Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Label IT® Tracker™ Intracellular Nucleic Acid Localization Kits

Efficient, direct labeling of plasmid DNA for in vitro and in vivo tracking experiments

LabelIT Tracker Kits

The Label IT® Tracker Kits
The Label IT® Tracker labeling reagents are composed of three regions: the label (a fluorophore or biotin) (green), the linker (yellow) which facilitates electrostatic interactions with plasmid DNA and the reactive alkylating group (blue) that covalently attaches the Label IT® reagents to any reactive heteroatom within the plasmid. Attachment of the Label IT® Reagent to plasmids does not alter their structure or affect downstream gene expression, and as such, the same labeled plasmid can be both visualized after transfection by fluorescence microscopy and employed to express the gene of interest on the plasmid. (Mouseover to enlarge)

Tracking of Plasmid Localization and Expression

Tracking of Plasmid Localization and Expression.
COS-7 cells transfected with Label IT® Tracker Cy®5 (blue) labeled pEYFP-nuc and TransIT-LT1 Reagent in serum containing media. Images were acquired at 3, 8, and 20 hours post-transfection. The blue signal indicates the cellular localization of the labeled plasmid, the green signal indicates cellular autofluorescence, and the yellow signal is the expression of the nuclear yellow fluorescent protein (YFP) reporter.

Features

  • Versatile Labeling – Efficiently label and visualize the DNA of your choice.
  • One-step Chemical Method – Easily and consistently control the labeling reactions.
  • High Efficiency Labeling – Optimal visualization of DNA in cells.
  • Superior Tracking – Monitor both subcellular localization and reporter transgene expression following transfection of labeled (plasmid) DNA.

Description

  • The Label IT® Tracker™ Kits are based on Mirus Bio's proprietary nucleic acid labeling technology. The Label IT® Reagents were initially developed by Mirus Bio scientists to monitor the subcellular localization of plasmid DNA in cells following gene delivery. These reagents covalently attach marker molecules to intact nucleic acids in a simple one-step chemical reaction. The simplicity of the highly efficient and non-destructive labeling reaction, and the availability of a variety of labels, including the very sensitive and highly popular Cy™ dyes, makes this the ideal kit to generate labeled DNA for in vitro tracking experiments.
  • Traditional non-radioactive labeling methods (random priming, nick translation) are enzyme mediated and thus inherently difficult to control. In addition, these types of reactions generate labeled products that are not representative of the starting DNA, but rather consist of a series of labeled species over a variable size range. Using the non-enzymatic Label IT® Tracker™ Kits, any plasmid (linear or circular) can be custom labeled before introduction into mammalian cells. This allows for the ideal situation in which expression-competent fluorescently labeled DNA can be visually monitored during the transfection process.

Fluorophore Spectral Characteristics

Fluorophore Excitation Wevelength or λmax(nm) Emission Wavelength (nm)
Cy™3 550 570
Cy™5 649 670
CX-Rhodamine 576 597
TM-Rhodamine 546 576
Fluorescein 494 518

Kit Components:

  • Each Kit contains Label IT® Tracker Reagent, Label IT® Reconstitution Solution, and 10X Labeling Buffer.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
MIR7020 Label IT® Tracker™ Intracellular Nucleic Acid Localization Kit, Cy®3 labels 50-200 µg plasmid DNA 245,00 PDF
MIR7021 Label IT® Tracker™ Intracellular Nucleic Acid Localization Kit, Cy®5 labels 50-200 µg plasmid DNA 245,00 PDF
MIR7022 Label IT® Tracker™ Intracellular Nucleic Acid Localization Kit, CX-Rhodamine labels 50-200 µg plasmid DNA 218,00 PDF
MIR7023 Label IT® Tracker™ Intracellular Nucleic Acid Localization Kit, TM-Rhodamine labels 50-200 µg plasmid DNA 218,00 PDF
MIR7024 Label IT® Tracker™ Intracellular Nucleic Acid Localization Kit, Biotin labels 50-200 µg plasmid DNA 218,00 PDF
MIR7025 Label IT® Tracker™ Intracellular Nucleic Acid Localization Kit, Fluorescein labels 50-200 µg plasmid DNA 218,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Download

  • Technical Report: Intracellular Localization and Expression of Labeled Plasmid DNA Using Label IT® Tracker™ Nucleic Acid Labeling Kits (PDF)
  • Technical Report: Delivery Tools for Neurobiology (PDF)
  • Fluorescent Probes for Protein and Nucleic Acid Labeling Handbook (PDF)