Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Label IT® Plasmid Delivery Controls

Fluorescently labeled plasmid ideal for in vitro and in vivo tracking

LabelIT Cy3 Plasmid Delivery Control

The Label IT® Cy®3 Plasmid Delivery Control Allows Quick Assessment of Delivery Efficiency.
HeLa cells were transfected in serum-containing media with the Label IT® Cy®3 Plasmid Delivery Control (red) using the TransIT®-LT1 Transfection Reagent. Twenty-four hours post-transfection, the cells were fixed, then counterstained to locate the nuclei (blue) and to stain the actin (green).

Visualization of LabelIT Fluorescein Plasmid Delivery Control

Visualization of Label IT® Fluorescein Plasmid Delivery Control in Liver Sections Following Tail Vein Injection.
TransIT®-QR Hydrodynamic Delivery Solution was used to deliver 25 µg of Label IT® Fluorescein Plasmid Control (green) to a mouse using hydrodynamic delivery via the tail vein. One hour post-injection the liver was harvested. Sections were fixed to stain the nuclei (blue) and the actin (red).

Features

  • Sensitive – Easily visualize transfected cells and assess delivery efficiency using fluorescent microscopy
  • Compatible – Suitable for co-delivery experiments with functional plasmids
  • Ease of Use – Supplied as a ready to use 0.5 mg/ml concentration for both in vitro and in vivo tracking studies

Description

  • The Label IT® Plasmid Delivery Control consists of Cy™3- or fluorescein-labeled plasmid DNA (2.7 kb, double stranded, circular plasmid). Each kit is designed as a tool to facilitate visualization and optimization of plasmid DNA delivery, for both in vitro and in vivo applications.
    TransIT® Transfection Reagents (see Related Products Section) are specifically formulated for plasmid DNA delivery to cells. They enable highly efficient plasmid DNA transfection with significantly reduced cytotoxicity compared to cationic liposome-based transfection reagents. Transfections are most effective when carried out in complete growth media, with no media change or serum addition required. TransIT® Transfection Reagents are recommended for all studies involving plasmid DNA transfection including those using the Label IT® Plasmid Delivery Controls.
    In mice, efficient in vivo delivery of plasmid DNA to parenchymal cells in the liver (and reduced expression in the spleen, lung, heart, and kidney) can be obtained using a hydrodynamic injection protocol. The TransIT® In Vivo Delivery System (see Related Products Section) involves a single tail vein injection of complexed DNA and is the recommended procedure for delivery of plasmid DNA, such as the Label IT® Plasmid Delivery Controls, to the liver.

Fluorophore Spectral Characteristics

Fluorophore Excitation Wevelength or λmax(nm) Emission Wavelength (nm)
Cy™3 550 570
Fluorescein 494 518

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR7904 Label IT® Plasmid Delivery Control, Cy®3 10 µg 148,00 PDF
MIR7905 Label IT® Plasmid Delivery Control, Cy®3 100 µg 719,00 PDF
MIR7906 Label IT® Plasmid Delivery Control, Fluorescein 10 µg 114,00 PDF
MIR7907 Label IT® Plasmid Delivery Control, Fluorescein 100 µg 540,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Related Products

Order# Description Amount Price Data Sheet
MIR2300 TransIT®-LT1 Transfection Reagent 1 ml 317,00 PDF
MIR5210 TransIT®-QR Starter Kit 10 injections 120,00 PDF
MIR5310 TransIT®-EE Starter Kit 10 injections 134,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.