Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

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Infectious Serology: Polyomavirus JC and Polyomavirus BK

Detection of Specific IgG Antibodies against Polyomavirus JC

Polyomavirus

Polyomavirus

Serology

Features

  • ELISA kit for the detection of species-specific IgG antibodies against polyomavirus JC (JCV) in human serum and plasma
  • Quantification using 5 standards
  • Incubation times 60’/60’/10’
  • Incubation at laboratory temperature
  • CE-IVD certification

Description

  • ELISA-VIDITEST anti-JCV is an ELISA kit for the detection of specific IgG antibodies against JCV in human serum and plasma. ELISA-VIDITEST anti-JCV is easy to use and has high sensitivity and specificity. The kit contains ready-to-use conjugate and controls and standard VIDIA buffers, which are interchangeable between VIDIA ELISA kits.

  • From 50% to 60% of population is infected by polyomavirus JC (JCV) during childhood. Infection is without any symptoms and later continues to the latent phase, which is characterised by long-term persistence of anamnestic IgG antibodies in serum. Virus can repeatedly reactivate in latently infected people or the reinfection by other serotypes can occur. Reactivation/reinfection can be accompanied by temporary viremia or asymptomatic excretion in urine, in rare cases of immunocompromised patients it can cause infection of central nervous system - progressive multifocal leukoencephalopathy (PML). The presence of anti-JCV antibodies is one of the risk factors of PML outbreak in patients treated with natalizumab. Significant increase or high anti-JCV antibody level can indicate reinfection or reactivation in these patients.

Application

  • Diagnostics of diseases associated with polyomavirus JC
  • Monitoring of progressive multifocal leukoencephalopathy risk in patients with immunomodulatory therapy (natalizumab)

Advantages

  • Recombinant antigens do not cross-react with other polyomaviruses
  • Quantitative data evaluation – monitoring of antibody concentration
  • High sensitivity (95%) and specificity (96%)
  • Ready-to-use HRP conjugate and controls
  • Interchangeable VIDIA buffers

Polyomavirus BK

ELISA-VIDITEST anti-BKV

Features

  • Microtiter plate contains a mixture of highly specific antigens – no cross-reactivities with other polyomaviruses
  • Semiquantitative data evaluation
  • High sensitivity and specificity
  • Ready-to-use HRP conjugate and controls

Description

  • ELISA-VIDITEST anti-BKV IgG kit is intended for the detection of specific IgG antibodies to polyomavirus BK (BKV) in human serum and plasma. Recombinant antigens used in the kit do not cross-react with other polyomaviruses (polyomavirus JC, Merkel cell polyomavirus). The kit is used for serological diagnostics of diseases caused or associated with BKV (e.g., BK-viral nephropathy, haemorrhagic cystitis, urethral stenosis, infections of upper and lower respiratory tract mainly in immunodeficient patients) and for risk assessment of infection transmission and subsequent complications in graft acceptors.

  • Anti-BKV antibodies are present in 50% to 80% of adult population. Primary infection occurs mostly during childhood and in most of the cases it is asymptomatic or brings on an acute respiratory disease and then continues to the latent phase, which is characterised by long-term presence of anamnestic IgG antibodies in serum. In latently infected persons the virus can repeatedly reactivate or they can be re-infected by other BKV serotypes. Reactivation/reinfection can be accompanied by temporary viremia or viruria and in immunodeficient persons it can cause various diseases of urinary tract (haemorrhagic cystitis, urethral stenosis), kidney (BK-viral nephropathy), central nervous system (encephalitis, polyradiculoneuritis), lung (intersticial pneumonitis) or vasculitis. Absence of anti-BKV antibodies may indicate patient’s susceptibility to primary infection, which is connected with increased complication risk. Primary infection can be diagnosed using anti-BKV IgG seroconversion. Significant increase of antibody level in paired serum/plasma samples can be a sign of reinfection or virus reactivation.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
ODZ-262 anti-JCV IgG 96 wells 203,58 PDF
ODZ-405 anti-BKV JgG 96 wells 278,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Download

  • Vidia Polyomavirus JC Flyer (PDF)
  • Vidia Product Catalog (PDF)