The Label IT® Cy®3 Plasmid Delivery Control Allows Quick Assessment of Delivery Efficiency.
HeLa cells were transfected in serum-containing media with the Label IT® Cy®3 Plasmid Delivery Control (red) using the TransIT®-LT1
Transfection Reagent. Twenty-four hours post-transfection, the cells were fixed, then counterstained to locate the nuclei (blue) and to stain the actin (green).
Visualization of Label IT® Fluorescein Plasmid Delivery Control in Liver Sections Following Tail Vein Injection.
TransIT®-QR Hydrodynamic Delivery Solution was used to deliver 25 µg of Label IT® Fluorescein Plasmid Control (green) to a mouse using
hydrodynamic delivery via the tail vein. One hour post-injection the liver was harvested. Sections were fixed to stain the nuclei (blue) and the actin (red).
Features
- Sensitive – Easily visualize transfected cells and assess delivery efficiency using fluorescent microscopy
- Compatible – Suitable for co-delivery experiments with functional plasmids
- Ease of Use – Supplied as a ready to use 0.5 mg/ml concentration for both in vitro and in vivo tracking studies
Description
- The Label IT® Plasmid Delivery Control consists of Cy™3- or fluorescein-labeled plasmid DNA (2.7 kb, double stranded, circular plasmid). Each kit is designed as a tool to
facilitate visualization and optimization of plasmid DNA delivery, for both in vitro and in vivo applications.
TransIT® Transfection Reagents (see Related Products Section) are specifically formulated for plasmid DNA delivery to cells. They enable highly efficient plasmid DNA transfection
with significantly reduced cytotoxicity compared to cationic liposome-based transfection reagents. Transfections are most effective when carried out in complete growth media, with no media change or serum
addition required. TransIT® Transfection Reagents are recommended for all studies involving plasmid DNA transfection including those using the Label IT®
Plasmid Delivery Controls.
In mice, efficient in vivo delivery of plasmid DNA to parenchymal cells in the liver (and reduced expression in the spleen, lung, heart, and kidney) can be obtained using a hydrodynamic injection protocol.
The TransIT® In Vivo Delivery System (see Related Products Section) involves a single tail vein injection of complexed DNA and is the recommended procedure for delivery of
plasmid DNA, such as the Label IT® Plasmid Delivery Controls, to the liver.
Fluorophore Spectral Characteristics
| Fluorophore |
Excitation Wevelength or λmax(nm) |
Emission Wavelength (nm) |
| Cy™3 |
550 |
570 |
| Fluorescein |
494 |
518 |
