDF
MFP-D778-01-1 MFP™-DY-778-NHS-Ester 1 mg 168,00 PDF
MFP-D780-01-1 MFP™-DY-780-NHS-Ester 1 mg 168,00 PDF
MFP-D781-01-1 MFP™-DY-781-NHS-Ester 1 mg 168,00 PDF
MFP-D782-01-1 MFP™-DY-782-NHS-Ester 1 mg 168,00 PDF
MFP-D800-01-1 MFP™-DY-800-NHS-Ester 1 mg 168,00 PDF
MFP-D800-06-1 MFP™-DY-800-TFP-Ester 1 mg 168,00 PDF
MFP-D831-01-1 MFP™-DY-831-NHS-Ester 1 mg 168,00 PDF
  • All prices are in EURO excl. VAT and shipping. AnaSpec dyes are only available in selected countries.
    For further pricing and order information please ask your local distributor.

Download

  • Fluorescent Probes for Protein and Nucleic Acid Labeling Handbook (PDF)
  • Protocol for Labeling With Amine-Reactive Probes (PDF)
  • Fluorescent Cell Organelle Stains Brochure (PDF)
  • Fluorophore Selection Guide Poster (7MB) (PDF)
  • Fluorescent Dyes Brochure (PDF)