Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Phospholipase Research

Autotaxin Activity Assay

Autotaxin Activity Assay

Autotaxin Activity Assay

Features

  • Sample Volume: 400 µl / sample (50 µl per well, 8 wells per sample)
  • Sample Number: 10 samples (in duplicate) / 96 well assay
  • Sample Type: Currently, this kit has been tested for the quantification of autotaxin activity in serum, plasma, ovarian ascites, and cell culture media.

Description

  • The Autotaxin Activity Assay gives a quantitative measurement of autotaxin (ATX) activity in biological samples by measuring changes in fluorescence over time.
  • This assay uses the fluorogenic autotaxin substrate FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophore’s fluorescence. Once autotaxin cleaves FS-3, the fluorophore becomes liberated from the quencher, resulting in increased fluorescence.
  • Because the Autotaxin Activity Assay is not end-point based, it has the advantage of being less susceptible to errors arising from mistiming the addition of components to different sample wells. The final readout is in quantitative units that correct for inconsistencies between different users' equipment.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
K-4100-EC Autotaxin Activity Assay 1 ea 500,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe except for France and Italy.