Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Phosphoinositide 3 (PI3) - Kinase Assays

Phosphoinositide 3 (PI3) - Kinase Assays

Phosphoinositide 3 Kinase Assays

Phosphoinositide 3 (PI3) -Kinase Assays

Features

  • Quick, stable, and sensitive
  • Three convenient formats:
    1. ELISA
    2. Fluorescence Polarization
    3. AlphaScreen

Description

  • The production of PI(3,4,5)P3 by PI3K controls pathways governing cell proliferation, differentiation, apoptosis, migration, neovascularisation and metastasis. PI(3,4,5)P3 regulated signalling is governed both by its formation by PI3-K and by its conversion via the phosphatases, PTEN and SHIP to PI(4,5)P2 or PI(3,4)P2, respectively. The PI3-K pathway is involved in cancer, inflammation, immune response, cardiovascular disease, and diabetes.

Applications

  • In Vitro determination of PI3K activity in cancer tissues and cell lines
  • Compound screening for drug discovery
Assay Format: ELISA Fluorescence Polarization AlphaScreen™
Order#: K-1000s-EC K-1100-EC K-1300-EC
No. of wells/kit: 96 384 384
Application: Detects activity of purified and/or immunoprecipitated PI3-K enzyme Detects PTEN enzyme activity in purified form. Good assay for screening inhibitors. Detects PTEN enzyme activity in purified form. Good assay for screening inhibitors.
Assay detection time: 2.5 hours 15 - 30 min 2 hour
Sensitivity: 0.22 pmol 1.25 pmol (125nM) 0.025 pmol (1nM)
Features and Benefits:
  1. Sensitive, nonradioactive assay
  2. Easy to use, forgiving of human error
  3. Measured by standard plate readers
  1. Quick, sensitive, homogenous assay
  2. Easy to use and stable over time
  3. Can be automated
  1. Simple, sensitive, homogenous assay
  2. Easy to use and stable over time
  3. Can be automated
  4. Requires purchase of AlphScreen™ beads
Assay interference: Substances that absorb strongly in the 450 nm range Cell lysate or substances that significantly alter the viscosity of the assay solution Substances that absorb in the 600 nm range and singlet oxygen quenchers including:
Azide > 0.001%,
Fe2+, Fe3+, Cu2+, Ni2+ > 100 μM,
SO42- > 0.5 mM

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
K-1000s-EC PI3-Kinase Activity ELISA: Pico 1 kit 297,00 PDF
K-1100-EC PI 3-Kinase Activity Fluorescence Polarization Assay 1 ea 410,00 PDF
K-1300-EC PI3-Kinase Activity AlphaScreen® Assay 1 ea 394,00 PDF
K-1800-EC Neutral Sphingomyelinase Activity Assay Kit 1 ea 116,00 PDF
K-1900-EC Sphingosine 1-Phosphate ELISA Kit 1 ea 968,00 PDF
K-3200-EC Acid Sphingomyelinase Assay Kit 1 ea 479,00 PDF
B-0294-1-EC LY294002 (HCl Salt) 5 mg 40,00 PDF
B-0294-2-EC LY294002 (HCl Salt) 25 mg 181,00 PDF
B-0301-1-EC PI 3-Kinase Inhibitor (AS605240) 1 mg 25,00 PDF
B-0301-2-EC PI 3-Kinase Inhibitor (AS605240) 5 mg 56,00 PDF
B-0302-1-EC PI 3-Kinase Inhibitor (AS604850) 5 mg 112,00 PDF
B-0302-2-EC PI 3-Kinase Inhibitor (AS604850) 1 mg 25,00 PDF
B-0303-1-EC PI 3-Kinase Inhibitor (PI-103) 5 mg 109,00 PDF
B-0303-2-EC PI 3-Kinase Inhibitor (PI-103) 1 mg 27,00 PDF
B-0304-1-EC PI 3-Kinase Inhibitor 2 1 mg 46,00 PDF
B-0304-2-EC PI 3-Kinase Inhibitor 2 5 mg 198,00 PDF
B-0305-1-EC IC87114 (PI3-K delta inhibitor) 1 mg 61,00 PDF
B-0305-2-EC IC87114 (PI3-K delta inhibitor) 5 mg 116,00 PDF
B-0305-3-EC IC87114 (PI3-K delta inhibitor) 25 mg 502,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe except for France and Italy.

Download

  • Probes for Investigating Signal Transduction (Brochure) (PDF)