Lipophilic and Membrane Dyes
Normalized fluorescence spectra of DiO, DiI, DiD, and DiR in methanol
- Suitable for neuronal tracing
- Detection of cell-cell fusion and adhesion
- Tracing cell migration during development and after transplantation
- Lipid diffusion in membranes by FRAP (Fluorescence Recovery After Photobleaching)
- Cytotoxicity assays
- Labeling of lipoproteins
- Studying endocytic pathways
- Measuring cAMP in live cells
- Measuring membrane potential
- In collaboration with AnaSpec, USA, MoBiTec is glad to offer a range of well-established and versatile lipophilic membrane fluorescence dyes.
- Lipophilic dyes are used as convenient tools to stain cell membranes of live and fixed cells. Cells tolerate high concentrations of these lipophilic dyes. Furthermore, also lateral diffusion of the dye within the membrane can serve to stain the entire cell, even if the dye is applied locally. Advantage is taken of these properties of lipophilic carbocyanine and aminostyryl dyes in anterograde and retrograde tracing in neuronal cells. Not only cells but also organelles, liposomes, viruses, and lipoproteins can be stained with lipophilic tracers for a wide variety of long-term tracing applications, including cell transplantation, migration, adhesion, and fusion studies.
- DiI has been reported to remain in viable motoneurons up to four weeks in culture and one year in vivo. Staining of neurons in fixed tissue with DiI has been reported to persist for at least two years.
- JC-1 is widely used for measuring membrane potential of mitochondria. The 585/520 fluorescence ratio increases upon cell hyper-polarization.
- Prodan and Laurdan are blue-light excitable environment-sensitive dyes for studying membrane and protein structures.
- For shipping and storage information please click on Order#.
|84704AS||Oxonol VI||100 mg||165,00|
|84713AS||Di-2-ANEPEQ (JPW 1114)||5 mg||157,00|
- Only available in Germany and selected European countries.