|Description:||Antibacterial Drug Target ׀ DNA decatenation is an essential process during DNA replication in the cells. The DNA decatenation reaction was carried out by topoisomerase IV (the parC-parE complex) in bacteria. The DNA decatenation reaction converts the concatenated DNA into decatenated DNA. The Gel-Based E. coli Topoisomerase IV DNA Decatenation Assay is based on the principle that the decatenated DNA and concatenated DNA are separated by agarose gel electrophoresis. This product includes all the reagents for 100 samples in gel-based assays of E. coli topoisomerase IV DNA decatenation activity. It includes 300 µl of 10 x Buffer, 205 µl of 60 mg/ml concatenated DNA (kDNA), 205 µl of 10 mM ATP, 550 µl of 5 x Gel loading buffer and 22 µl of 10 µM E.coli topoisomerase IV (the parC-parE complex).|
|Unit Size:||100 assays|
|Shipping:||Blue Ice (-20°C)|
|More information:||Go to webpage|
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