Human Anti-PEth Autologous IgG ELISA
Alcohol-Related Biomarker Detection
Anti-PEth Autologous IgG ELISA
The quantity of Anti-PEth Autologous IgG in serum can be estimated by comparing the values from the wells containing the diluted samples with the values from the standard curve.
Figure 1 (top right): Plot the absorbance values obtained vs. amount of Anti-PEth IgG per standard to generate a standard curve.
Figure 2 (down right): Determine where the diluted sample values lie on the curve to obtain a measure of Anti-PEth IgG in your samples.
- New exclusive assay for alcohol research
- Simple to use
- All reagents included
- Consistent results
- Human Anti-PEth Autologous IgG ELISA is a direct assay in which the signal is proportional to the amount of autologous anti-PEth IgG contained in the sample. The serum (or plasma) sample is added to a PEth-coated plate for binding. A peroxidase-linked secondary detector and colorimetric substrate are used to detect the amount of anti-PEth antibody bound to the plate. The colorimetric signal is read at absorbance 450 nm and is directly proportional to the amount of autologous anti-PEth IgG in the sample.
- Ethanol (EtOH) has multiple effects on humans and other animals. Alcohol addiction and organ damage are active areas of research with a need for research reagents and tools. Phosphatidylethanol (PEth) is a direct metabolite of EtOH and has been described as a long-lived biomarker for alcohol ingestion. It is possible that PEth is involved in the neurological, protective, and harmful effects of alcohol, but the mechanisms are still being explored.
- Autologous anti-PEth antibodies are found in lower abundance in serum from heavier drinkers. An inverse relationship between anti-PEth IgG levels and alcohol ingestion has demonstrated a high degree of correlation with ingested alcohol. Measuring these autologous anti-PEth IgG levels may serve as an effective biomarker in monitoring alcohol use.
- Freeman, W. M.; Vrana, K. E. Future prospects for biomarkers of alcohol consumption and alcohol-induced disorders. Alcohol Clin Exp Res 2010, 34, 946-54.
- Viel, G.; et al. Phosphatidylethanol in blood as a marker of chronic alcohol use: a systematic review and meta-analysis. Int J Mol Sci 2012, 13, 14788-812.
- Wurst, F. M.; et al. Phosphatidylethanol: normalization during detoxification, gender aspects and correlation with other biomarkers and self-reports. Addict Biol 2010, 15, 88-95.
- Esteban-Pretel, G.; et al. Polyphosphoinositide metabolism and Golgi complex morphology in hippocampal neurons in primary culture is altered by chronic ethanol exposure. Alcohol Alcohol 2012, 48, 15-27.
- Foster, D. A.; et al. Reduced mortality and moderate alcohol consumption: the phospholipase D-mTOR connection. Cell Cycle 2010, 9, 1291-4.
- Pannequin, J.; et al. Phosphatidylethanol accumulation promotes intestinal hyperplasia by inducing ZONAB-mediated cell density increase in response to chronic ethanol exposure. Mol Cancer Res 2007, 5, 1147-57.
- Nissinen, A.E.; et al. Low plasma antibodies specific for phosphatidylethanol in alcohol abusers and patients with alcoholic pancreatitis. Addict Biol, 2011. 17(6): p. 1057-67.