Innovative Tools for Molecular and Cell Biology

Innovative Tools for Molecular and Cell Biology

Products

Non-UV Transilluminator: The Dark Reader®

Non-UV Transilluminator: The Dark Reader®

Dark Reader

Dark Reader®

Features

  • Very safe to use with essentially zero UV output and no risk of sunburn or eye damage. The extent of DNA sample damage is drastically reduced and cloning is increased over 100-fold.
  • No damage to precious DNA samples
  • Very Sensitive: Dark Reader® apparatus is at least as sensitive as UV for the detection of many popular dyes and can be used to see, directly by eye, less than 100 pg of stained DNA.
  • 100% compatible with photographic and video documentation systems
  • Excitation light passes through most plastics and glass, allowing detection in 96well plates and viewing of DNA fragments during gel electrophoresis and even in transgenic plants and animals.

Description

  • The Dark Reader® is a Non-UV Transilluminator that uses a novel patent-pending technology to visualize fluorescently stained DNA, RNA, proteins, and other biological samples. Dark Reader® devices use a visible light source that excites fluorophores between about 420 and 500 nm. This range includes many popular dyes such as SYBR® Green, SYBR® Gold, SYPRO® Orange, fluorescein and its derivatives like Alexa® Fluor or red-shifted GFP variants.

The Dark Reader™ uses 2 Filters to reveal Fluorescence

A comparison of the output of the Dark Reader and a 312 nm UV transilluminator

A comparison of the output of the Dark Reader® and a 312 nm UV transilluminator.

Schematical view of the optical arrangement

Schematical view of the optical arrangement.

  • If visible light is used for excitation of a fluorophore, any fluorescence from the sample is not directly detectable by the naked eye due to the large amount of incident light from the light source itself that reaches the observer. The Dark Reader® achieves the removal of incident light in 2 steps. The first filter is between the light source and the DNA. This removes any green and red components from the lamp and allows through to the DNA only blue excitation light. A second filter is placed between the DNA and observer that removes the blue incident light but allows passage of the red and green fluorescent components.

Dark Reader™ Transilluminators

The Dark Reader® Transilluminators use pure visible blue light for excitation so no ultraviolet light is emitted. With the Transilluminators you will be able to view a wide range of fluorescent samples.

We currently have 4 transilluminators of different sizes available. Along with the Dark Reader® Transilluminators you get a compatible amber screen that absorbs the blue excitation light after illuminating the dye so you can clearly see the fluorescence. Also, the amber screen can be used as camera filter. Other included accessories are viewing glasses that absorb the blue light like the amber screen. They are convenient for cutting bands out of the gel.

Dark Reader (order# DR-22A)

Dark Reader®
(order# DR-22A)

Dark Reader (order#DR-46B)

Dark Reader®
(order#DR-46B)

Dark Reader (order# DR-89X)

Dark Reader®
(order# DR-89X)

Dark Reader (order# DR-195M)

Dark Reader®
(order# DR-195M)

Dark Reader® Transilluminators:

DR-22A Transilluminator

  • Viewing surface dimensions: 13 x 12 cm
  • Overall dimensions: 16 x 14.5 x 7 cm
  • Weight: 0.45 kg approx
  • Optical: Multiple blue LEDs
  • Included accessories: Amber screen and DR viewing glasses.

DR-46B Transilluminator

  • Viewing surface dimensions: 19 x 15 cm
  • Overall dimensions: 22.5 x 18.5 x 8 cm
  • Weight: 0.6 kg approx
  • Optical: Multiple blue LEDs
  • Included accessories: Amber screen and DR viewing glasses.

DR-89X Transilluminator

  • Viewing surface dimensions: 25 x 22 cm
  • Overall dimensions: 29.5 x 26 x 12 cm
  • Weight: 2.5 kg approx
  • Optical: Multiple blue LEDs
  • Included accessories: Amber screen and DR viewing glasses.

DR-195M Transilluminator

  • Viewing surface dimensions: 46 x 30.5 cm
  • Overall dimensions: 50.8 x 34.8 x 12.5 cm
  • Weight: 6.4 kg approx
  • Optical: 2 x 32W compact fluorescent lamps
  • Included accessories: Amber screen and DR viewing glasses.

Lamps

Dark Reader® hand-held Lamps enable the viewing of fluorophors in a variety of settings far beyond simple electrophoresis gels. Researchers are using our Lamps to monitor viral infection in whole plants, bacterial metabolism of toxic waste, fish distribution in river systems, GFP expression in mice and many, many other applications.
Both the Hand Lamp and the Spot Lamp come provided with a free pair of viewing glasses which are convenient for cutting bands out of gels. The glasses are used to block the blue light from the Dark Reader® so that the fluorescence from the sample can be clearly seen.

We offer two versions of lamps:

DR Hand Lamp (order# DRHL-34T)

DR Hand Lamp
(order# DRHL-34T)

DR Hand Lamp (DRHL-34T):

All Dark Reader® Lamps use pure visible blue light as the excitation source and there is NO damaging and dangerous UV radiation to worry about. The blue light is especially suited for following fluorophors such as GFP and RFP as well as DNA stains such as GelGreen and GelStar.

The Hand Lamp (DRHL-34T) smoothly illuminates an area about 20 cm diameter (from a distance of 30 cm) and is ideal for use with bigger gels and larger samples in general.

DRHL-34T Hand Lamp Specifications:
  • Overall dimensions: 17 x 16 cm (l x d)
  • Illumination area: ~ 20 cm diameter
  • Weight: 450 g approx
  • Electrical: 12 V wall transformer
  • Optical: 6 x blue high power LEDs
DR Spot Lamp (order# DRSL-9S)

DR Spot Lamp
(order# DRSL-9S)

DR Spot Lamp (DRSL-9S):

The new Dark Reader® Spot Lamp (DRSL-9S) generates a narrower beam about twice as intense as the Hand Lamp and covers an area about 5 cm in diameter - useful for minigels and smaller samples.

The remarkable efficiency of the Spot Lamp means that it can be powered by a small battery pack, allowing the Lamp to be easily used in remote locations.

DRSL-9S Spot Lamp Specifications:
  • Overall dimensions: 7.5 x 7.5 x 6.5 cm (l x w x h)
  • Illumination area: ~ 5 cm diameter
  • Weight: 110 g approx
  • Electrical: 12 V wall transformer
  • Optical: 3 x blue high power LEDs

Dark Reader® Gel Head

DarkReader GelHead (order# DRDG-345)

Dark Reader® GelHead
(order# DRDG-345)

The Dark Reader® GelHead (DRDG-345) is a unique electrophoresis unit with an integrated Transilluminator. With the Dark Reader® GelHead you can monitor the progress of DNA fraction in real time until your DNA bands of interest are separated. Depending on the complexity of the sample, this can occur in 30 minutes or less.

DRDG-345 GelHead Specifications

  • Overall dimensions: 27 x 20.5 x 13 cm (length x width x height)
  • Gel bed dimensions: 12 x 8 x 0.5 cm
  • Weight: 1.7 kg approx
  • Electrical (lamp): 12V DC. Wall adapter included. (A separate power supply to provide the voltage across the gel is required)
  • Optical: Single 9W lamp
  • Combs: 17 well (~15 µL), 10 well (~30 µL) and 6 well (~60 µL). Two combs per gel for up to 34 samples.

Accessories

DR Glasses 'compact' (order# DRAG-16)

DR Glasses "compact"
(order# DRAG-16)

Viewing glasses

All Dark Reader® models are provided with a free pair of viewing glasses, as well as an amber screen. Both the glasses and the screen function to block the blue excitation light generated by the Dark Reader, so that the sample fluorescence can be clearly seen. The glasses are particularly useful for cutting bands out of gels.

Model DRAG-16 are compact and can be worn over most styles of prescription glasses.

DR Glasses 'classic' (order# DRAG-15)

DR Glasses "classic"
(order# DRAG-15)

The `classic` DRAG-15 style viewing glasses are still available when ordered as a separate item.

Camera Filters

Camera Filters

Camera Filters

The filters offered by Clare Chemical have exactly the same optical properties as the amber screen. They have been introduced for those researchers who prefer to operate their Dark Reader® transilluminators without the amber screen. Substituting the screen with a camera filter can be helpful, for example, in humid climates where condensation tends to form more rapidly under the screen. Also, if you wish to photograph materials illuminated by the Hand Lamp or Spot Lamp, you will need a separate filter. In the case of the GelHead, the amber screen is an integral part of the electrophoresis apparatus and must be in place for the unit to function.

Attaching a filter to a digital camera usually involves additional accessories. For example, with most cameras, a 'lens adapter tube' is required. The tube screws to the body of the camera, over the lens. A filter can then be screwed onto the other end of the tube. In some cases, the thread size of the tube is 'non-standard' and so it is also necessary to purchase a 'step-up ring'. The ring is threaded at both ends: one end attaches to the tube and the other end will accept a standard-size filter. Tubes and rings are purchased from the camera supplier.

Note: a separate camera filter is not needed for the photography or imaging of fluorescent samples using any of our transilluminators or GelHead! All these units are equipped with an amber screen that effectively functions as a camera filter.

ORDER INFORMATION

  • For shipping and storage information please click on Order#.
Order# Description Amount Price Data Sheet
DR-22A 12 x 13 cm DR Transilluminator 1 575,00 PDF
DR-46B 15 x 19 cm DR Transilluminator 1 828,00 PDF
DR-89X 22 x 25 cm DR transilluminator 1 1168,00 PDF
DR-195M 30 x 46 cm DR Transilluminator 1 2302,00 PDF
DRDG-345 DR GelHead 1 1356,00 PDF
DRHL-34T DR Hand Lamp 1 645,00 PDF
DRSL-9S DR Spot Lamp 1 516,00 PDF
DRAG-16 DR glasses 'compact' 1 100,00 PDF
DRAG-15 DR glasses 'classic' 1 100,00 PDF
DRAF-370 DR 37mm screw-on camera filter (50\% discount for Dark Reader customer) 1 184,00 PDF
DRAF-405 DR 40.5mm screw-on camera filter (50\% discount for Dark Reader customer) 1 188,00 PDF
DRAF-460 DR 46mm screw-on camera filter (50\% discount for Dark Reader customer) 1 192,00 PDF
DRAF-490 DR 49mm screw-on camera filter (50\% discount for Dark Reader customer) 1 197,00 PDF
DRAF-520 DR 52mm screw-on camera filter (50\% discount for Dark Reader customer) 1 201,00 PDF
DRAF-550 DR 55mm screw-on camera filter (50\% discount for Dark Reader customer) 1 205,00 PDF
DRAF-580 DR 58mm screw-on camera filter (50\% discount for Dark Reader customer) 1 210,00 PDF
DRAF-620 DR 62mm screw-on camera filter (50\% discount for Dark Reader customer) 1 214,00 PDF
DRAF-670 DR 67mm screw-on camera filter (50\% discount for Dark Reader customer) 1 218,00 PDF
DRAF-07 DR filter 75x75mm (50\% discount for Dark Reader customer) 1 184,00 PDF
DRAC-301 Comb set for GelHead345 1 48,00 PDF
DRAL-06 Lamp for 45,88,& 345 1 33,00 PDF
DRAL-13 Lamp for DR195 1 62,00 PDF
DRAA-46 Amber screen for DR46 1 178,00 PDF
DRAB-46 Blue screen for DR46 1 221,00 PDF
DRAA-88 Amber screen for DR88 1 228,00 PDF
DRAB-88 Blue screen for DR88 1 284,00 PDF
DRAA-195 Amber screen for DR195 1 321,00 PDF
DRAB-195 Blue screen for DR195 1 370,00 PDF
DRAA-22 Amber screen for DR22 1 119,00 PDF
DRAB-22 Blue screen for DR22 1 150,00 PDF
  • All prices are in EURO excl. VAT and shipping. Only available in selected countries.

Download

  • Dark Reader® - Handbook (PDF)

Literature

  • Reference Genetic Engineering News (PDF)
  • Reference Modern Drug Discovery (PDF)