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Fluorescent Dyes

Carbonyl-Reactive Hydrazides

Structures of a hydrazide, a semicarbazide, and a carbohydrazide

Fig. 1 Structures of A) a hydrazide, B) a semicarbazide, and C) a carbohydrazide

Modifying aldehydes and ketones with hydrazine derivatives

Fig. 2 Modifying aldehydes and ketones with hydrazine derivatives

Description

  • Fluorescent hydrazides are carbonyl-reactive fluorescent labeling dyes. They can be used for labeling glycoproteins such as HRP. Although certain aromatic amines such as 8-aminonaphthalene-1,3,6- trisulfonic acid (ANTS) and 8-aminopyrene-1,3,6-trisulfonic acid (APTS) have been extensively utilized to modify reducing sugars for analysis and sequencing, the most reactive reagents for forming stable conjugates of aldehydes and ketones are usually hydrazine derivatives, including hydrazides, semicarbazides and carbohydrazides (Fig. 1), as well as hydroxylamine derivatives. Hydrazine derivatives react with ketones to yield relatively stable hydrazones (Fig. 2), and with aldehydes to yield hydrazones that are somewhat less stable, though they may be formed faster. In addition, hydrazides are low molecular weight, cell membrane-impermeant, aldehyde-fixable molecules that can be used as a cell tracer, e.g., by loading into cells by microinjection, infusion from patch pipette, or uptake induced by pinocytic cell-loading reagents.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
89319AS HiLyte™ Fluor 405 hydrazide 1 mg 195,00 PDF
MFP-A1436 MFP488-Hydrazide 1 mg 205,00 PDF
81163AS HiLyte™ Fluor 488 hydrazide 1 mg 195,00 PDF
81274AS HiLyte™ Fluor 594 hydrazide - TFA Salt 1 mg 195,00 PDF
MFP-D594-04 MFP™-DY-594-Hydrazide 1 mg 168,00 PDF
MFP-D634-04 MFP™-DY-634-Hydrazide 1 mg 168,00 PDF
MFP-D681-04 MFP™-DY-681-Hydrazide 1 mg 216,00 PDF
MFP-D682-04 MFP™-DY-682-Hydrazide 1 mg 216,00 PDF
81268AS HiLyte™ Fluor 750 hydrazide 1 mg 251,00 PDF
MFP-D782-04 MFP™-DY-782-Hydrazide 1 mg 216,00 PDF
  • All prices are in EURO excl. VAT and shipping. AnaSpec dyes are only available in selected countries.
    For further pricing and order information please ask your local distributor.

Download

  • Fluorescent Probes for Protein and Nucleic Acid Labeling Handbook (PDF)
  • Fluorescent Cell Organelle Stains Brochure (PDF)
  • Fluorophore Selection Guide Poster (7MB) (PDF)