Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Glycan Analysis

BlotGlyco™ Glycan Purification and Labeling Kit

Biological Sample
Current situation of Glycomics
Concept of BlotGlyco

Description

  • BlotGlyco™ is a sample preparation kit for glycan analysis of biological samples. Using this kit, it is possible to purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco™ beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it is possible to carry out the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco™ kit is a quite useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.

Features

  • Purification and labeling are completed in about 5 hours using just one spin tube.
  • No special equipment is necessary except a heating block and a small desktop centrifuge. No column chromatography is necessary.
  • Glycans can be purified and labeled from various crude biological samples. Conventional fluorescent labeling reagents (2AB, PA) are applicable. Kit contains a unique labeling reagent for the high sensitive MALDI-TOF MS measurement.
  • By using a 96 well filter plate and a vacuum manifold, as many as 96 samples are processed simultaneously.

Applications

  • Glycosylation analysis of biologics (for research, screening, quality control and production process monitoring)
  • Glycosylation analysis of cells, especially for the differentiation researches of stem cells
  • Combination of glycomics and proteomics (glycans can be analyzed from electrohoresis bands)
  • Glycan biomarker discovery

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
BS-45403Z BlotGlyco 10 1 650,00 PDF
BS-45404Z BlotGlyco 50 1 2925,00 PDF
BS-45405Z BlotGlyco 100 1 5200,01 PDF
BS-45406Z BlotGlyco 100A 1 4888,00 PDF
BS-45407Z BlotGlyco 100B 1 4992,00 PDF
BS-45408Z BlotGlyco 100C 1 4680,00 PDF
BS-45409Z BlotGlyco 100D 1 4888,00 PDF
BS-45410Z BlotGlyco 200C 1 9360,01 PDF
BS-45411Z BlotGlyco 100E 1 4784,00 PDF
BS-45412Z BlotGlyco 200E 1 9360,01 PDF
BS-45413Z BlotGlyco 96 plate B 1 4992,00 PDF
BS-45414Z BlotGlyco 10B 1 585,00 PDF
BS-45415Z BlotGlyco 50B 1 2632,50 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Download

  • BlotGlyco's principle, fundamental data and applications (PDF)
  • BlotGlyco 96well plate kit (PDF)
  • Quick reference of BlotGlyco kit's protocol (PDF)

Literature

  • Furukawa J-i., Shinohara Y., Kuramoto H., Miura Y., Shimaoka H., Kurogochi M., Nakano M., Nishimura S.-I., - A comprehensive approach to structural and functional glycomics based on chemoselectiveglycoblotting and sequential tag conversion Anal. Chem., 80, 1094-1101 (2008)
  • Niikura K., Kamitani R., Kurogochi M., Uematsu R., Shinohara Y., Nakagawa H., Deguchi K., Monde K., Kondo H., Nishimura S.-I., - Versatile Glycoblotting Nanoparticles for High Throughput Protein Glycomics Chem. Eur. J., 11, 3825-3835 (2005)
  • Nishimura S.-I., Niikura K., Kurogochi M., Matsushita T., Fumoto M., Hinou H., Kamitani R., Nakagawa H., Deguchi K., Miura N., Monde K., Kondo H., - High-Throughput Protein Glycomics: Combined Use of ChemoselectiveGlycoblottinandMALDI-TOF/TOF Mass Spectrometry Angew. Chem. Int. Ed., 44, 91-96 (2005)
  • Shinohara Y., Furukawa J.-I., Niikura K., Miura N., Nishimura S.-I., - Direct N-glycan profiling in the presence of tryptic peptides on MALDI-TOF by controlled ion enhancement and suppression upon glycan-selective derivatization Anal. Chem., 76, 6989-6997 (2004)
  • Kita Y., Miura Y., Furukawa J., Nakano M., Shinohara Y., Ohno M., Takimoto A., and Nishimura S.-I., - Quantitative glycomics of human whole serum glycoproteins based on the standardized protocol for liberating N-glycans Mol. Cell. Proteom., 6, 1437-1445 (2007)
  • Miura Y., Shinohara Y., Furukawa J., Nagahori N., and Nishimura S.-I., ‘Rapid and Simple Solid-Phase Esterification of Sialic Acid Residues for Quantitative Glycomics by Mass Spectrometry Chem. Eur. J., 13, 4797- 4804 (2007)
  • Shimaoka H., Kuramoto H., Furukawa J., Miura Y., Kurogochi M., Kita Y., Hinou H., Shinohara Y., and Nishimura S.-I., - One-Pot Solid-Phase Glycoblotting and Probing by Transoximization for High-Throughput Glycomics and Glycoproteomics Chem. Eur. J., 13, 1664-1673 (2007)