Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Label IT® Nucleic Acid Labeling Kits

Efficient, Direct, Non-Enzymatic Labeling of DNA and RNA

Non-destructive direct labeling of nucleic acid

Non-destructive direct
labeling of nucleic acid.

Mouse total RNA was labeled with Label IT® Cy™3 (B), electrophoresed and analyzed alongside unlabeled total RNA (A) on an Agilent RNA 6000 Nano chip. RNA Integrity Number (RIN) is approximately 9.0 for both labeled and unlabeled samples.

Schematic overview on LabelIT labeling procedure

The Label IT® nucleic acid labeling kits.
The Label IT® chemical labeling reagents are composed of three regions: the label (fluorophore or hapten) (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids, and the reactive alkylating group (blue) that covalently attaches the Label IT® Reagent to any reactive heteroatom within the nucleic acids. Attachment of the Label IT® Reagents to nucleic acids does not alter the structure of the nucleic acid or affect downstream hybridization performance, and as such, nucleic acids labeled using the Label IT® Reagents can be employed in multiple applications as defined by the researcher.

Features

  • Label any DNA or RNA template – Suitable for a wide range of applications.
  • One-step chemical method – Easily and consistently control the labeling reactions.
  • Adjustable labeling density – Achieve high sensitivity with optimally labeled DNA and RNA.
  • Covalent mechanism – Permanent, non-destructive modification of nucleic acid residues is ideal for many diverse applications; labels do not impact hybridization performance.

Description

  • The Label IT® Technology is a non-enzymatic chemical labeling method that facilitates the direct covalent attachment of either fluorescent or non-fluorescent labels to any nucleic acid using a simple one-step chemical reaction. Mirus Bio optimized the Label IT® Technology for specialized applications including: FISH, microarrays, and nucleic acid visualization both in vitro and in vivo.

Fluorophore Spectral Characteristics

Fluorophore Excitation Wevelength or λmax(nm) Emission Wavelength (nm)
Cy™3 550 570
Cy™5 649 670
CX-Rhodamine 576 597
TM-Rhodamine 546 576
Fluorescein 494 518

Kit Components:

  • Each Kit contains a Label IT® Reagent, Label IT® Reconstitution Solution, 10X Labeling Buffer, and solutions.

  • NOTE: All the Label IT® Reagents use the same technology, but each attach different fluorophores with distinct excitation and emission spectra.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR3900 Label IT® Nucleic Acid Modifying Kit, Amine Sufficient reagent for modifying 100 µg of nucleic acid 642,00 PDF
MIR3925 Label IT® Nucleic Acid Modifying Kit, Amine Sufficient reagent for modifying 25 µg of nucleic acid 258,00 PDF
MIR3400 Label IT® Nucleic Acid Labeling Kit, Biotin labels 100 µg nucleic acid 544,00 PDF
MIR3425 Label IT® Nucleic Acid Labeling Kit, Biotin labels 25 µg nucleic acid 218,00 PDF
MIR3800 Label IT® Nucleic Acid Labeling Kit, DNP labels 100 µg nucleic acid 544,00 PDF
MIR3825 Label IT® Nucleic Acid Labeling Kit, DNP labels 25 µg nucleic acid 218,00 PDF
MIR3300 Label IT® Nucleic Acid Labeling Kit, Digoxin labels 100 µg nucleic acid 544,00 PDF
MIR3325 Label IT® Nucleic Acid Labeling Kit, Digoxin labels 25 µg nucleic acid 218,00 PDF
MIR3200 Label IT® Nucleic Acid Labeling Kit, Fluorescein labels 100 µg nucleic acid 544,00 PDF
MIR3225 Label IT® Nucleic Acid Labeling Kit, Fluorescein labels 25 µg nucleic acid 218,00 PDF
MIR3100 Label IT® Nucleic Acid Labeling Kit, CX-Rhodamine labels 100 µg nucleic acid 544,00 PDF
MIR3125 Label IT® Nucleic Acid Labeling Kit, CX-Rhodamine labels 25 µg nucleic acid 218,00 PDF
MIR4100 Label IT® Nucleic Acid Labeling Kit, TM-Rhodamine labels 100 µg nucleic acid 544,00 PDF
MIR4125 Label IT® Nucleic Acid Labeling Kit, TM-Rhodamine labels 25 µg nucleic acid 218,00 PDF
MIR3600 Label IT® Nucleic Acid Labeling Kit, Cy®3 labels 100 µg nucleic acid 642,00 PDF
MIR3625 Label IT® Nucleic Acid Labeling Kit, Cy®3 labels 25 µg nucleic acid 258,00 PDF
MIR3700 Label IT® Nucleic Acid Labeling Kit, Cy®5 labels 100 µg nucleic acid 642,00 PDF
MIR3725 Label IT® Nucleic Acid Labeling Kit, Cy®5 labels 25 µg nucleic acid 258,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries..

Download

  • Fluorescent Probes for Protein and Nucleic Acid Labeling Handbook (PDF)