Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

EosFP Fusion Proteins - Ready-to-Use Constructs

pwt-mitoEosFP (with mitochondrial targeting signal) (Order no: VS-FLP10010)

Features

  • Expression vector for fluorescent tetrameric wt-EosFP with mitochondrial targeting signal
  • Green to red photoconvertible
  • Permanent, bright and fast
  • Stable at 37 °C and below
  • Labeling and tracking of mitochondria
  • Selective marking of selected mitochondria by photoconversion
  • Ready-to-use construct for transfection and subsequent microscopy analyses
  • Applicable to high resolution fluorescence microscopy like PALM

Description

"pwt-mitoEosFP (with mitochondrial targeting signal)" is an expression vector encoding for the fluorescent green-to-red photoconvertible wildtype EosFP protein (wt-EosFP) already attached to a mitochondrial targeting signal. This fusion protein is functionally expressed in a wide range of pro- and eukaryotic cells at a temperature of 37 °C and below.
The construct "pwt-mitoEosFP (with mitochondrial targeting signal)" can be used directly for transfection or microinjection and subsequent analyses by fluorescence microscopy. The mitochondria or parts thereof can be labeled by photoconversion and their movement can be monitored subsequently. The photoconversion feature also enables the use of high resolution fluorescence microscopy like PALM.

td-EosFP Fusions

Features

  • Expression vectors for photoconvertible fluorescent fusion proteins
  • Stable at 37 °C and below
  • Ready-to-use constructs for transfection and subsequent microscopy analyses
  • The pseudomonomeric tandem dimer td-EosFP fused to:
  1. Paxillin (ptd-EosFP-Paxillin) (Order no: VS-FLPC1011)
  2. CD8α (ptd-EosFP-CD8α)(Order no: VS-FLPC1012)
  3. BAP31 (ptd-EosFP-BAP31)(Order no: VS-FLPC1013)

Description

  • "ptd-EosFP-Fusions" are mammalian expression vectors encoding for a fluorescent protein, the green-to-red photoconvertible EosFP tandem dimer (functional monomer), fused to the N-terminus of one of the proteins Paxillin, CD8α and BAP31.
  • Paxillin: a signal transduction adapter protein associated with focal adhesions.
  • CD8α: a cell surface glycoprotein of cytotoxic T cells that kill antigen-bearing target cells.
  • BAP31: an integral membrane protein of the endoplasmic reticulum (ER).

Applications

  • Localization studies, counting and cluster analyses
  • Regional cellular marking of fusion proteins by photoconversion
  • Suitable as control and adjusting sample for any fluorescence microscopy application, including high resolution fluorescence microscopy like PALM
Microscopy images of cells expressing the fusion protein tdEosFP-Paxillin
    Microscopy images of cells expressing the fusion protein tdEosFP-Paxillin, which accumulates in focal adhesions
  • (A, C) PALM
  • (B, D) Sum Wide Field Microscopy.
  • In (C) and (D) zoomed-in views of a focal adhesion complex of the indicated area of (A) and (B) are shown.
  • Scale bars are 5 µm (A) and 0.5 µm (C). The profiles in (C) and (D) are along the indicated arrows. For better comparison, the profile of PALM (C) is also displayed in the sum wide field image (D) in light orange (Carl Zeiss Microscopy GmbH).

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
VS-FLP10010 pwt-mitoEosFP, with mitochondrial targeting signal, lyophilized DNA 10 µg 537,00 PDF
VS-FLPC1011 ptd-EosFP-Paxillin, FLAG-tagged, (FLAG is a registered trademark of Sigma-Aldrich Co), lyophilized DNA 20 µg 548,00 PDF
VS-FLPC1012 ptd-EosFP-CD8, lyophilized DNA 20 µg 548,00 PDF
VS-FLPC1013 ptd-EosFP-BAP31, lyophilized DNA 20 µg 548,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

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Vector maps and Sequences

Vector map of pwt-mitoEosFP (pic)
Vector map of ptd-EosFP-Paxillin (pic)
Vector map of ptd-EosFP-CD8α (pic)
Vector map of ptd-EosFP-BAP31 (pic)

Sequence of pwt-mitoEosFP (txt)
Sequence of ptd-EosFP-Paxillin (txt)
Sequence of ptd-EosFP-CD8α (txt)
Sequence of ptd-EosFP-BAP31 (txt)