Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

PIP Mass Assays

Non-Radioactive PIP Mass Assays

Non-radioactive PIP mass assays

Non-Radioactive PIP Mass Assays

Features

  • Non-radioactive PIP mass assays
  • Quick, simple protocols
  • Lipid protein overlay fomat
  • ELISA plate format

Description

  • The PIP Mass assays were designed to help researchers quantify PIP mass quickly and easily. They offer substantial time and cost savings when compared to radioactive methods. The production of PI(3,4,5)P3 from PI(4,5)P2 by type-1 PI3-kinases (PI3-K) is important in multiple cell signaling pathways.
  • Typically, experiments to measure PI3-K activity have involved phosphorylation of a phosphoinositide substrate using 32P, then extraction of radioactive products, and separation using thin-layer chromatography. The assay plate method developed by Echelon Biosciences, Inc. allows the user to determine PI3-K activity by measuring the amount of PI(3,4,5)P3 extracted from cells by means of standard ELISA format, eliminating the need for radioactivity, organic solvents, and thin layer chromatography.
  • The assay is a competitive ELISA in which the signal is inversely proportional to the amount of PI(3,4,5)P3 produced. Once PI(3,4,5)P3 has been extracted from cells samples, it is first incubated with a PI(3,4,5)P3 detector protein, then added to the PI(3,4,5)P3-coated microplate for competitive binding. A peroxidase-linked secondary detection reagent and colorimetric substrate is used to detect PI(3,4,5)P3 detector protein binding to the plate. The colorimetric signal is inversely proportional to the amount of PI(3,4,5)P3 extracted from cells.
  • The assay is sensitive to 1 pmol PIP3, and requires approximately ~3 x 106 cells per data point depending on the experimental system used.

Applications

  • PIP quantitation from cell sample
  • Determination of PI3K activity and its related growth signalling pathway
How to determine PIP3 Mass

How to determine PIP3 Mass

PIP3 Mass ELISA

PIP3 Mass ELISA

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
K-3300-EC PI(3)P Mass ELISA 1 kit 667,00 PDF
K-3800-EC PI(3,4P)P2 Mass ELISA 1 ea 667,00 PDF
K-4000E-EC PI(4)P Mass ELISA Kit 1 kit 611,00 PDF
K-4500-EC PI(4,5)2 Mass ELISA 1 ea 697,00 PDF
K-2500s-EC PIP3 Mass ELISA 1 ea 605,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in Europe except for France and Italy.