Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Autoantibody Assays

Vimentin

Description

  • ELISA-VIDITEST anti-VIMENTIN kits are intended for the detection of IgG and IgM to vimentin in human serum.
  • Vimentin, desmin, glial fibrillary acidic protein and peripherin are four proteins classified as type III intermediate filaments. Among the four proteins vimentin is the most widely distributed, it is a cytoskeletal part in leukocytes, blood vessel, endothelial cells, in some epithelial cells and in cells of mesenchymal origin (fibroblasts). Serum autoantibodies against vimentin were found elevated in patients with neurofibromatosis type I (1), in patients with graft rejections of transplanted organs (2) and in patients with interstitial lung fibrosis (3).

Overview on anti-vimentin assays

Order # Product Method Evaluation No. of tests Sample
ODZ-032 anti-VIMENTIN IgG ELISA qualitative 96 serum
ODZ-033 anti-VIMENTIN IgM ELISA qualitative 96 serum

Complement Factor H

Features

  • High sensitivity/specificity
  • Artificial standard made by covalently linked factor H monoclonal antibody and human IgG
  • Quantitative evaluation of the data

Description

  • ELISA-VIDITEST anti-complement factor H is intended for the quantitative detection of IgG antibodies against human complement factor H in human serum or plasma.
  • Factor H is a complement regulatory glycoprotein that is found in human plasma in concentrations of 300 – 800 mg/l. Autoantibodies that inhibit factor H function cause complement dysregulation and they have been detected in about 10% of patients with atypical hemolytic uremic syndrome (aHUS). Atypical HUS is a clinical syndrome that is characterized by microangiopathic hemolytic anemia, thrombocytopenia and renal failure.
Results of an anti-complement factor H ELISA assay

Results of an anti-complement factor H ELISA assay

Complement factor H assay overview

Order # Product Method Evaluation No. of tests Sample
ODZ-166 anti-complement factor H ELISA qualitative 48 serum

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
ODZ-032 PDF
ODZ-033 anti-VIMENTIN IgM 96 wells 340,00 PDF
ODZ-166 anti-complement factor H 48 wells 240,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Literature

Vimentin

  1. Anti–vimentin antibodies and neuron– specific enolase in children with neurofibromatosis type–1. Kotaska K, Petrak B, Kukacka J, Kraus J, Prusa R. Neuro Endocrinol Lett. 2007 Dec;28(6):761–4.
  2. The autoimmune response to vimentin after renal transplantation in nonhuman primates is immunosuppression dependent. Jonker M, Danskine A, Haanstra K, Wubben J, Kondova I, Kuhn EM, Rose M. Transplantation. 2005 Aug 15;80(3):385–93.
  3. Detection of antivimentin antibody in sera of patients with idiopathic pulmonary fibrosis and non–specific interstitial pneumonia. Yang Y, Fujita J, Bandoh S, Ohtsuki Y, Yamadori I, Yoshinouchi T, Ishida T. Clin Exp Immunol. 2002 Apr;128(1):169–74.

Complement factor H

  1. Anti-factor H autoantibody-associated hemolytic uremic syndrome: review of literature of the autoimmune form of HUS. Dragon-Durey MA, Blanc C, Garnier A, Hofer J, Sethi SK, Zimmerhackl LB. Semin Thromb Hemost. 2010 Sep;36(6):633-40.
  2. Anti-Factor H autoantibodies associated with atypical hemolytic uremic syndrome. Dragon-Durey MA, Loirat C, Cloarec S, Macher MA, Blouin J, Nivet H, Weiss L, Fridman WH, Frémeaux-Bacchi V. J Am Soc Nephrol. 2005 Feb;16(2):555-63.
  3. Factor H autoantibodies in atypical hemolytic uremic syndrome correlate with CFHR1/CFHR3 deficiency. Józsi M, Licht C, Strobel S, Zipfel SL, Richter H, Heinen S, Zipfel PF, Skerka C.Blood. 2008 Feb 1;111(3):1512-4.
  4. Kotaska K, Petrak B, Kukacka J, Kraus J, Prusa R. Anti–vimentin antibodies and neuron– specific enolase in children with neurofibromatosis type–1. Neuro Endocrinol Lett. 2007 Dec;28(6):761–4.
  5. Jonker M, Danskine A, Haanstra K, Wubben J, Kondova I, Kuhn EM, Rose M. The autoimmune response to vimentin after renal transplantation in nonhuman primates is immunosuppression dependent. Transplantation. 2005 Aug 15;80(3):385–93.
  6. Yang Y, Fujita J, Bandoh S, Ohtsuki Y, Yamadori I, Yoshinouchi T, Ishida T. Detection of antivimentin antibody in sera of patients with idiopathic pulmonary fibrosis and non–specific interstitial pneumonia. Clin Exp Immunol. 2002 Apr;128(1):169–74.