Fig. 1 Structures of A) a hydrazide, B) a semicarbazide, and C) a carbohydrazide
Fig. 2 Modifying aldehydes and ketones with hydrazine derivatives
- Fluorescent hydrazides are carbonyl-reactive fluorescent labeling dyes. They can be used for labeling glycoproteins such as HRP. Although certain aromatic amines such as 8-aminonaphthalene-1,3,6- trisulfonic acid (ANTS) and 8-aminopyrene-1,3,6-trisulfonic acid (APTS) have been extensively utilized to modify reducing sugars for analysis and sequencing, the most reactive reagents for forming stable conjugates of aldehydes and ketones are usually hydrazine derivatives, including hydrazides, semicarbazides and carbohydrazides (Fig. 1), as well as hydroxylamine derivatives. Hydrazine derivatives react with ketones to yield relatively stable hydrazones (Fig. 2), and with aldehydes to yield hydrazones that are somewhat less stable, though they may be formed faster. In addition, hydrazides are low molecular weight, cell membrane-impermeant, aldehyde-fixable molecules that can be used as a cell tracer, e.g., by loading into cells by microinjection, infusion from patch pipette, or uptake induced by pinocytic cell-loading reagents.
- For information regarding the Ex/Em (nm) of each dye, please refer to our Fluorescent Probes for Protein and Nucleic Acid Labeling Handbook.
- For shipping and storage information please click on Order#.
|89319AS||HiLyte™ Fluor 405 hydrazide||1 mg||178,00|
|81163AS||HiLyte™ Fluor 488 hydrazide||1 mg||178,00|
|81274AS||HiLyte™ Fluor 594 hydrazide - TFA Salt||1 mg||178,00|
|81268AS||HiLyte™ Fluor 750 hydrazide||1 mg||229,00|
- All prices are in EURO excl. VAT and shipping. AnaSpec dyes are only available in selected countries.
For further pricing and order information please ask your local distributor.