Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Label IT® RNAi Delivery Controls

Fluorescently labeled RNAi duplexes ideal for in vitro and in vivo tracking

Visualization of the LabelIT Cy 3 RNAi Delivery Control

Visualization of the Label IT® Cy™3 RNAi Delivery Control in Liver Sections Following Tail Vein Injection.
TransIT®-QR Hydrodynamic Delivery Solution was used to deliver 25 µg of Label IT® Cy™3 RNAi Delivery Control (red) to a mouse using hydrodynamic delivery via the tail vein. Forty-five minutes post-injection the liver was harvested. Sections were processed to stain the nuclei (blue) and to stain the actin (green).

LabelIT RNAi Delivery Controls

The Label IT® RNAi Delivery Controls Allow Quick Assessment of Delivery Efficiency For In Vitro Applications
HeLa cells were transfected in serum-containing media with (A) Label IT® Cy™3 RNAi Delivery Control (red) using theTransIT-siQUEST® Transfection Reagent and (B) Label IT® Fluorescein RNAi Delivery Control (green) using the TransIT-TKO® Transfection Reagent. Twenty-four hours post- transfection, the cells were fixed, then counterstained to locate the nuclei (blue) and the actin (green).

Features

  • Sensitive – Easily visualize transfected cells and assess delivery efficiency using fluorescent microscopy
  • Inert – Not known to target any mammalian genes or cause off-target effects
  • Compatible – Suitable for co-delivery experiments with functional siRNA
  • Ease of Use – Supplied as a ready to use 10 µM stock solution with a 10X RNAi Dilution Buffer

Description

  • The introduction of short RNA duplexes into mammalian cells in culture leads to sequence-specific destruction of target mRNA without triggering an interferon response. These short double stranded (ds) RNAs, referred to as small interfering RNAs (siRNA), can act catalytically at sub-molar ratios to cleave greater than 95% of the target mRNA in the cell and destruction of the mRNA target can ultimately lead to decreased expression of the encoded protein. The RNA interference (RNAi) effect can be long-lasting and may be detectable after many cell divisions. These properties make siRNA extremely effective at inhibiting target gene expression once introduced into the cell.
    The Label IT® RNAi Delivery Control consists of Cy™3- or fluorescein-labeled double-stranded RNA duplexes that have the same length, charge, and configuration as standard siRNA used in RNAi studies. The sequence of the Label IT® RNAi Delivery Control is not homologous to any known mammalian gene and is not known to affect any cellular events. The Label IT® RNAi Delivery Control is designed as a tool to facilitate visualization and optimization of dsRNA oligonucleotide delivery during RNAi experiments, both in vitro and in vivo. It is also suitable for co-delivery with functional target-gene specific siRNA and should not affect the RNAi-mediated inhibition of the target gene.

Fluorophore Spectral Characteristics

Fluorophore Excitation Wevelength or λmax(nm) Emission Wavelength (nm)
Cy™3 550 570
Fluorescein 494 518

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR7900 Label IT® RNAi Delivery Control, Cy®3 10 µg (0.75 nmol) 148,00 PDF
MIR7901 Label IT® RNAi Delivery Control, Cy®3 100 µg (7.5 nmol) 719,00 PDF
MIR7902 Label IT® RNAi Delivery Control, Fluorescein 10 µg (0.75 nmol) 114,00 PDF
MIR7903 Label IT® RNAi Delivery Control, Fluorescein 100 µg (7.5 nmol) 540,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Related Products

Order# Description Amount Price Data Sheet
MIR5210 TransIT®-QR Starter Kit 10 injections 120,00 PDF
MIR2110 TransIT-siQUEST® Transfection Reagent 1.5 ml 442,00 PDF
MIR2150 TransIT-TKO® Transfection Reagent 1.5 ml 442,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.