Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Imaging Chambers

Imaging Chambers and Imaging Chambers CG

Features

  • Extraordinary planarity of the imaging plane, even during temperature shifts
  • High temperature stability, suitable for FISH
  • High chemical resistance, fixation with acetone enabled
  • Bottom glasses can be obtained with minimal glue residues
  • All Imaging Chambers are delivered with lid and are placed on a Slide Tray Plate

Applications

  • Laser scanning confocal microscopy (LSM)
  • Total internal reflection fluorescence (TIRF)
  • Differential interference contrast (DIC)
  • Fluorescence correlation spectroscopy (FCS)
  • Fluorescence resonance energy transfer (FRET)
  • Fluorescence recovery after photobleaching (FRAP)
  • Low intensity fluorescence
  • Fluorescence in-situ hybridization (FISH)
  • Immunohistology

Available with microscope slides or cover glass slides (Imaging Chamber CG) as bottom structure

  • Made from high performance polymers and sealed against the glass bottoms by biological inert silicone adhesive. At the end of the experiments the glasses can be detached from the polymer chambers with minimal glue residue.
  • High performance polymer of chamber bodies and lids withstands temperatures up to 90 °C and is resistant against acetone. Full products are suitable for in-situ hybridization technologies as well as for a wide range of fixation and staining protocols.
  • Designed for high resolution live cell microscopy techniques. Imaging Chambers with microscope slides can be used with long distance objectives. After disassembly the slides can be covered with cover glasses and are then also suitable for high resolution immersion microscopy.
  • Cell adhesion, spreading and distribution on top of the glass surface is improved due to our proprietary plasma surface modifications.
  • Available in 1 well, 2 well, 4 well and 8 well format.
Imaging Chambers with microscope slides

Imaging Chambers with microscope slides

Imaging Chambers in Slide Tray Plate

Imaging Chambers in Slide Tray Plate

Technical data of Cover Glass (CG) and Microscope Slides

Cover glass slide properties Microsope slide properties
Thickness 170 µm +/- 10%   1 mm  
Glass type Borosilicate glass, hydrolytic class I Soda lime, smoothed corners, frosted ends
Refractive index 1.52   1.52  
Abbe's number 50 50
Length 58 mm 58 mm
Width 26 mm 26 mm
Surface quality TC (Tissue Culture Quality) TC (Tissue Culture Quality)

Technical data of Imaging Chambers and Imaging Chambers CG

Order# 8001,
8011
8002,
8012
8004,
8014
8008,
8018
Number of wells / chamber 1 2 4 8
Chamber height 10 mm 10 mm 10 mm 10 mm  
Imaging area / well 10.84 cm2 5.04 cm2 2.14 cm2 0.88 cm2 
Total volume / well 11 ml 5.5 ml 2.2 ml 0.9 ml  
Suggested working volume / well 2000 µl 1000 µl 500 µl 300 µl
Total chamber length 58 mm 58 mm 58 mm 58 mm
Total chamber width 26 mm 26 mm 26 mm 26 mm

TC = Tissue Culture, aminanted surface, suitable for most adherent cell lines (e.g. HEK U293, HepG2, MCF-7). Sensitive cell lines (e.g. PC-12) and some primary cell types benefit from additional surface coatings with ECM proteins or equivalent coatings.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
8011-16 Imaging Chamber 1 16 50,00 PDF
8011-80 Imaging Chamber 1 80 229,00 PDF
8012-16 Imaging Chamber 2 16 51,00 PDF
8012-80 Imaging Chamber 2 80 236,00 PDF
8014-16 Imaging Chamber 4 16 54,00 PDF
8014-80 Imaging Chamber 4 80 243,00 PDF
8018-16 Imaging Chamber 8 16 57,00 PDF
8018-80 Imaging Chamber 8 80 257,00 PDF
8001-16 Imaging Chamber 1 CG 16 64,00 PDF
8001-80 Imaging Chamber 1 CG 80 286,00 PDF
8002-16 Imaging Chamber 2 CG 16 67,00 PDF
8002-80 Imaging Chamber 2 CG 80 300,00 PDF
8004-16 Imaging Chamber 4 CG 16 69,00 PDF
8004-80 Imaging Chamber 4 CG 80 307,00 PDF
8008-16 Imaging Chamber 8 CG 16 70,00 PDF
8008-80 Imaging Chamber 8 CG 80 321,00 PDF
  • All prices are in EURO excl. VAT and shipping. For further pricing and order information please ask your local distributor.

Download

  • Scientific Labware for cell-based imaging, assays & arrays (PDF)