Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology


Neurofilament Assay



  • Sensitive and precise
  • Quantitative data evaluation
  • One-step ELISA - minimal hands-on time requirements
  • No sample dilution before assay
  • Incubation buffer that minimizes heterophil antibody interference


  • ELISA-VIDITEST pNF-H is intended to measure the concentration of phosphorylated forms of heavy neurofilaments in peripheral blood and cerebrospinal fluid. The sandwich ELISA uses the mouse monoclonal antibody NF01 that binds to the phosphorylated epitopes on heavy neurofilaments and mouse monoclonal antibody NF05 that reacts equally with phosphorylated and non-phosphorylated forms of heavy neurofilaments. Phosphorylated heavy neurofilaments were detected in higher concentrations in diseases that involve central nervous system damage (Shaw et al. 2005). The kit except the human pNF-H, detects porcine, bovine and rat pNF-H but not mouse pNF-H.

  • Neurofilamets are the main cytoskeletal constituents in neuronal cells. They are important for maintaining the structural integrity and caliber of axons and dendrites thereby influencing the conduction velocity of nerve impulses. The neurofilament chains are divided into three groups according to their molecular size, neurofilament light (NF-L), neurofilament medium (NF-M), neurofilament heavy (NF-H). NF-L is the quantitatively most common filament with a molar ratio of 4:2:1 (NF-L: NF-M: NF-H). Phosphorylation of the C-terminal part of heavy and medium neurofilaments shows topological dependence, neurofilaments in axons are heavily phosphorylated, cross-linked and spatially organized, whereas neurofilaments found in neuronal body and in dendrites possess low degree of phosphorylation, the crosslinking level is low and their orientation is random.

Assay Overview

Order # Product Method Evaluation No. of
Sample Limit of detection
ODZ-037 pNF-H ELISA quantitative 96 serum, plasma, cerebrospinal fluid 31 pg/ml


  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
ODZ-037 pNF-H 96 wells 623,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.


  • Shaw G, Yang C, Ellis R, Anderson K, Parker Mickle J, Scheff S, Pike B,Anderson DK, Howland DR. Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 2005 Nov 4;336(4):1268-77.
  • Porchet R, Probst A, Draberova E, Draber P, Riederer IM, Riederer BM.: Differential subcellular localization of phosphorylated neurofilament and tau proteins in degenerating neurons of the human entorhinal cortex. Neuroreport. 2003 May 23;14(7):929-33.