|Description:||General Drug Target ׀ The MicroMolar Universal Kinase Assay Kit is based on measurement of ADP generated from the kinase reaction. It is used to measure the activities of purified kinases producing ADP at concentrations ranging from 0.1 mM to 10 mM. The assay is fluorescence-based and can be carried out using regular black or white 96-well or 384-well plates or micro-cuvettes. It is important to avoid using unnecessarily high ATP concentrations in the kinase assays for the following reasons. One is to allow the assay to detect ATP-competitive inhibitors. The other is to avoid a high ADP background from ADP contamination in the ATP samples. It is recommended to use ATP concentrations at 20 µM (micromolar). This product includes 4000 ul of 10 x assay buffer, 350 ul of 100 x ATP, 350 ul of 100 x MUK Reagent A, 350 ul of 100 x MUK Reagent B, 200 ul of 1 mM ADP and 3500 ul of 10 x fluorescence dye for 1000 assays in a 384-well assay format.|
|Unit Size:||1000 assays|
|Shipping:||Blue Ice (-20°C)|
|More information:||Go to webpage|
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