Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Fluorescent Probes for Nucleic Acids

Nucleic Acids Stains

APC-alpha-tubulin

Alpha-tubulin in bovine pulmonary artery endothelial cells were detected with mouse anti-alpha-tubulin primary antibody, visualized with Allophycocyanin (APC)-conjugated goat anti-mouse IgG. Nuclei were stained with DAPI (Order# 83210AS).

R-PE-actin

Actins in bovine pulmonary artery endothelial cells were detected with biotin-conjugated phalloidin, visualized with R-phycoerythrin (R-PE)-conjugated streptavidin. Nuclei were stained with Hoechst 33342 (Order# 83218AS).

  • The detection of nucleic acids is an indispensable step for many life science research tasks, such as sequencing, cDNA synthesis and cloning, RNA transcription and transfection, etc. An inaccurate detection of DNA/RNA may cause these techniques to fail. DNA/RNA concentration is often measured by UV absorbance at 260 nm. This method, however, is not as accurate and sensitive as DNA quantitation by using fluorescent dyes. MoBiTec and partners offer a variety of nucleic acid stains for detecting nucleic acids. These stains can be used for DNA quantitation in solutions or in cells. In addition to protein labeling reagents, we also offer reagents for labeling nucleotides and nucleic acids.

Cell-impermeant nucleic acid stains

  • The most popular cell-impermeant nucleic acid stains are 7-AAD, ethidium bromide, and propidium iodide.
  • 7-AAD (7-aminoactinomycin D) is a fluorescent intercalator that binds selectively to GC regions of DNA. 7-AAD/DNA complexes can be excited by argon-ion laser and emit fluorescence with a maxima of 647 nm, making this nucleic acid stain useful for multicolor fluorescence microscopy, confocal laser-scanning microscopy, and flow cytometry.
  • Ethidium bromide and propidium iodide are classic cell-impermeant DNA stains that are structurally similar to phenanthridium intercalators. Both dyes exhibit broad absorption in both UV and visible regions, thus they can be conveniently excited with mercury, xenon-arc lamp or argon-ion laser. This makes them suitable for use in a variety of fluorescence instruments such as fluorescence microscopy, confocal laser-scanning microscopy, and flow cytometry. EthD-1 has higher affinity to DNA, RNA, and oligonucleotides with a large fluorescence enhancement as compared to ethidium bromide.

Cell-permeant nucleic acid stains

  • The most popular cell-permeant nucleic acid stains are DAPI and Hoechst dyes, such as Hoechst 33342 and Hoechst 33258.
  • DAPI is an AT-selective minor groove binder. Binding to dsDNA produces a ~20-fold fluorescence enhancement, emits blue fluorescence upon excitation with mercury-arc lamp or UV lines of the argon-ion laser (Ex/Em = 358/461 nm).
  • DNA-selective Hoechst dyes are classic minor groove-binding dyes. These common nucleus counterstains bind to DNA, and emit blue fluorescence under light excitation (Ex/Em ~ 350/460 nm). They exhibit relatively large Stokes shifts (~110 nm), and are suitable for multicolor labeling experiments. They also exhibit multiple binding modes and distinct fluorescence spectra that are dependent on dye/base pair ratios.
  • Dihydroethidium (also known as hydroethidine), the reduced ethidium derivative, is permeant to live cells. It exhibits blue fluorescence in the cytoplasm. After oxidation by many viable cells, the generated ethidium has red fluorescence upon DNA intercalation (Ex/Em = 518/605 nm).

ORDER INFORMATION

  • For shipping and storage information please click on Order#.

Cell-impermeant nucleic acid stains

Order# Description Amount Price Data Sheet
83201AS 7-AAD 1 mg 106,00 PDF
A1132-1mgAG 7-Amino-Actinomycin D Streptomyces sp. 1 mg 105,00 PDF
A1132-5mgAG 7-Amino-Actinomycin D Streptomyces sp. 5 mg 360,00 PDF
83214AS Ethidium monoazide bromide 5 mg 171,00 PDF
83212AS Propidium iodide 25 mg 75,00 PDF
P2407-1mlAG Propidium Iodide 1 ml 71,00 PDF
83215AS Propidium iodide, 1.0 mg/mL solution in water 10 mL 75,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Cell-permeant nucleic acid stains

Order# Description Amount Price Data Sheet
DPC001AO-PF Liposomal Acridine Orange Dye 1 ml 198,00 PDF
PHC001AO-PF PEGylated Liposomal Acridine Orange Dye 1 ml 198,00 PDF
A1022-5mgAG Actinomycin D 5 mg 74,00 PDF
A1022-100mgAG Actinomycin D 100 mg 512,00 PDF
A1022-10mgAG Actinomycin D 10 mg 129,00 PDF
A1022-25mgAG Actinomycin D 25 mg 292,00 PDF
83210AS DAPI 10 mg 75,00 PDF
83211AS DAPI 25 mg 138,00 PDF
MFPCCFA-211-5 PDF
MFPCCFA-211-10 PDF
MFPCCFA-211-25 PDF
85704AS Dihydroethidium 25 mg 75,00 PDF
85718AS Dihydroethidium 1 mL 176,00 PDF
83219AS Hoechst 33258, 20 mM solution in water 5 mL 75,00 PDF
83218AS Hoechst 33342, 20 mM solution in water 5 mL 75,00 PDF
MFPCCFA-212-5 PDF
MFPCCFA-212-10 PDF
MFPCCFA-212-25 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Download

  • Fluorescent Dyes Brochure (PDF)